Lipid and oxylipin profiles during aging and sprout development in potato tubers (Solanum tuberosum L.)

Abstract

Potato tubers (Solanum tuberosum L. cv Bintje) were stored at 20 jC for 210 days without desprouting to study the lipoxygenase pathway during aging. After 15 days of storage, potato tubers sprouted, while after 45 -60 days, apical dominance was lost and multiple sprouts developed. Analysis of the fatty acid hydroperoxides (HPOs) revealed that 9-S-hydroperoxide of linoleic acid (9-HPOD) was the main oxylipin formed. Between 45 and 60 days of storage, increases in the levels of 9-HPOD and colneleic acid were observed. Analysis of phospholipids and galactolipids by electrospray ionisation tandem mass spectrometry (ESI-MS/MS) showed that a decrease in the levels of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), digalactosyldiacylglycerol (DGDG), and monogalactosyldiacylglycerol (MGDG) occurred between 0 and 45 days of aging. The decrease in the amount of linoleic acid in complex lipids correlates well with the amount of 9-HPOD and colneleic acid produced. D

Release of Apical Dominance in Potato Tuber Is Accompanied by Programmed Cell Death in the Apical Bud Meristem

“…Nevertheless, both treatments resulted in reduced dominance of the tuber apical bud, suggesting a relationship with their ability to induce PCD in the apical and lateral bud meristems. AD is known to be released in stored potato tubers during aging (Krijthe, 1962;Kumar and Knowles, 1993b), and tuber aging has been correlated with the loss of membrane integrity due to peroxidative damage, the activities of scavenging enzymes, and increased lipid oxidation (Kumar and Knowles, 1993a;Fauconnier et al, 2003;Delaplace et al, 2008). Although those studies measured oxidative changes in the tuber parenchyma, such changes might be associated with developmental or environmentally induced PCD, as sprouting is part of tuber aging in storage.…”

Molecular Cloning, Functional Expression, and Tissue Distribution of a Potato Sprout Allene Oxide Synthase Involved in a 9-Lipoxygenase Pathway

“…[12][13][14][15] The major metabolism of 9-HPOD and 9-HPOT in potato plants is the formation of divinyl ether compounds, catalyzed by DES, 14,16) and the formation of epoxy alcohol compounds by EAS. 17,18) The potato DES and EAS pathways have been characterized in detail, but information about AOS, involved in the 9-LOX pathway, is still limited.…”

“…[9][10][11] Since potato (Solanum tuberosum) plants show strong 9-LOX activity, they are often used to study the physiological function of 9-LOXs and related oxylipins. [12][13][14][15] The characteristic metabolism of 9-HPOD and 9-HPOT in potato plants is the formation of divinyl ether 14,16) and epoxy alcohol compounds. 17,18) The epoxy alcohol compounds are readily converted to trihydroxy fatty acids.…”

Plant lipidomics: Discerning biological function by profiling plant complex lipids using mass spectrometry

“…Various studies have been undertaken to understand the role of lipid peroxidation in membrane damage and, in general, in the aging process (39)(40)(41)(42). Focusing particularly on the sprouting phase (42), it has been demonstrated that the period during which apical dominance is lost, resulting in the development of multiple sprouts, is correlated with a clear increase in particular oxygenated fatty acids, or "oxylipins", specifically the 9-hydroperoxide of linoleic acid and colneleic acid (42). Analysis of phospholipids and galactolipids by electrospray ionization tandem mass spectrometry showed a decrease in the level of GPCho, GPEtn, GPIns, GalGalDG, and GalDG during this particular period.…”