Lipid-based nanoparticles with high binding affinity for amyloid-β1–42 peptide

Gobbi, Marco; Re, Francesca; Canovi, Mara; Beeg, Marten; Gregori, Maria; Sesana, Silvia; Sonnino, Sandro; Brogioli, Doriano; Musicanti, Claudia; Gasco, Paolo; Salmona, Mario; Masserini, Massimo

doi:10.1016/j.biomaterials.2010.04.044

Cited by 187 publications

(172 citation statements)

References 55 publications

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“…16,17 More recently, they also have been applied in real-time live-cell monitoring of a number of cell parameters such as intracellular oxygen concentration. 18 NPs can be made of different inorganic and organic materials: they can be lipid-based (such as liposomes, micelles, solid lipid, or lipoprotein-based NPs), [18][19][20][21] polymeric (such as polylactic acid, poly[lactic-co-glycolic acid], and poly[alkylcyanoacrylate]), 22,23 chitosan-based, 24 quantum dots, 25 silicon-based 26 gold NPs, 27 and magnetic NPs. 17 Since the intrinsic characteristics and thus the relevant applications of NPs are closely related to their size, shape, and surface properties, great efforts have been devoted to control the synthesis of NPs.…”

Section: Why Should Nanoparticles Enter Cells?mentioning

confidence: 99%

The effect of nanoparticle uptake on cellular behavior: disrupting or enabling functions?

Panariti¹,

Miserocchi²,

Rivolta³

2012

NSA

153

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Nanoparticles (NPs) are materials with overall dimensions in the nanoscale range. They have unique physicochemical properties, and have emerged as important players in current research in modern medicine. In the last few decades, several types of NPs and microparticles have been synthesized and proposed for use as contrast agents for diagnostics and imaging and for drug delivery; for example, in cancer therapy. Yet specific targeting that will improve their delivery still represents an unsolved challenge. The mechanism by which NPs enter the cell has important implications not only for their fate but also for their impact on biological systems. Several papers in the literature discuss the potential risks related to NP exposure, and more recently the concept that even sublethal doses of NPs may elicit a cell response has been proposed. In this review, we intend to present an overall view of cell mechanisms that may be perturbed by cell-NP interaction. Published data, in fact, emphasize that NPs should no longer be viewed only as simple carriers for biomedical applications, but that they can also play an active role in mediating biological effects.

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Section: Why Should Nanoparticles Enter Cells?mentioning

confidence: 99%

The effect of nanoparticle uptake on cellular behavior: disrupting or enabling functions?

Panariti¹,

Miserocchi²,

Rivolta³

2012

NSA

153

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“…For uptake and permeability studies, 0.001 molar % of [ 3 H]-Sm was added as tracer to follow lipid distribution by radioactivity counting. NL was prepared in 10 mM PBS, pH 7.4 by extrusion procedure through a 100-nm pores polycarbonate filter, as previously described [38]. Phospholipid recovery after extrusion was determined by phosphorous assay using the method of Stewart [39].…”

Section: Nl Preparationmentioning

confidence: 99%

“…All NL preparations were characterized in terms of size, ζ-potential, polydispersity index and stability by DLS, as described [38].…”

Section: Characterization Of Nlmentioning

confidence: 99%

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Functionalization with TAT-Peptide Enhances Blood-Brain Barrier Crossing In vitro of Nanoliposomes Carrying a Curcumin-Derivative to Bind Amyloid-Β Peptide

Sancini

Gregori

Salvati

et al. 2013

J Nanomedic Nanotechnol

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# These authors contributed equally to this work preventing high-molecular weight molecules from passing through [25]. Indeed, the design and engineering of NP with high specificity for brain capillary endothelial cells have been proposed as promising strategy for AD diagnosis and treatment [26][27][28][29].The aim of the present investigation was to design NP able to bind Aβ peptide and to cross the BBB. To reach this goal we developed nanoliposomes (NL) double-functionalized with a curcuminderivative and with a modified HIV Transactivating Transcriptional Activator (TAT) peptide. Preparation of NL covalently decorated with curcumin-derivative was previously carried out, starting from a curcumin alkyne-derivative showing a very high affinity for Aβ peptide [30], suitable for NL decoration by click chemistry and with improved features of stability with respect to curcumin itself [31]. On the other side, TAT-peptide could enhance NP BBB crossing [32,33] based on the evidence that its coupling to NP may facilitate their efficient translocation through the cell membrane, bypassing the endocytic pathway [34][35][36]. TAT-peptide was covalently attached to NL surface via a thiol-maleimide reaction. The ability of NL to bind Aβ after AbstractProduction of abnormally high amounts of amyloid-β peptide in the brain plays a central role in the onset and development of Alzheimer's disease, a neurodegenerative disorder affecting millions of individuals worldwide. Nanoparticles have been proposed as promising tools to treat the disease by delivering drugs and contrast agents to the brain. Here, nanoliposomes decorated with a curcumin-derivative, displaying high affinity for amyloid-β, were functionalized with a modified cell-penetrating TAT-peptide, with the aim of conferring on such nanoliposomes the ability to cross the blood-brain barrier. Functionalization with TAT-peptide did not modify the ability of curcumindecorated nanoliposomes to bind amyloid-β fibrils, as assessed by surface plasmon resonance. Confocal microscopy, mass spectrometry and radioactivity experiments with [3 H]-sphingomyelin showed about 3-fold increase in the uptake of nanoliposomes by human brain capillary endothelial cells (hCMEC/D3) after the functionalization with TATpeptide, with no alterations in cell viability. Moreover, TAT functionalization increased the permeability of curcuminnanoliposomes across a blood-brain barrier model made with the same cells. The similar permeabilities of curcuminderivative and [3 H]-sphingomyelin suggested that nanoliposomes were transported intact. Considering these results, nanoliposomes functionalized with the curcumin-derivative and TAT-peptide represent a promising tool for targeting amyloid-β directly in the brain parenchyma.

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“…17 Moreover, to dock Aβ for improved neuroprotection, CRM and NGF may be loaded in LIP-containing cardiolipin (CL). 18 This is due to a strong affinity of CL to Aβ. 19 Thus, inclusion of CL may enhance the ability of LIP to target Aβ around apoptotic neurons.…”

Section: Introductionmentioning

confidence: 99%

Wheat germ agglutinin-conjugated liposomes incorporated with cardiolipin to improve neuronal survival in Alzheimer’s disease treatment

Kuo¹,

Lin²,

Li³

et al. 2017

IJN

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Curcumin (CRM) and nerve growth factor (NGF) were entrapped in liposomes (LIP) with surface wheat germ agglutinin (WGA) to downregulate the phosphorylation of kinases in Alzheimer’s disease (AD) therapy. Cardiolipin (CL)-conjugated LIP carrying CRM (CRM-CL/LIP) and also carrying NGF (NGF-CL/LIP) were used with AD models of SK-N-MC cells and Wistar rats after an insult with β-amyloid peptide (Aβ). We found that CRM-CL/LIP inhibited the expression of phosphorylated p38 (p-p38), phosphorylated c-Jun N-terminal kinase (p-JNK), and p-tau protein at serine 202 and prevented neurodegeneration of SK-N-MC cells. In addition, NGF-CL/LIP could enhance the quantities of p-neurotrophic tyrosine kinase receptor type 1 and p-extracellular signal-regulated kinase 5 for neuronal rescue. Moreover, WGA-grafted CRM-CL/LIP and WGA-grafted NGF-CL/LIP significantly improved the permeation of CRM and NGF across the blood–brain barrier, reduced Aβ plaque deposition and the malondialdehyde level, and increased the percentage of normal neurons and cholinergic activity in the hippocampus of AD rats. Based on the marker expressions and in vivo evidence, current LIP carriers can be promising drug delivery systems to protect nervous tissue against Aβ-induced apoptosis in the brain during the clinical management of AD.

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Lipid-based nanoparticles with high binding affinity for amyloid-β1–42 peptide

Cited by 187 publications

References 55 publications

The effect of nanoparticle uptake on cellular behavior: disrupting or enabling functions?

The effect of nanoparticle uptake on cellular behavior: disrupting or enabling functions?

Functionalization with TAT-Peptide Enhances Blood-Brain Barrier Crossing In vitro of Nanoliposomes Carrying a Curcumin-Derivative to Bind Amyloid-Β Peptide

Wheat germ agglutinin-conjugated liposomes incorporated with cardiolipin to improve neuronal survival in Alzheimer’s disease treatment

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Lipid-based nanoparticles with high binding affinity for amyloid-β1–42 peptide

Cited by 187 publications

References 55 publications

The effect of nanoparticle uptake on cellular behavior: disrupting or enabling functions?

The effect of nanoparticle uptake on cellular behavior: disrupting or enabling functions?

Functionalization with TAT-Peptide Enhances Blood-Brain Barrier Crossing In vitro of Nanoliposomes Carrying a Curcumin-Derivative to Bind Amyloid-Β Peptide

Wheat germ agglutinin-conjugated liposomes incorporated with cardiolipin to improve neuronal survival in Alzheimer&rsquo;s disease treatment

Contact Info

Product

Resources

About

Wheat germ agglutinin-conjugated liposomes incorporated with cardiolipin to improve neuronal survival in Alzheimer’s disease treatment