Lipid characteristics of RSV‐transformed Balb/c 3T3 cell lines with different spontaneous metastatic potentials

Abstract

To determine whether a metastatic phenotype may be correlated with a characteristic lipid pattern, we compared the lipid composition of low metastasizing Balb/c 3T3 cells transformed by the B77 strain of Rous sarcoma virus (B77-3T3 cells) with that of a subclone isolated by growth in 0.6% agar, the B77-AA6 cells, which exhibit a high capacity for spontaneous metastasis. B77-3T3 cells revealed characteristics in their lipid composition common to other systems of transformed cells, i.e., an accumulation of ether-linked lipids, a reduction of the more complex gangliosides, an increase of oleic acid (18:1) and a decrease of arachidonic (20:4) and C22 polyunsaturated fatty acids in phospholipids. High metastatic B77-AA6 cells showed: a) an even more marked decrease of complex gangliosides; b) a more pronounced increase of 18:1 and decrease of 20:4 and 22 polyunsaturated fatty acids in certain phospholipid classes; and c) a higher percentage of alkyl-acyl subfractions in both phosphatidylcholine and phosphatidylethanolamine than B77-3T3 cells. Comparing the data for other systems of metastatic cells with those of lipid studies of spontaneously metastasizing B77-AA6 cell system leads us to conclude that the metastatic phenotype is characterized by a change in ether-linked lipids, rather than in fatty acids.

Assessment of the Molecular Expression and Structure of Gangliosides in Brain Metastasis of Lung Adenocarcinoma by an Advanced Approach Based on Fully Automated Chip-Nanoelectrospray Mass Spectrometry

“…Thus, the relative abundances of GM3 and GM2 fractions were 22.5-and 5.5-folds higher, whereas GM1, GD1b, and GT1b fractions showed 25.5-, 17.0-, and 2.1-fold lower relative abundances. These results are consistent with literature data showing that GM3 was the main ganglioside component in all metastatic tumors of human brain of various origins and that the reduced content of complex gangliosides, i.e., GM1, GD1b, GD1a, and GT1b is common for many types of transformed cells, especially for metastatic tumor cells [56][57][58][59][60][61].…”

Macrophage populations of different origins have distinct susceptibilities to lipid peroxidation induced by β‐haematin (malaria pigment)

“…The membranes of microglia and J774 cells are rich in oleic acid (C18:1) and low in linoleic (C18:2 n-6) and arachidonic acid (C20:4 n-6) ( Table 1). This pattern is similar to that reported for other types of tumour cells [30] macrophages, that are richer in C18:2 n-6 and C20:4 n-6 PUFA. Fatty acid composition of peritoneal macrophage accounts for the higher DBI compared to microglia and J774 cells.…”

Composition of ether‐linked sub‐classes of glycerophospholipids in clones with a different metastatic potential isolated from a murine fibrosarcoma line (T3 cells)

“…There are only a few investigations that correlate the level of ether-linked lipids with specific biological parameters of malignant cells, including growth rate (Howard et al, 1972), tumorigenicity (Roos and Choppin, 1984), cellular differentiation (Fallani et al, 1988) and drug resistance (May et al, 1988). Metastatic potential has also been associated with the ether-linked lipid pattern in different systems of metastatic cells, eg., murine mammary carcinomas (Friedberg et al, 1986), F1 and F10 variants of the B16 murine melanoma (Calorini et aL, 1987), and a highly metastatic clone isolated from BALB/c-3T3 cells transformed by RSV (Calorini et al, 1989).In the present study, we investigated whether a specific ether-linked lipid pattern might be correlated with the metastatic potential in a series of clones isolated from T3 cells, a strongly metastatic line derived from the CBAT-3 fibrosarcoma of CBA mice (Bomford and Olivotto, 1974). The system used was developed in our laboratory by growth in soft agar or on plastic (Mannori et al, 1990), a procedure which, unlike isolation under strong selective pressurc, enables us to define variants with biochemical characteristics related to their metastatic capacity.…”

“…However, before we can unambiguously ascribe biological significance to such changes we must first demonstrate that they occur consistently in different systems of metastatic cells. One change that has met this requirement is the accumulation of alkylacyl glycerolipids; it has been observed in a series of metastatic mammary carcinomas (Friedberg et al, 1986), and in strongly metastatic lines such as the F10 line of the B16 murine melanoma (Calorini et al, 1987) and B77-AA6 clone selected from B77-3T3 cells (Calorini et al, 1989).In our system of T3 clones with different metastatic potentials, we found a close correlation between the level of alkylacyl and alkenylacyl glycerolipids and metastatic potential as measured by number of lung nodules (correlation coefficient 0.92 for both sub-fractions). This correlation is closer than that reported for other systems of metastatic cells (Friedberg et al, 1986;Calorini et al, 1987Calorini et al, , 1989.…”