Lipid content and fatty acid composition of heart and muscle of the BIO 82.62 cardiomyopathic hamster

Abstract

Microscopic and analytical studies of the lipids in the heart and muscle of the BIO 82.62 myopathic hamsters and age-matched normal animals at the average ages of 33, 67, and 108 days were performed. Microscopic examinations did not show increased lipid depositions in the hearts of the diseased animals as was found in the BIO 14.6 strain. No consistent differences in the lipid content of the cardiomyopathic hamsters (BIO 82.62) and age-matched controls were observed in the three age groups except in the cholesterol content of muscle. Cholesterol increased significantly (P less than 0.01) in the 67 and 108 day old animals. This increase elevated the cholesterol/phospholipid ratio. Analysis of the fatty acid composition of triglycerides showed that the cardiomyopathic hamsters store more saturated fatty acids in both heart and muscle than do their normal counterparts. The abundance of more saturated fatty acids may imply that either the desaturation mechanism is altered in the diseased animals or that unsaturated fatty acids are preferentially utilized in other processes.

Alteration in 1,2-diacylglycerol level and its fatty acid composition in hearts during the growth of hamsters

“…Because of a lack of exercise, the myocardium may come to contain lipid droplets consisting primarily of triglycerides (32). Conflicting results have been reported regarding changes in pbospholipids in normal hamster hearts over the course of growth (3,23). The results by Barakat et al (3) for hamsters up to 110 days old were similar to the results described here.…”

Postnatal anatomical and functional development of the heart: A species comparison

“…In hamsters, heart lipid and phospholipids increase during the first 4 months postnatally (Barakat et al, 1976). source than the adult heart (Rakusan, 1980 for further review).…”

Differential modulation of the functionality of white adipose tissue of obese Zucker (fa/fa) rats by the type of protein and the amount and type of fat

“…Total lipids were extracted according to Folch [23], and the fatty acids were methylated with 2 ml of methanol containing 0.002% butylated hydroxytoluene, 100 μl of toluene and 40 μl of sulfuric acid dissolved in methanol (2%). The samples were incubated at 90°C for 2 h, 1 ml of 5% NaCl was added, and then the methylated fatty acids were extracted with 2 ml of hexane [24]. The organic phase was evaporated under a nitrogen stream until dryness, and the residue fatty acid methyl esters (FAME) were dissolved in 200 μl hexane for analysis by gas chromatography (Agilent 6850 GC with flame ionization detector) using an HP-1 capillary column (30-m × 0.32-mm inner diameter with 0.25-mm film thickness; J&W Scientific, Albany, NY, USA).…”