Lipid content disturbance in the arbuscular mycorrhizal, Glomus irregulare grown in monoxenic conditions under PAHs pollution

Debiane, Djouher; Calonne, Maryline; Fontaine, Joël; Laruelle, Frédéric; Grandmougin-Ferjani, Anne; Sahraoui, Anissa Lounès-Hadj

doi:10.1016/j.funbio.2011.06.003

Cited by 29 publications

(20 citation statements)

References 95 publications

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“…PC quantity decreased in a similar manner to that reported by Debiane et al (2011), which was likely due to PC degradation and not to a decrease in its synthesis, as previously hypothesized. Anthracene exposure caused an oxidative stress revealed by an increase in lipid peroxidation biomarker production, malondialdehyde, in R. irregularis extraradical structures (Debiane et al 2011).…”

Section: Discussionsupporting

confidence: 85%

“…As proposed by Fontaine et al (2001b), in germinating spores, sterols required for membrane synthesis may be provided by the hydrolysis of sterol esters, which represent 40% of the total sterols in the R. irregularis extraradical mycelium. In the extraradical AMF, these sterol storage lipids may be hydrolyzed to offset the decrease in 4-demethylsterol content in the presence of anthracene in R. irregularis, as reported by Debiane et al (2011).…”

Section: Control Anthracenementioning

confidence: 76%

“…Several studies have reported that this PAH affects the main steps of the AMF life cycle. AMF spore germination, intraradical development, extraradical hyphae elongation, and sporulation were drastically reduced by anthracene (Verdin et al 2006;Debiane et al 2008Debiane et al , 2011.…”

Section: Introductionmentioning

confidence: 96%

“…More recently, Debiane et al (2011) showed that anthracene fungitoxicity results from induction of oxidative stress. It was also demonstrated that production of malondialdehyde (a lipid peroxidation biomarker), as well as lipid content disturbance, occurred in R. irregularis when grown in the presence of anthracene.…”

Section: Introductionmentioning

confidence: 99%

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Impact of anthracene on the arbuscular mycorrhizal fungus lipid metabolism

Calonne

Fontaine

DebianeD.

et al. 2014

Botany

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Anthracene, a low-molecular-weight polycyclic aromatic hydrocarbon (PAH) originating mainly from anthropogenic activities, represents one of the major persistent organic pollutants frequently detected in polluted soils. A few studies have reported the negative effect of PAH on the main steps of the arbuscular mycorrhizal fungi (AMF) life cycle resulting from lipid peroxidation; however, little is known regarding the impact of anthracene on extraradical AMF lipid metabolism. Radiolabelling experiments showed significant decreases of [1-14 C]acetate incorporation into the sterol precursors (4,4-dimethylsterols and 4␣-methylsterols) and in the total phospholipids (PL) of Rhizophagus irregularis (Blaszk., Wubet, Renker & Buscot) extraradical mycelium when grown in the presence of anthracene. These findings suggested a slowing down of the sterol and total PL biosynthesis pathways in AMF treated with anthracene. The negative impact of the organic pollutant on AMF membrane lipid biosynthesis may explain the growth inhibition of the fungus after PAH exposure. This study increases the understanding of the biochemical mechanisms involved in PAH ecotoxicity on AMF.Résumé : L'anthracène, hydrocarbure aromatique polycyclique (HAP) de faible poids moléculaire d'origine principalement anthropique, fait partie des polluants organiques persistants majeurs fréquemment rencontrés dans les sols pollués. Quelques travaux ont démontré l'effet négatif de cet HAP sur le développement du champignon mycorhizien arbusculaire (CMA), Rhizophagus irregularis (Blaszk.,Wubet, Renker & Buscot), attribué, en partie, à la peroxydation lipidique. En revanche, peu de données concernant l'impact de l'anthracène sur le métabolisme lipidique du mycélium extra-racinaire des CMA sont actuellement disponibles. Des expériences de radiomarquage ont montré des diminutions significatives de l'incorporation de l'acétate [1-14 C] dans les précurseurs stéroliques (4,4-diméthylstérols et 4␣-méthylstérols) et dans les phospholipides (PL) totaux dans le mycélium extra-racinaire de R. irregularis cultivé en présence d'anthracène. Ces résultats suggèrent un ralentissement des voies de biosynthèse des stérols et des PL chez le CMA traité avec l'anthracène. L'ensemble de nos données permettent de conclure, qu'en plus de la peroxydation lipidique, le ralentissement des voies de biosynthèse des lipides membranaires pourrait expliquer l'inhibition de croissance du CMA cultivé dans un milieu pollué par les HAP. Cette étude contribue, ainsi, à une meilleure compréhension des mécanismes biochimiques impliqués dans l'écotoxicité des HAP vis-à -vis des CMA.Mots-clés : anthracène, champignon mycorhizien arbusculaire, métabolisme lipidique, polluants, hydrocarbures aromatiques polycycliques.

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Section: Discussionsupporting

confidence: 85%

Section: Control Anthracenementioning

confidence: 76%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

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Impact of anthracene on the arbuscular mycorrhizal fungus lipid metabolism

Calonne

Fontaine

DebianeD.

et al. 2014

Botany

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“…Many factors, such as bioavailability of pollutants, environmental conditions (Tang et al, 2012), soil physicochemical properties, population of rhizosphere microorganisms, and plant species affect the rate of phytoremediation (Khoiy and Noori, 2012). Efficacy in phytoremediation processes relies on selection of appropriate plants, and criteria must include plant tolerance to the pollutant (Debiane et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Leucanthemum vulgare lam. crude oil phytoremediation

Noori

Maivan

Alaie³

et al. 2018

International Journal of Phytoremediation

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Sites with crude oil pollution have been successfully treated using phytoremediation, but expanding the range of plants that can be used and understanding how exposure impacts the plants are two areas of study that are important to continue. Leucanthemum vulgare has been shown to grow well under a variety of stressful conditions. To examine L. vulgare's ability to both survive crude oil exposure and to reduce crude oil concentrations in soil, plants were placed in soil containing 0, 2.5%, 5%, 7.5% or 10% w/w crude oil. Total Petroleum Hydrocarbons (TPH) concentration, peroxidase and catalase activity, proline and phenol content in roots and leaves were determined at the start of planting and every 2 months for six months. L. vulgare roots were successfully colonized with mycorrhizae under all conditions. Results showed positive correlation between antioxidant compound concentration and crude oil contamination. Also, a significant reduction occurred in TPH content of soil over time in planted pots as compared to controls. The lowest TPH content was recorded after 6 months under all treatments. Results showed L. vulgare could survive crude oil exposure and enhance reducing of crude oil from soil.

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