Lipid class and fatty acid distributions in particulate and dissolved fractions in the north Adriatic sea

Derieux, S.; Fillaux, Joëlle; Saliot, A.

doi:10.1016/s0146-6380(98)00089-8

Cited by 53 publications

(24 citation statements)

References 29 publications

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“…Since lipid classes are useful markers to determine sources and fate of organic matter in marine systems (Parrish, 1988) they have widely been used as biological tracers (Parrish et al, 1995;Derieux et al, 1998;Goutx et al, 2000), but only once in conjunction with bacterial community structure changes (Ghiglione et al, 2007). In the present study CCA analysis showed that dissolved lipids from chloroplast origin (LC), which indicate phytoplankton source of DOM (Goutx et al, 1990), were the main biotracer associated with bacterial community structure, due surely to their high concentration at particular samples of 5-20 m depths (Fig.…”

Section: Cca With Lipid Class Biotracersmentioning

confidence: 99%

Role of environmental factors for the vertical distribution (0–1000 m) of marine bacterial communities in the NW Mediterranean Sea

et al. 2008

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Abstract. Bacterioplankton plays a central role in energy and matter fluxes in the sea, yet the factors that constrain its variation in marine systems are still poorly understood. Here we use the explanatory power of direct multivariate gradient analysis to evaluate the driving forces exerted by environmental parameters on bacterial community distribution in the water column. We gathered and analysed data from a one month sampling period from the surface to 1000 m depth at the JGOFS-DYFAMED station (NW Mediterranean Sea). This station is characterized by very poor horizontal advection currents which makes it an ideal model to test hypotheses on the causes of vertical stratification of bacterial communities. Capillary electrophoresis single strand conformation polymorphism (CE-SSCP) fingerprinting profiles analyzed using multivariate statistical methods demonstrated a vertical zonation of bacterial assemblages in three layers, above, in or just below the chlorophyll maximum and deeper, that remained stable during the entire sampling period. Through the use of direct gradient multivariate ordination analyses we demonstrate that a complex array of biogeochemical parameters is the driving force behind bacterial community structure shifts in the water column. PhysicoCorrespondence to: J. F. Ghiglione (ghiglione@obs-banyuls.fr) chemical parameters such as phosphate, nitrate, salinity and to a lesser extent temperature, oxygen, dissolved organic carbon and photosynthetically active radiation acted in synergy to explain bacterial assemblages changes with depth. Analysis of lipid biomarkers of organic matter sources and fates suggested that bacterial community structure in the surface layers was in part explained by lipids of chloroplast origin. Further detailed analysis of pigment-based phytoplankton diversity gave evidence of a compartmentalized influence of several phytoplankton groups on bacterial community structure in the first 150 m depth.

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Section: Cca With Lipid Class Biotracersmentioning

confidence: 99%

Role of environmental factors for the vertical distribution (0–1000 m) of marine bacterial communities in the NW Mediterranean Sea

et al. 2008

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“…Lipids can be used as source indicators through investigation of various marker series (28) or by considering the relative abundance of lipid classes (20,27,29). Lipid classes reveal contributions from living organisms (PL), metabolic reserves (TG) and breakdown products from freshly synthesized OM (MG, DG and FFA).…”

Section: Lipid Analysismentioning

confidence: 99%

“…Total lipid extracts were analyzed for lipid classes by thin layer chromatography with the flame ionization detection (TLC-FID) Iatroscan technique, sensitive and qualitative for each lipid class with internal calibration (20,26).…”

Section: Lipid Analysismentioning

confidence: 99%

“…Direct measurements in samples without any pretreatment (DOC and electrochemical measurements) provided information about the natural concentration level of OM and the characteristics of these substances. Lipids were estimated from extracted films (4,20) while, for monolayer studies and BAM measurements, model films ex-situ reconstructed from extracted dissolved lipids of sea-surface microlayer samples were used. The ultimate aim was the physico-chemical characterization of natural sea-surface microlayer samples, comparing the results obtained by different methods.…”

Section: Introductionmentioning

confidence: 99%

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Physicochemical Characterization of Natural andex-SituReconstructed Sea-Surface Microlayers

Gašparović

Kozarac

Saliot

et al. 1998

Journal of Colloid and Interface Science

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Running title: Sea-surface microlayers 2 Chemical composition and physico-chemical characteristics of natural and ex-situ reconstructed sea surface microlayer samples were studied using a complex methodological approach. Surface microlayer samples and the underlying seawater were collected in different seasons and different weather conditions in the northern most part of the Adriatic sea. The techniques used were thin layer chromatography with flame ionization detection for lipid classes analysis, electrochemical characterization of adsorbable organic substances using the ONP probe and monolayer techniques for surface pressure () and surface potential ( V) measurements, as well as Brewster angle microscopy.Our results indicated higher enrichment of organic matter for the microlayer sample collected in the warm period of the year (summer). This was reflected in the high surface activity of the present organic substances, and formation of a more condensed layer than for the other two samples collected in the spring. Of the two spring samples, the one sampled on a sunny day was reacher in surface active organic material. We concluded that the most important source of surface active substances is the insitu production of organic susbtances by the present plankton and bacteria, promoted by sunlight, while antrophogenic input comprises a smaller part of the present organic matter, around 10 % for all samples.Key Words: sea-surface microlayer; lipid analysis; o-nitrophenol; surface active organic substances; voltammetry; monolayer studies; Brewster angle microscopy; northern Adriatic. 3 INTRODUCTIONSea surface microlayer is an important boundary, as an area of exchange of matter and energy, that either affects or is affected by global change (1, 2). Sea surface layer is generally enriched in organic substances, particularly those which are surface active, metal ions, bacteria and other microorganisms, relative to subsurface water (3-5). Organic substances found in the microlayer originate from the in-situ production or from different sources and have been brought there by different transport mechanisms from the seawater bulk, atmosphere or land. Adsorbed organic substances change the physical and optical properties, depending on the nature of organics, i.e. the nature of polar groups, architecture of the hydrophobic chain, and ionic strength, pH and temperature.Although the physical and chemical properties of the sea-surface microlayer have been studied extensively, there is still lack of knowledge about the physico-chemical processes governing the formation and properties of the surface microlayer.Electrochemical methods have been widely used for the investigation of surface active substances (SAS) in different waters (6 -9). These methods are based on the principle that organic substances adsorbed on the electrode surface influence the mass and charge transport across such a modified phase boundary. The recently developed electrochemical method using the o-nitrophenol (ONP) probe as a convenient tool for rough char...

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“…With deceasing in proportions of some saturated and monounsaturated fatty acids (e.g., 14:0 and 16:0, 16:1), the bacteria-specific fatty acids such as iso-and anteiso-15:0 and 17:0, and 18:1(n7) increased their proportions in deep waters. Many previous studies [17][18][19] have demonstrated that fatty acids are a dominant lipid component in marine POM, DOM, and colloidal OM. Their relative compositions in these OM pools varied with primary production, microbial activity, and environmental conditions.…”

Section: Resultsmentioning

confidence: 99%

Origins of High-Molecular-Weight Dissolved Organic Matter in the Middle Atlantic Bight: Clue from Lipids and Molecular δ13C Ratios

Zou¹,

Guo²,

Sun³

et al. 2008

TOOCEAJ

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Lipid class and fatty acid distributions in particulate and dissolved fractions in the north Adriatic sea

Cited by 53 publications

References 29 publications

Role of environmental factors for the vertical distribution (0–1000 m) of marine bacterial communities in the NW Mediterranean Sea

Role of environmental factors for the vertical distribution (0–1000 m) of marine bacterial communities in the NW Mediterranean Sea

Physicochemical Characterization of Natural andex-SituReconstructed Sea-Surface Microlayers

Origins of High-Molecular-Weight Dissolved Organic Matter in the Middle Atlantic Bight: Clue from Lipids and Molecular δ13C Ratios

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