“…According to the previously described procedure (Yoshida, Tomiyama, & Mizushina, 2010), total lipids were separated by TLC into eight sufractions with a solvent system of n-hexane/diethyl ether/acetic acid (80:20:1, v/v/v). Bands corresponding to HC, SE, TAG, FFA, 1,3-DAG, 1,2-DAG, unknown and PL were scraped into separate test-tubes [105 x 16 mm; poly (tetrafluoroethylene)-coated screw caps].…”