Lipid composition of thermophilic Geobacillus sp. strain GWE1, isolated from sterilization oven

Shah, Siddharth; Jansen, Susan A.; Taylor, Leeandrew Jacques-Asa; Chong, Parkson Lee‐Gau; Correa-Llantén, Daniela N.; Blamey, Jenny M.

doi:10.1016/j.chemphyslip.2014.02.005

Cited by 6 publications

(4 citation statements)

References 26 publications

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“…2D 13 C- 1 H HSQC NMR was also used for phospholipid profiling of thermophilic Geobacillus sp. strain GWE1 isolated from sterilization ovens, which confirmed the 1 H and 13 C NMR assignments of the glycerol backbone position sn-1 and the CH 2 CH 2 NH 2 head group . However, application of 2D HSQC to lipidomics mixtures for untargeted lipid identification and quantification is still very limited, which is partly due to the lack of curated and comprehensive lipid 2D NMR databases that are coupled with user-friendly platforms for spectral analysis.…”

Section: Introductionmentioning

confidence: 55%

“…Multidimensional high-resolution solution NMR spectroscopy can provide detailed and unique fingerprint information of chemical compounds in complex chemical mixtures without the need for extensive sample purification/separation. − Two-dimensional (2D) 13 C- 1 H heteronuclear single quantum coherence (HSQC) has been applied for lipid profiling in a variety of lipid cell extracts. − Mycobacterial cell wall lipids were analyzed by 2D 13 C- 1 H HSQC to monitor unique biomarker cross-peaks and test for the presence of certain lipid species to understand the role of lipids in mycobacteria pathogenesis . 2D 13 C- 1 H HSQC NMR was also used for phospholipid profiling of thermophilic Geobacillus sp.…”

Section: Introductionmentioning

confidence: 99%

“…strain GWE1 isolated from sterilization ovens, which confirmed the 1 H and 13 C NMR assignments of the glycerol backbone position sn-1 17 and the CH 2 CH 2 NH 2 head group. 16 However, application of 2D HSQC to lipidomics mixtures for untargeted lipid identification and quantification is still very limited, which is partly due to the lack of curated and comprehensive lipid 2D NMR databases that are coupled with user-friendly platforms for spectral analysis. Moreover, certain lipids within the same lipid class share common substructures that tend to give rise to similar chemical shifts, requiring NMR spectra with very high resolution for their unambiguous discrimination (see Supporting Information, Figure S1).…”

Section: ■ Introductionmentioning

confidence: 99%

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COLMAR Lipids Web Server and Ultrahigh-Resolution Methods for Two-Dimensional Nuclear Magnetic Resonance- and Mass Spectrometry-Based Lipidomics

et al. 2020

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Accurate identification of lipids in biological samples is a key step in lipidomics studies. Multidimensional nuclear magnetic resonance (NMR) spectroscopy is a powerful analytical tool for this purpose as it provides comprehensive structural information on lipid composition at atomic resolution. However, the interpretation of NMR spectra of complex lipid mixtures is currently hampered by limited spectral resolution and the absence of a customized lipid NMR database along with user-friendly spectral analysis tools. We introduce a new two-dimensional (2D) NMR metabolite database “COLMAR Lipids” that was specifically curated for hydrophobic metabolites presently containing 501 compounds with accurate experimental 2D 13C-1H heteronuclear single quantum coherence (HSQC) chemical shift data measured in CDCl3. A new module in the public COLMAR suite of NMR web servers was developed for the (semi)automated analysis of complex lipidomics mixtures (). To obtain 2D HSQC spectra with the necessary high spectral resolution along both 13C and 1H dimensions, nonuniform sampling in combination with pure shift spectroscopy was applied allowing the extraction of an abundance of unique cross-peaks belonging to hydrophobic compounds in complex lipidomics mixtures. As shown here, this information is critical for the unambiguous identification of underlying lipid molecules by means of the new COLMAR Lipids web server, also in combination with mass spectrometry, as is demonstrated for Caco-2 cell and lung tissue cell extracts.

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Section: Introductionmentioning

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

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COLMAR Lipids Web Server and Ultrahigh-Resolution Methods for Two-Dimensional Nuclear Magnetic Resonance- and Mass Spectrometry-Based Lipidomics

et al. 2020

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“…In addition to the unsaturation of fatty acids in the carbonyl region, the absorption band C = C (1650 cm -1 ) can also be observed in some samples. The absorption between 1477–1452 cm -1 and 725 cm-1 may be due to the asymmetric stretching and deformation of the C-H of methylene groups of the fatty acids, which are characteristic of long chain hydrocarbons containing (-CH 2 -)n. The 1380 to 1350 cm -1 region is the -CH 3 group indicative of the isopropyl group [ 35 ]. At approximately 1229 cm -1 lies the C-O group stretching vibration region of esters [ 36 ].…”

Section: Resultsmentioning

confidence: 99%

Bacillus megaterium strains derived from water and soil exhibit differential responses to the herbicide mesotrione

Dobrzanski¹,

Gravina²,

Steckling³

et al. 2018

PLoS ONE

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The intense use of herbicides for weed control in agriculture causes selection pressure on soil microbiota and water ecosystems, possibly resulting in changes to microbial processes, such as biogeochemical cycles. These xenobiotics may increase the production of reactive oxygen species and consequently affect the survival of microorganisms, which need to develop strategies to adapt to these conditions and maintain their ecological functionality. This study analyzed the adaptive responses of bacterial isolates belonging to the same species, originating from two different environments (water and soil), and subjected to selection pressure by herbicides. The effects of herbicide Callisto and its active ingredient, mesotrione, induced different adaptation strategies on the cellular, enzymatic, and structural systems of two Bacillus megaterium isolates obtained from these environments. The lipid saturation patterns observed may have affected membrane permeability in response to this herbicide. Moreover, this may have led to different levels of responses involving superoxide dismutase and catalase activities, and enzyme polymorphisms. Due to these response systems, the strain isolated from water exhibited higher growth rates than did the soil strain, in evaluations made in oligotrophic culture media, which would be more like that found in semi-pristine aquatic environments. The influence of the intracellular oxidizing environments, which changed the mode of degradation of mesotrione in our experimental model and produced different metabolites, can also be observed in soil and water at sites related to agriculture. Since the different metabolites may present different levels of toxicity, we suggest that this fact should be considered in studies on the fate of agrochemicals in different environments.

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Lipases/esterases from extremophiles: main features and potential biotechnological applications

Luca

Mandrich

2020

Physiological and Biotechnological Aspects of Extremophiles

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Lipid composition of thermophilic Geobacillus sp. strain GWE1, isolated from sterilization oven

Cited by 6 publications

References 26 publications

COLMAR Lipids Web Server and Ultrahigh-Resolution Methods for Two-Dimensional Nuclear Magnetic Resonance- and Mass Spectrometry-Based Lipidomics

COLMAR Lipids Web Server and Ultrahigh-Resolution Methods for Two-Dimensional Nuclear Magnetic Resonance- and Mass Spectrometry-Based Lipidomics

Bacillus megaterium strains derived from water and soil exhibit differential responses to the herbicide mesotrione

Lipases/esterases from extremophiles: main features and potential biotechnological applications

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