Lipid accumulation and growth characteristics of Chlorella zofingiensis under different nitrate and phosphate concentrations

Feng, Pingzhong; Deng, Zhongyang; Lü, Fan; Hu, Zhengyu

doi:10.1016/j.jbiosc.2012.05.007

Cited by 141 publications

(61 citation statements)

References 25 publications

(28 reference statements)

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“…P limitation also appears to stimulate lipid accumulation, as found by Rhee (1978); i.e., the lipid content was higher when P was unavailable. However, most studies have found that N deficiency produces a higher percentage of lipid than P deficiency (Illman et al 2000, Mandal and Mallick 2009, Feng et al 2012. With respect to temperature, one study determined that raising the temperature from 30°C to 42°C increased the lipid content of the bluegreen alga Spirulina maxima (Paoletti et al 1980).…”

Section: Introductionmentioning

confidence: 99%

Influence of temperature and nutrient content on lipid production in freshwater microalgae cultures

Bohnenberger

Crossetti

2014

An. Acad. Bras. Ciênc.

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The production of biomass by microalgae is considered a clean alternative compared to other plant crops that require large areas for cultivation and that generate environmental impacts. This study evaluated the influence of temperature and nutrients on lipid contents of cultured species of freshwater microalgae, with a view toward using these lipids for biodiesel production. Two strains of Monoraphidium contortum, a culture containing Chlorella vulgaris and Desmodesmus quadricauda and another strain of Microcystis aeruginosa were maintained in the laboratory for six days, in five culture media: modified ASM-1 (control, with high concentrations of phosphate and nitrate; phosphorus-deficient; non-limiting phosphate; nitrogendeficient; and non-limiting nitrate). The cultures were then exposed to temperatures of 13°C, 25°C (control) and 37°C for eight days (n=3). Lipids were extracted by the cold-solvent (methanol and chloroform) method. On average, the highest total lipid yields were observed when the strains were maintained at 13°C and in the non-limiting nitrate medium. The lipid percentage varied depending on the concentration of algal biomass. This study showed that manipulation of controlling factors can increase the lipid concentration, optimizing the total production in order to use this raw material for biodiesel.

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Section: Introductionmentioning

confidence: 99%

Influence of temperature and nutrient content on lipid production in freshwater microalgae cultures

Bohnenberger

Crossetti

2014

An. Acad. Bras. Ciênc.

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“…Phosphate limitation is known to decrease the Chlorella zofingiensis biomass (Feng et al, 2012), and also due to the reduction in nitrate uptake by the phosphate-limiting conditions, which affects cell proliferation (Xin et al, 2010). However, excess phosphate can cause phosphate toxicity in cells (Feng et al 2012) reducing cell density and biomass (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…However, excess phosphate can cause phosphate toxicity in cells (Feng et al 2012) reducing cell density and biomass (Fig. 2B).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, Chlorella is also known to be sensitive to the changes in the abiotic and biotic factors in culture conditions. Thus, suitability of microalgae species, adequate cultural methods, ability to grow rapidly, and accumulation of large amounts of lipids are important factors in increasing the economic feasibility of lipid production (Rawat et al, 2013;Feng et al, 2012) and reducing the financial burden on algal companies (Wang et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…The manipulation of culture medium composition is one of the strategies for achieving higher production of the desired target compounds. Several factors such as nutrient stress (Cha et al, 2011;Feng et al, 2012;George et al, 2014;Prochazkova et al, 2014;Kamalanathan et al, 2015;Shen et al, 2016), light (Abu-Gosh et al, 2016;Cheirsilp and Torpee, 2012), osmotic stress, radiation, pH, temperature, heavy metals, and other chemicals (Sharma et al, 2012) have been shown to increase the lipid content of microalgae. Phosphorous is an important macronutrient that contributes to various metabolic processes such as signaling pathways, energy generation, and photosynthesis.…”

Section: Introductionmentioning

confidence: 99%

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Phosphate-induced changes in fatty acid biosynthesis in Chlorella sp. KS-MA2 strain

Aziz¹,

Osman²,

San³

et al. 2016

bta

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Phosphate is involved in fatty acid biosynthesis in microalgae cells. The influence of phosphate concentrations on polyunsaturated fatty acids' profile, elongation, and desaturation of fatty acids chains during their biosynthesis still remains unclear. Protein ketoacyl-ACP synthase-I (KAS-1 ) is required for the addition of malonyl-CoA for the elongation of butyryl-ACP from a 4-to 14-carbon chain. The omega-6 (ω-6 FAD ) and omega-3 desaturases (ω-3 FAD ) are involved in the insertion of double bonds into the pre-formed fatty acid chains. This study reports the effect of phosphate concentrations on the growth, fatty acid content, and level of KAS-1, ω-6 FAD, and ω-3 FAD transcripts in Chlorella KS-MA2 strain at the stationary growth phase. Phosphate was tested at concentrations 9, 18, 36, and 72 μM and the fatty acid content was analyzed using gas chromatography. Gene expression levels were measured using quantitative real-time-PCR. Results showed that cell growth, amount of saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), and polyunsaturated fatty acids (PUFAs) did not significantly (P > 0.05) differ among tested treatments. The highest concentration of α-linolenic acid and the expression level of ω-3 FAD were recorded with 72 μM of phosphate in culture medium. The KAS-1 and ω-6 FAD transcripts levels decreased when the concentration of phosphate was below 36 μM. Phosphate in F/2 medium was sufficient to enhance the biosynthesis of major fatty acids in Chlorella sp.

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Sustainability, Commercialization, and Future Prospects of Biodiesel Production

Vairaprakash

Arumugam

2022

Biodiesel Production

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Lipid accumulation and growth characteristics of Chlorella zofingiensis under different nitrate and phosphate concentrations

Cited by 141 publications

References 25 publications

Influence of temperature and nutrient content on lipid production in freshwater microalgae cultures

Influence of temperature and nutrient content on lipid production in freshwater microalgae cultures

Phosphate-induced changes in fatty acid biosynthesis in Chlorella sp. KS-MA2 strain

Sustainability, Commercialization, and Future Prospects of Biodiesel Production

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