Lipid accumulation during nitrogen and sulfur starvation in Chlamydomonas reinhardtii overexpressing a transcription factor

Salas-Montantes, Carlos Joel; González‐Ortega, Omar; Ochoa-Alfaro, Ana Erika; Camarena-Rangel, Rodrigo; Paz-Maldonado, Luz María Teresita; Rosales‐Mendoza, Sergio; Rocha‐Uribe, Alejandro; Soria-Guerra, Ruth Elena

doi:10.1007/s10811-018-1393-6

Cited by 42 publications

(18 citation statements)

References 67 publications

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“…In transgenic strain, the expression of the enoyl-ACP-reductase (ENR1) and the sulfolipid synthase (SQD2) genes was greatly up-regulated, which is a key gene related to fatty acid synthase (FAS) and the glycerolipid biosynthesis, respectively [ 88 ]. Furthermore, Salas-Montantes et al [ 89 ] investigated the feasibility for improving lipid accumulation of C. reinhardtii under nutrient deficiency stress by overexpression of a Dof-type TF (Dof11), and results showed that the lipid content and the proportion of specific fatty acids were all increased. Similarly, a combination of the overexpression of GmDof4 and mixotrophic culture condition was investigated in microalgae Chlorella ellipsoidea , and results showed that the lipid content was increased from 46.4 to 52.9% with no impact over the growth rate [ 90 ].…”

Section: Countering the Negative Effects Of Stress Via Transcription mentioning

confidence: 99%

Microalgae for the production of lipid and carotenoids: a review with focus on stress regulation and adaptation

Sun

Ren

Zhao

et al. 2018

Biotechnol Biofuels

321

129

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Microalgae have drawn great attention as promising sustainable source of lipids and carotenoids. Their lipid and carotenoids accumulation machinery can be trigged by the stress conditions such as nutrient limitation or exposure to the damaging physical factors. However, stressful conditions often adversely affect microalgal growth and cause oxidative damage to the cells, which can eventually reduce the yield of the desired products. To overcome these limitations, two-stage cultivation strategies and supplementation of growth-promoting agents have traditionally been utilized, but developing new highly adapted strains is theoretically the simplest strategy. In addition to genetic engineering, adaptive laboratory evolution (ALE) is frequently used to develop beneficial phenotypes in industrial microorganisms during long-term selection under specific stress conditions. In recent years, many studies have gradually introduced ALE as a powerful tool to improve the biological properties of microalgae, especially for improving the production of lipid and carotenoids. In this review, strategies for the manipulation of stress in microalgal lipids and carotenoids production are summarized and discussed. Furthermore, this review summarizes the overall state of ALE technology, including available selection pressures, methods, and their applications in microalgae for the improved production of lipids and carotenoids.

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Section: Countering the Negative Effects Of Stress Via Transcription mentioning

confidence: 99%

Microalgae for the production of lipid and carotenoids: a review with focus on stress regulation and adaptation

Sun

Ren

Zhao

et al. 2018

Biotechnol Biofuels

321

129

View full text Add to dashboard Cite

show abstract

“…2011, Salas‐Montantes et al. 2018, Meng et al. 2020), or as a new tool to create offspring with new traits (Nour‐Eldin et al.…”

Section: Discussionmentioning

confidence: 99%

Exogenous Abscisic Acid Confers Salinity Tolerance in Chlamydomonas reinhardtii During Its Life Cycle

Abu‐Ghosh

Iluz

Dubinsky

et al. 2021

Journal of Phycology

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The plant hormone abscisic acid (ABA) coordinates responses to environmental signals with developmental changes and is important for stress resilience and crop yield. However, fundamental questions remain about how this phytohormone affects microalgal growth and stress regulation throughout the different stages of their life cycle. In this study, the effects of ABA on the physiology of the freshwater microalga Chlamydomonas reinhardtii at its different life cycle stages were investigated. Exogenously added ABA enhanced the growth and photosynthesis of C. reinhardtii during the vegetative stage. The hormone also increased the tolerance of this alga to high‐salinity stress during gamete formation under nutrient depletion, as well as it extended their survival. We show that the level of reactive oxygen species (ROS) generated in the ABA‐treated cells was significantly less than that in the untreated cells under inhibiting NaCl concentrations. Cell size examination showed that ABA prevents cells from forming palmella when exposed to high salinity. All together, these results suggest that ABA can support the vitality and survival of C. reinhardtii under high salt conditions.

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“…The second program is that the vegetative cells underdo gametogenesis to produce Mt+ and Mt-cells for mating. While N starvation has been extensively studied in Chlamydomonas for producing alternative sources of energy by accumulation more TAG in the cells under N starvation (Montantes et al, 2018;Salas-Siaut et al, 2011;Yang et al, 2020), or as a new tool to create offspring with new traits (Kramer and Lucker, 2020), however, N-starved Chlamydomonas cells can also exhibit various biological pathways for managing photosynthesis to efficiently utilize the absorbed light energy (Saroussi et al, 2017). While underlying these pathways is still in its early stage, it could provide an important direction for developing a more comprehensive understanding of photosynthetic energetics and its control.…”

Section: Discussionmentioning

confidence: 99%

Abscisic acid confers salinity tolerance in Chlamydomonas reinhardtii at different stages of its life cycle

Abu‐Ghosh¹,

Dubinsky²,

Iluz³

2020

Preprint

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The effect of abscisic acid (ABA) to increase the osmotolerance of the freshwater microalga Chlamydomonas reinhardtii at its different stages of life cycle was investigated. Exogenously added ABA enhanced the growth and photosynthesis of C. reinhardtii during the vegetative stage. The hormone also increased the tolerance of this alga to oxidative stress during gamete formation under nutrient depletion and high salinity, as it supported their survival for a longer period. We show that the level of reactive oxygen species (ROS) generated in the ABA-treated cells was significantly less than that in the untreated cells under inhibiting NaCl concentrations. Cell size examination showed that ABA prevents cells from forming palmella when exposed to high salinity. All together, these results suggest that ABA increases the tolerance of C. reinhardtii to salt stress conditions.

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Lipid accumulation during nitrogen and sulfur starvation in Chlamydomonas reinhardtii overexpressing a transcription factor

Cited by 42 publications

References 67 publications

Microalgae for the production of lipid and carotenoids: a review with focus on stress regulation and adaptation

Microalgae for the production of lipid and carotenoids: a review with focus on stress regulation and adaptation

Exogenous Abscisic Acid Confers Salinity Tolerance in Chlamydomonas reinhardtii During Its Life Cycle

Abscisic acid confers salinity tolerance in Chlamydomonas reinhardtii at different stages of its life cycle

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