Lipemia and bilirubin influences for twenty-four biochemical parameters measurement

Abstract

L'étude de l'influence de la lipémie et de l'ictère a été réalisée expérimentalement pour 24 paramètres de biochimie sur l'automate Cobas 6000 Roche. Des surcharges en Intralipid ® ou en ditaurate de bilirubine ont été effectuées sur différents pools plasmatiques. La limite de 10 % a été choisie pour définir une interférence sur la mesure. Les paramètres étudiés ont été classés en différentes catégories selon que leur mesure soit impactée ou non. La connaissance de ces données permet au biologiste d'adapter ses modalités de compte rendu dans le cas d'échantillons lactescents ou/et ictériques.Abstract. The study of the influence of the lipemia and icterus was performed experimentally for twenty-four biochemistry parameters on the Roche Cobas 6000 CE analyzer. Overloads in Intralipid ® or ditaurate of bilirubin were performed on several plasma pools. The limit of 10% was chosen to define interference on the measurement. The parameters studied were classified into different categories depending on their measurement is affected or not. Knowledge of these data allows the biologist to adapt its reporting procedures in the case of lactescent and/or icteric samples.

Comparison of lipemia interference created with native lipemic material and intravenous lipid emulsion in emergency laboratory tests

“…Statistically and clinically significant negative interference was detected for alanine aminotransferase (ALT) and aspartate aminotransferase (AST), consistent with previous studies ( 14 , 31 , 32 ). When lipemia index values were 159 and 174 for NULM and IVLE, respectively, the negative interference amount exceeded the 10% limit, conforming to the manufacturer’s declaration (Supplementary table 2).…”

“…Interference evaluations were made for biochemistry parameters using IVLE on the Roche Cobas 6000 analyzer and these data were published before ( 31 , 32 ). Our results were evaluated by comparison with these studies and, where available, with test-based studies.…”

Icteric human samples: Icterus index and method of estimating an interference‐free value for 16 biochemical analyses

“…As these two parameters are available, it is possible to estimate the true analyte level. However, Ali et al 18 show that there are two levels of interference for PROT, tested at 30 and 74 g/L, which are outside our studied range. For TG, due to the nature of the relationship, with I ict and % of interference, two equations are needed.…”

Interferences in the measurement of circulating phosphate: a literature review

“…Spurious hyperphosphatemia was documented in 6 patients [21] with severe hyperbilirubinemia (>330 µmol/L). This tendency was confirmed in 4 reports examining the interference from bilirubin on the determination of this ion [22][23][24][25].…”