Lipid composition of the synaptosome and erythrocyte membranes during chronic ethanol-treatment and withdrawal in the rat

Droitte, Philippe La; Lamboeuf, Y; Saint-Blanquat, G. De

doi:10.1016/0006-2952(84)90317-4

Cited by 40 publications

(5 citation statements)

References 19 publications

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“…Data concerning these changes are very heterogeneous, depending on the model of ethanol intoxication and on the organ studied. In brain, unsaturation index was found decreased [46][47][48] or increased [49]. In the liver, data were also conflicting [10,50,51].…”

Section: Discussionmentioning

confidence: 83%

Polyunsaturated fatty acid deficiency reverses effects of alcohol on mitochondrial energy metabolism

Piquet

Roulet

Nogueira

et al. 2004

Journal of Hepatology

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Section: Discussionmentioning

confidence: 83%

Polyunsaturated fatty acid deficiency reverses effects of alcohol on mitochondrial energy metabolism

Piquet

Roulet

Nogueira

et al. 2004

Journal of Hepatology

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“…In addition to this, ethanol has several effects on synaptic structures of brain such as altering lipid structure and composition of synaptic membranes [9,10], decreasing the number of synaptic vesicles (SV) while increasing intramembranous particles [11] and inhibiting plasma membrane and mitochondrial transmembrane potentials [12]. Acetaldehyde also decreases the viscosity of hydrophobic regions of bulk membrane lipids [13].…”

Section: Introductionmentioning

confidence: 99%

Effects of Chronic Ethanol Consumption on Brain Synaptosomes and Protective Role of Betaine

et al. 2007

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To evaluate the cytotoxic effects of chronic ethanol consumption on brain cerebral synaptosomes and preventive role of betaine as a methyl donor and S-adenosylmethionine precursor, 24 male Wistar rats were divided into three groups: control, ethanol (8 g/kg/day) and ethanol plus betaine(0.5% w/v) group. Animals were fed 60 ml/diet per day for two months, then sacrificed. Malondialdehyde (MDA), protein carbonyl contents and adenosine deaminase (ADA) activities were determined in synaptosomal/mitochondrial enriched fraction isolated from rat cerebral cortexes. When compared to controls, ethanol containing diet significantly increased MDA levels (P < 0.05), also increased protein carbonyl levels and adenosine deaminase activities. But these were not statistically significant (P > 0.05). However, adding betaine to ethanol containing diet caused a significant decrease in MDA, protein carbonyl levels and adenosine deaminase activities (P < 0.05). These results indicate that betaine may appear as a protective nutritional agent against cytotoxic brain damage induced by chronic ethanol consumption.

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“…At anaesthetic concentrations of anaesthetic agents, no detectable changes in the lipid composition or fluidity of neural cell membranes have been detected (Franks & Lieb 1978) when acute exposures resembling clinical anaesthesia have been used. In chronic exposures, the lipid structure of the membrane is affected, too (la Droitte et al 1984;Kyrklund et al 1987). In our previous studies, we have found that aromatic hydrocarbons have an inhibitory effect on both erythrocyte and synaptosome membrane-bound enzymes AChE and ATPase in in vitro conditions (Korpela, unpublished results;Korpela & Tihti 1986.…”

mentioning

confidence: 95%

“…The main factor determining this binding is the lipid solubility of compounds, but the structure and size of the anaesthetic molecule are also of importance. In chronic exposures, the lipid structure of the membrane is affected, too (la Droitte et al 1984;Kyrklund et al 1987). In chronic exposures, the lipid structure of the membrane is affected, too (la Droitte et al 1984;Kyrklund et al 1987).…”

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confidence: 99%

The Effect of in Vitro and in Vivo Toluene Exposure on Rat Erythrocyte and Synaptosome Membrane Integral Enzymes

Korpela¹,

Tähti²

1988

Pharmacology & Toxicology

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The mechanism of the anaesthetic effect of toluene on the central nervous system (CNS) was studied by using rat erythrocyte and synaptosome membranes as nerve cell models both in vitro and in vivo. The activities of the membrane-bound integral enzymes acetylcholinesterase (AChE), total adenosine triphosphatase (total ATPase) and magnesium-activated adenosine triphosphatase (Mg2+-ATPase) were determined. A short-term exposure to 2000 p.p.m. of toluene had an inhibitory effect on the enzyme activities studied. The degree of inhibition in erythrocyte membranes in vitro and in vivo, and in synaptosome membranes in vitro were in good correlation. In in vivo conditions, the synaptosome-bound enzymes were, however, significantly more inhibited by toluene, which indicates that membranes in vivo are even more vulnerable to the toxic effects of organic solvents than they are as isolated membranes in vitro. However, our results show that in vitro experiments can be used to predict the toxic nerve cell membrane effects of organic solvents. Toluene caused similar enzyme inhibitions both in neural cell membranes and in erythrocyte membranes. Thus, even peripheral non-excitable cell membranes, like erythrocytes, can be used as nerve cell membrane models in studies on the mechanism of the anaesthesia caused by solvents.

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Lipid composition of the synaptosome and erythrocyte membranes during chronic ethanol-treatment and withdrawal in the rat

Cited by 40 publications

References 19 publications

Polyunsaturated fatty acid deficiency reverses effects of alcohol on mitochondrial energy metabolism

Polyunsaturated fatty acid deficiency reverses effects of alcohol on mitochondrial energy metabolism

Effects of Chronic Ethanol Consumption on Brain Synaptosomes and Protective Role of Betaine

The Effect of in Vitro and in Vivo Toluene Exposure on Rat Erythrocyte and Synaptosome Membrane Integral Enzymes

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