Lipid Composition of Human Aqueous Humor

Jahn, Christine; Leiß, O.; Bergmann, Klaus von

doi:10.1159/000265263

Cited by 17 publications

(10 citation statements)

References 6 publications

(10 reference statements)

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“…In bovine (table 3) as well as in human serum the FA-toalbumin ratios are normally below 1. Therefore, the ratios calculated for human AH, using the FA concentrations determined by Jahn et al [12], seem to be rather high. Although we find clearly lower ratios in bovine AH, our results also demonstrate higher FA-to-albumin ratios in AH than in serum.…”

Section: Discussionmentioning

confidence: 86%

“…However, to our knowledge no data are available in the literature for that ratio in AH, so far. Using lipid extraction, thin-layer chromatography and densitometry, Jahn et al [12] investigated lipid components in AH from 10 patients undergoing cataract surgery. For nonesterified FAs they found values between 0.2 and 2.9 mg/100 ml with a mean of 1.1 mg/100 ml.…”

Section: Discussionmentioning

confidence: 99%

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Concentrations of Serum Albumin and Nonesterified Fatty Acids in Bovine Aqueous Humor with Regard to the Fatty Acid Sensitivity of Bovine Lens Epithelial Cells

John

Glaesser²

2000

Ophthalmic Res

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Concentrations of albumin and nonesterified fatty acids (FAs) have been determined in bovine serum and aqueous humor (AH) with regard to the FA-to-albumin molar ratio, which has been proved decisive for the FA cytotoxicity to bovine lens epithelial cells as described previously. In 6- to 7-month-old female calves the average albumin level was reduced 309-fold from 638 µmol/l in serum to 2.00 µmol/l in AH, in 72- to 84-month-old cows 405-fold from 632 to 1.56 µmol/l, and in 12- to 18-month-old bulls 395-fold from 553 to 1.40 µmol/l. In 64% of the animals the albumin concentration in AH differed significantly between both eyes. The serum albumin values resulted in a Gaussian curve, but the frequency distribution for AH albumin values skewed to the right. Using an enzymatic method the concentrations of nonesterified FAs were determined in serum as well as in concentrated AH samples. In all animals investigated the molar FA-to-albumin ratio was higher in AH than in serum. The results are discussed in view of the assumption that unsaturated nonesterified FAs might be cataractogenic risk factors.

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Concentrations of Serum Albumin and Nonesterified Fatty Acids in Bovine Aqueous Humor with Regard to the Fatty Acid Sensitivity of Bovine Lens Epithelial Cells

John

Glaesser²

2000

Ophthalmic Res

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“…The AH is composed of ions (Na + , K + , Ca 2+ , Mg 2+ , Cl -, HCO 3 -), crystalloid or low molecular weight substances (glucose, ascorbate, lactate, pyruvate, urea, H2O2, amino acids, etc. ), colloidal or high molecular weight substances, such as proteins (Borazan et al, 2010;Browne et al, 2011;Ghanem et al, 2011;Lim et al, 2010), lipids (Jahn et al, 1983), biologically active substances (catecholamines, eicosanoids, hormones, etc.) as well as some miscellaneous substances such as hyaluronic acid, hyaluronidase, etc.).…”

Section: Composition Of the Aqueous Humormentioning

confidence: 99%

Mechanism of Aqueous Humor Secretion, Its Regulation and Relevance to Glaucoma

Shahidullah¹,

Almalki²,

Delamere³

2011

Glaucoma - Basic and Clinical Concepts

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“…In this respect, the lipid signalling provides greater efficiency compared to that by proteins. Normal AH has been shown to contain diverse phospholipids (PLs) . The TM and AH phospholipids and sphingolipids of human control and POAG as well as normotensive and ocular hypertensive DBA/2J mouse have been previously analysed using moderate‐resolution mass spectrometers …”

Section: Introductionmentioning

confidence: 99%

Endogenous ocular lipids as potential modulators of intraocular pressure

Edwards

Arcuri

Wang

et al. 2020

J Cellular Molecular Medi

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Elevated intraocular pressure (IOP) is a risk factor in glaucoma, a group of irreversible blinding diseases. Endogenous lipids may be involved in regulation of IOP homeostasis. We present comparative fold analysis of phospholipids and sphingolipids of aqueous humour and trabecular meshwork from human control vs primary openangle glaucoma and mouse control (normotensive) vs ocular hypertensive state. The fold analysis in control vs disease state was based on ratiometric mass spectrometric data for above classes of lipids. We standardized in vitro assays for rapid characterization of lipids undergoing significant diminishment in disease state. Evaluation of lipids using in vitro assays helped select a finite number of lipids that may potentially expand cellular interstitial space embedded in an artificial matrix or increase fluid flow across a layer of cells. These assays reduced a number of lipids for initial evaluation using a mouse model, DBA/2J with spontaneous IOP elevation. These lipids were then used in other mouse models for confirmation of IOP lowering potential of a few lipids that were found promising in previous assessments. Our results provide selected lipid molecules that can be pursued for further evaluation and studies that may provide insight into their function. K E Y W O R D Saqueous humour, glaucoma, lipidomics, lipids, trabecular meshwork | 3857 EDWARDS Et Al.

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Lipid Composition of Human Aqueous Humor

Cited by 17 publications

References 6 publications

Concentrations of Serum Albumin and Nonesterified Fatty Acids in Bovine Aqueous Humor with Regard to the Fatty Acid Sensitivity of Bovine Lens Epithelial Cells

Concentrations of Serum Albumin and Nonesterified Fatty Acids in Bovine Aqueous Humor with Regard to the Fatty Acid Sensitivity of Bovine Lens Epithelial Cells

Mechanism of Aqueous Humor Secretion, Its Regulation and Relevance to Glaucoma

Endogenous ocular lipids as potential modulators of intraocular pressure

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