Lipid accumulation in cells derived from porcine aorta and grown under anaerobic conditions

Abstract

Fibroblast-like cells, derived from porcine aorta, were cultured under aerobic and anaerobic conditions. Light and electron microscopic examinations, lipid composition measurements, and incorporation of radioactive precursors into lipids of these cells were performed. Anaerobically grown cells accumulated oil red O stainable droplets and within 6 hr the triacylglycerol content increased to 4 times the level determined in cells grown under aerobic conditions. This ratio remained constant throughout an additional 12 hr of growth. The fatty acid composition of the triacylglycerols which accumulated under anaerobic conditions differed from the composition of fatty acids in the triacylglycerols present in the growth medium. The cellular unesterified fatty acids of the anaerobically grown cells differed only slightly in composition from the fatty acids in the growth medium, while the unesterfied fatty acids of aerobically grown cells differed to a greater extent from those of the growth medium.

Mesenchymal Stem Cells Respond to Hypoxia by Increasing Diacylglycerols

“…Our lipidomic analysis revealed a strong up-regulation of many lipids in MSC exposed for 48 h to hypoxia, similarly to other cell types [Briggs and Glenn, 1976;Gordon et al, 1977], As previously described in fibroblasts [Gordon et al, 1977], TGs and FAs were significantly increased under hypoxia, a phenomenon that could be explained by the inability of hypoxic cells to support the oxygendependent fatty acid oxidation [Bhatnagar, 2003].…”

Calcium-dependent action of osmolality on adenosine 3',5'-monophosphate accumulation in rat renal inner medulla: evidence for a relationship to calcium-responsive arachidonate release and prostaglandin synthesis.

“…The CHC13 extract was evaporated to dryness under N2 and redissolved in 1 ml of CHC13. Prostaglandins of the A, E, and F series were isolated by silicic acid chromatography (14) after preliminary separation of prostaglandins from neutral lipids and phospholipids (15,16 (18).…”

“…Lipids were separated into major classes by silicic acid chromatography (15,16). Extracts were dissolved in 1 ml CHC13 and applied to silicic acid columns prepared in CHC13.…”