Lipid A, The Endotoxic Principle Of Bacterial Lipopolysaccharides: Chemical Structure And Biological Activity

Rietschel, Ernst; Brade, Helmut; Brade, Lore; Kawahara, Kazuyoshi; Lüderitz, Th.; Schade, Ulrich; Tacken, Angelika; Zähringer, Ulrich

doi:10.1515/9783110874297-042

Cited by 33 publications

(35 citation statements)

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“…Thus, LPSs of the P. fluorescens strains studied exhibited considerable similarity in the composition and structural characteristics of the lipid moiety of the macromolecule, which determines the endotoxic activity of the LPS molecule (according to current understanding [4]). Thus, the LPS preparations studied were somewhat different with respect to the composition of the core oligosaccharides and completely different with respect to the structure of the O -chain, as judged by the absence of serological relationships.…”

Section: Resultsmentioning

confidence: 99%

“…In this work, we performed a comparative study of biological properties of LPSs from P. fluorescens strains and LPS from E. coli strains , which served as a positive control. A characteristic feature of this lipid A is that it consists of a disaccharide comprised of glucosamine residues substituted with two phosphate groups and six residues of fatty acids with 14 and 12 carbon atoms (predominantly the 3-OH-C 14:0 fatty acid) [4]. Such a difference may be due to physiological and biochemical properties of these microorganisms, whose LPSs differ from the LPS from E. coli with respect to the structure of lipid A-the endotoxic center of the LPS macromolecule [13].…”

Section: Resultsmentioning

confidence: 99%

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Structural Characteristics and Biological Properties of Pseudomonas fluorescens Lipopolysaccharides

Sn¹,

2005

Appl Biochem Microbiol

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The structure and biological properties of lipopolysaccharides (LPSs) from strains IMB 4125 (=ATCC 13525) and IMB 7769 of the bacterium Pseudomonas fluorescens (biovar I) were studied in vitro. LPSs were similar in the composition of lipid A and the core lipid but differed in the structure of O -specific polysaccharide chains, which was corroborated by the absence of serological relationships between them. The toxicity (LD 50 ) of LPSs of P. fluorescens with respect to D -glucosamine-sensitized mice was 40-50 times lower than the toxicity of the classic endotoxins, LPSs of E. coli. The LPSs studied stimulated the production of tumor necrosis factor (TNF) and nitric oxide (NO) by mouse peritoneal macrophages. The rates of TNF and NO synthesis induced by the LPSs of interest were eight to nine and three to five times lower, respectively, than the corresponding parameters of the control LPSs of E. coli 055:B5 and 026:B6. Additionally, LPS preparations of the P. fluorescens strains induced TNF synthesis by monocytes of human whole-blood preparations. Certain differences in biological properties of these strains have been revealed, which could be due to the characteristic features of LPS structure and composition in different cultures.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Structural Characteristics and Biological Properties of Pseudomonas fluorescens Lipopolysaccharides

Sn¹,

2005

Appl Biochem Microbiol

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“…LPS, which is found in the outer membrane of gram negative bacteria, is the most widely studied of the TLR4 ligands, and virtually all clinical trials involving TLR4 adjuvants examine derivatives of LPS. LPS is a complex molecule, and it is the lipid A portion composed of polyacylated diglucosamine lipids that mediates interactions with TLR4 [84;85]. Although the immunostimulatory capacity of LPS has been known for decades, the intact molecule is highly toxic, preventing it's use as a vaccine adjuvant [86].…”

Section: Tlr4mentioning

confidence: 99%

TLR-based immune adjuvants

Steinhagen

Kinjo

Bode

et al. 2011

Vaccine

432

372

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“…(b) Chemical structures of the lipid A component of the LPS samples used in this study [41–43]. (c) 4–20% SDS-PAGE separation of the LPS samples used in this study.…”

Section: Figurementioning

confidence: 99%

Molecular Characterization of Lipopolysaccharide Binding to Humanα-1-Acid Glycoprotein

et al. 2012

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The ability of AGP to bind circulating lipopolysaccharide (LPS) in plasma is believed to help reduce the proinflammatory effect of bacterial lipid A molecules. Here, for the first time we have characterized human AGP binding characteristics of the LPS from a number of pathogenic Gram-negative bacteria: Escherichia coli, Salmonella typhimurium, Klebsiella pneumonia, Pseudomonas aeruginosa, and Serratia marcescens. The binding affinity and structure activity relationships (SAR) of the AGP-LPS interactions were characterized by surface plasma resonance (SPR). In order to dissect the contribution of the lipid A, core oligosaccharide and O-antigen polysaccharide components of LPS, the AGP binding affinity of LPS from smooth strains, were compared to lipid A, Kdo2-lipid A, Ra, Rd, and Re rough LPS mutants. The SAR analysis enabled by the binding data suggested that, in addition to the important role played by the lipid A and core components of LPS, it is predominately the unique species- and strain-specific carbohydrate structure of the O-antigen polysaccharide that largely determines the binding affinity for AGP. Together, these data are consistent with the role of AGP in the binding and transport of LPS in plasma during acute-phase inflammatory responses to invading Gram-negative bacteria.

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Lipid A, The Endotoxic Principle Of Bacterial Lipopolysaccharides: Chemical Structure And Biological Activity

Cited by 33 publications

References 0 publications

Structural Characteristics and Biological Properties of Pseudomonas fluorescens Lipopolysaccharides

Structural Characteristics and Biological Properties of Pseudomonas fluorescens Lipopolysaccharides

TLR-based immune adjuvants

Molecular Characterization of Lipopolysaccharide Binding to Humanα-1-Acid Glycoprotein

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