Lipid and γ‐linolenic acid accumulation in strains of zygomycetes growing on glucose

Kavadia, A.; Komaitis, Michael; Chevalot, Isabelle; Blanchard, Fabrice; Marc, Ivan; Aggelis, George

doi:10.1007/s11746-001-0266-3

Cited by 106 publications

(101 citation statements)

References 17 publications

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“…In a similar manner, when Mucor circinelloides CBS 172-27 was cultured on sunflower oil, during storage lipid breakdown unsaturated fatty acids were preferentially oxidized [6]. In contrast, other Zygomycetes (genera Rhizopus, Cunninghamella and Zygorhynchus) preferentially oxidized C18:0, while γ-linolenic acid ( ∆6,9,12 C18:3) content increased during storage lipid breakdown [20,21]. Likewise, oleaginous bacteria of the genus Rhodococcus, which had accumulated both TAGs and polyhydroxyalkanoates (PHAs), preferentially mobilized TAGs whereas PHAs breakdown was delayed [22].…”

Section: Discussionmentioning

confidence: 88%

Selective uptake of fatty acids by the yeast Yarrowia lipolytica

Papanikolaou

Aggelis

2003

Euro J Lipid Sci & Tech

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Yarrowia lipolytica ACA-DC 50109, maintained on potato dextrose agar (PDA, Fluka, Hannover, Germany) at T = 4 °C, was used. The salt composition of the culture medium was (g/l): KH 2 PO 4 7, Na 2 HPO 4 2. Selective uptake of fatty acids by the yeast Yarrowia lipolyticaYarrowia lipolytica, when cultivated on mixtures of free fatty acid substrates was found to remove C12:0, C14:0, ∆9 C18:1, ∆9,12 C18:2 and ∆9,12,15 C18:3 at significantly higher rates than C16:0 and C18:0, regardless of fatty acid composition of the initial substrate. C12:0, C14:0 and ∆9,12,15 C18:3 were specifically and completely removed from the substrate, while ∆9 C18:1 and ∆9,12 C18:2 concentrations decreased by 55-80 wt-% in comparison with initial concentrations. In contrast, concentration of C18:0 increased 2.1-3.5 fold in the substrate. Although C18:0 was removed slowly from the substrate, this fatty acid was selectively accumulated in the storage lipid. Inversely, only low quantities of ∆9 C18:1 and ∆9,12 C18:2 and traces of C12:0, C14:0 and ∆9,12,15 C18:3 were accumulated in the storage lipid. During storage lipid breakdown, cellular C16:0 and ∆9 C18:1 were taken up more rapidly than C18:0. We concluded that the capability of Yarrowia lipolytica to selectively remove several fatty acids from the substrate and accumulate others could be used in modification of the composition of selected mixtures of fatty acids and probably of common fats, to produce "new" fats with a predetermined composition.

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Section: Discussionmentioning

confidence: 88%

Selective uptake of fatty acids by the yeast Yarrowia lipolytica

Papanikolaou

Aggelis

2003

Euro J Lipid Sci & Tech

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“…As for GLA, it was found in remarkable quantities in all lipid fractions, especially in P fraction, reaching up to 21.4%, 19.9%, and 19.8% in Z. moelleri, C. echinulata, and Mucor sp., respectively. These fungi are known as GLA producers [3,6,7,17,47]. P is the site of PUFA production [16] and therefore its fatty acid composition provides a description concerning desaturase activity in the mycelium.…”

Section: Fatty Acid Composition Of Lipid Fractionsmentioning

confidence: 99%

“…Oleaginous Zygomycetes have the ability to accumulate large amounts of lipids (namely single cell oils-SCO) rich in polyunsaturated fatty acids (PUFA) [1][2][3][4][5][6][7][8][9][10], which are of significant pharmaceutical and nutritional value. Among PUFA, γ-linolenic acid (GLA) is of particular interest as it is known for its selective killing of tumor cells without harming normal cells [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Lipids Containing Polyunsaturated Fatty Acids Synthesized by Zygomycetes Grown on Glycerol

Bellou

Moustogianni

Makri

et al. 2011

Appl Biochem Biotechnol

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Several strains of Zygomycetes cultivated on glycerol produced mycelia rich in lipids containing higher amounts of neutral lipids (NL) than glycolipids plus sphingolipids and phospholipids (P), while biosynthesis of P in Mortierella ramanniana, Mucor sp., and Cunninghamella echinulata occurred though NL accumulation process was in progress. Polyunsaturated fatty acids (PUFA) concentration gradually decreased in all lipid fractions of M. ramanniana during growth. In contrast, in C. echinulata concentration of both linoleic and γ-linolenic acids increased with time, especially in P. Taking for granted that the main function of PUFA is associated to their participation in mycelial membranes, we could suppose that biosynthesis of these fatty acids is associated to mycelial growth. However, this is accurate only for some Zygomycetes, e.g., M. ramanniana. On the contrary, PUFA biosynthesis in C. echinulata persists after growth cessation, suggesting that in this species biosynthetic ability is not a strictly growth-associated process. Phosphatidyl-inositol and phosphatidyl-choline were the major P classes in C. echinulata and M. ramanniana, respectively. In M. ramanniana, a decrease of PUFA concentration was noticed even when mycelia were incubated in low temperature (conditions that normally favor PUFA biosynthesis), indicating that PUFA biosynthesis in this fungus is associated to primary metabolism.

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“…Several studies on Mortierella and other fungi have shown that media variables such as different carbon and nitrogen sources, pH of the growth medium and the composition of trace elements affect the growth and lipid accumulation that occur within various species [3,4,5,6] . Of particular importance is the effect of metal ions on lipid and GLA production.…”

Section: Introductionmentioning

confidence: 99%

Effects of Metal Ion Concentrations on Lipid and Gamma Linolenic Acid Production by Cunninghamella sp. 2A1

Muhid¹,

Nawi²,

Kader³

et al. 2008

OnLine J. of Biological Sciences

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O on lipid and GLA production by Cunninghamella sp. 2A1 were studied in submerged culture fermentation. Cultivation was performed in 250 ml conical flask (100ml working volume), at 30°C and 250 rpm agitation for 120h. Mg 2+ , Fe 2+ and Zn 2+ have significant effect on lipid accumulation as inclusion of each ions resulted in a 64, 43 and 33% increase in lipid content compared to cultures which was cultivated with the omission of each ions, respectively. All three metal ions also affected GLA production by Cunninghamella sp. 2A1 with Zn 2+ having the most profound effect with an increase of up to 74% of GLA yield (g GLA/g lipidless biomass) achieved. As omission of any single metal ions did not affect biomass concentration achieved, we concluded that metal ions components in production media may have affected lipid and GLA production by affecting pathways involved in the biosynthesis of lipid and GLA. This study reports the critical concentration of each metal ion for optimal lipid and GLA production by Cunninghamella sp. 2A1.

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Lipid and γ‐linolenic acid accumulation in strains of zygomycetes growing on glucose

Cited by 106 publications

References 17 publications

Selective uptake of fatty acids by the yeast Yarrowia lipolytica

Selective uptake of fatty acids by the yeast Yarrowia lipolytica

Lipids Containing Polyunsaturated Fatty Acids Synthesized by Zygomycetes Grown on Glycerol

Effects of Metal Ion Concentrations on Lipid and Gamma Linolenic Acid Production by Cunninghamella sp. 2A1

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