Lipid‐Coated Microbubbles: Enhanced Osteogenic Differentiation of Human Mesenchymal Stem Cells Using Microbubbles and Low Intensity Pulsed Ultrasound on 3D Printed Scaffolds (Adv. Biosys. 2/2019)

Osborn, Jenna; Aliabouzar, Mitra; Zhou, Xuan; Rao, Raj D.; Zhang, Lijie Grace; Sarkar, Kausik

doi:10.1002/adbi.201970021

Cited by 2 publications

(3 citation statements)

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“…This strategy of LIPUS along with microbubbles can be used for bone tissue engineering applications in the future. 129 Migration of BMSCs by LIPUS has been studied further in vitro and in vivo. Also, the underlying mechanism involving FAK and ERK1/2 signaling pathways are also investigated.…”

Section: Applications Of Ultrasound In Stem Cellsmentioning

confidence: 99%

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Ultrasound-Enabled Therapeutic Delivery and Regenerative Medicine: Physical and Biological Perspectives

Bansal

Jha

Bhatia

et al. 2021

ACS Biomater. Sci. Eng.

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The role of ultrasound in medicine and biological sciences is expanding rapidly beyond its use in conventional diagnostic imaging. Numerous studies have reported the effects of ultrasound on cellular and tissue physiology. Advances in instrumentation and electronics have enabled successful in vivo applications of therapeutic ultrasound. Despite path breaking advances in understanding the biophysical and biological mechanisms at both microscopic and macroscopic scales, there remain substantial gaps. With the progression of research in this area, it is important to take stock of the current understanding of the field and to highlight important areas for future work. We present herein key developments in the biological applications of ultrasound especially in the context of nanoparticle delivery, drug delivery, and regenerative medicine. We conclude with a brief perspective on the current promise, limitations, and future directions for interfacing ultrasound technology with biological systems, which could provide guidance for future investigations in this interdisciplinary area.

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Section: Applications Of Ultrasound In Stem Cellsmentioning

confidence: 99%

“…Total calcium deposition and ALP activity had similar patterns. This strategy of LIPUS along with microbubbles can be used for bone tissue engineering applications in the future …”

Section: Applications Of Ultrasound In Stem Cells Engineering and Reg...mentioning

confidence: 99%

Ultrasound-Enabled Therapeutic Delivery and Regenerative Medicine: Physical and Biological Perspectives

Bansal

Jha

Bhatia

et al. 2021

ACS Biomater. Sci. Eng.

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“…Tissue-engineering technologies provide valuable artificial replacement and implants for tissue restructure and regeneration by combining engineered scaffolds and stem cells. However, traditional tissue engineering faces some restrictions, including low precision and resolution in replicating biomimetic architectures, an inability to create a complex hierarchical structure, and multistep fabrication procedures. , Recently, traditional tissue-engineering technologies have been revolutionized and reformed by the emergence of state-of-the-art 3D bioprinting technologies, which can print customizable hierarchical structures with high precision and high efficiency by concise procedures. ,− For example, stereolithography (SL)-based printing techniques utilize a rapid lithographic methodology to photo-cross-link photopolymer inks in a layer-by-layer fashion using a photochemical process. , The photopolymer ink can be customized and formulated to have either a single component or multiple components, such as biomacromolecules, nanocomposites, polymers, and even live cells, which can be used in various applications. Therefore, the SL printing technique provides a versatile 3D printing platform for tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Printing Biologically Inspired DNA-Based Gradient Scaffolds for Cartilage Tissue Regeneration

Zhou

Tenaglio

Esworthy

et al. 2020

ACS Appl. Mater. Interfaces

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Cartilage damage caused by aging, repeated overloading, trauma, and diseases can result in chronic pain, inflammation, stiffness, and even disability. Unlike other types of tissues (bone, skin, muscle, etc.), cartilage tissue has an extremely weak regenerative capacity. Currently, the gold standard surgical treatment for repairing cartilage damage includes autografts and allografts. However, these procedures are limited by insufficient donor sources and the potential for immunological rejection. After years of development, engineered tissue now provides a valuable artificial replacement for tissue regeneration purposes. Threedimensional (3D) bioprinting technologies can print customizable hierarchical structures with cells. The objective of the current work was to prepare a 3D-printed three-layer gradient scaffold with lysine-functionalized rosette nanotubes (RNTK) for improving the chondrogenic differentiation of adipose-derived mesenchymal stem cells (ADSCs). Specifically, biologically inspired RNTKs were utilized in our work because they have unique surface chemistry and biomimetic nanostructure to improve cell adhesion and growth. Different ratios of gelatin methacrylate (GelMA) and poly(ethylene glycol) diacrylate (PEGDA) were printed into a three-layer GelMA−PEGDA gradient scaffold using a stereolithography-based printer, followed by coating with RNTKs. The pores and channels (∼500 μm) were observed in the scaffold. It was found that the population of ADSCs on the GelMA−PEGDA−RNTK scaffold increased by 34% compared to the GelMA−PEGDA scaffold (control). Moreover, after 3 weeks of chondrogenic differentiation, collagen II, glycosaminoglycan, and total collagen synthesis on the GelMA−PEGDA−RNTK scaffold significantly respectively increased by 59%, 71%, and 60%, as compared to the control scaffold. Gene expression of collagen II α1, SOX 9, and aggrecan in the ADSCs growing on the GelMA− PEGDA−RNTK scaffold increased by 79%, 52%, and 47% after 3 weeks, compared to the controls, respectively. These results indicated that RNTKs are a promising type of nanotubes for promoting chondrogenic differentiation, and the present 3D-printed three-layer gradient GelMA−PEGDA−RNTK scaffold shows considerable promise for future cartilage repair and regeneration.

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Lipid‐Coated Microbubbles: Enhanced Osteogenic Differentiation of Human Mesenchymal Stem Cells Using Microbubbles and Low Intensity Pulsed Ultrasound on 3D Printed Scaffolds (Adv. Biosys. 2/2019)

Cited by 2 publications

References 0 publications

Ultrasound-Enabled Therapeutic Delivery and Regenerative Medicine: Physical and Biological Perspectives

Ultrasound-Enabled Therapeutic Delivery and Regenerative Medicine: Physical and Biological Perspectives

Three-Dimensional Printing Biologically Inspired DNA-Based Gradient Scaffolds for Cartilage Tissue Regeneration

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