Lipases in racemic resolutions

Muralidhar, R. V.; Marchant, Roger; Nigam, Poonam Singh Nee

doi:10.1002/1097-4660(200101)76:1<3::aid-jctb336>3.0.co;2-8

Cited by 45 publications

(22 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to their role in synthetic organic chemistry, these also find extensive applications in chemical, pharmaceutical, food and leather industries (Gulati et al, 2005;Gunstone, 1999). Promising fields for the application of lipases also include the biodegradation of Plastics (Gombert et al, 1999) and the resolution of racemic mixtures to produce optically active compounds (Muralidhar et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Lipase Activity in Fermented Oil Seeds of Africa Locust Bean, (Parkia Biglobosa), Castor Seeds (Ricinu Communis) and African Oil Bean (Pentaclethra Macrophylla).

Liman¹,

Egwin²,

Vunchi³

et al. 2010

Nig J Bas App Sci

View full text Add to dashboard Cite

ABSTRACT:The activity of lipase in fermented Africa locust bean, Castor seed, and Africa oil bean was determined. The peak lipase activity for fermented Africa locust bean, Castor seed, and African oil bean were 13.3x10 -3 µmol/sec, 7.2 x10 -3 µmol/sec and 10.6 x10 -3 µmol/sec at day 5, 4 and 4 respectively. The optimum temperature and pH were 30 0 C and 7.0 respectively for all the fermented seeds above. Increasing NaCl concentration decreases the activity of Lipase indicating that NaCl is an inhibitor of lipase. The effect of substrate concentration on lipase activity in the fermenting oil seeds shows the normal Michaelis-Menten curve. The K m for African locust bean is 0.065664mM, Castor seed is 0.067625mM, and African oil bean is 0.075848mM. While the V max are: 15.229x10 -3 µmol/sec, 14.787x10 -3 µmol/sec and 13.184x10 -3 µmol/sec respectively. The findings of this work indicates that fermented African locust bean, Castor seed and Africa oil bean could be a source of lipase for industrial application.

show abstract

Section: Introductionmentioning

confidence: 99%

Lipase Activity in Fermented Oil Seeds of Africa Locust Bean, (Parkia Biglobosa), Castor Seeds (Ricinu Communis) and African Oil Bean (Pentaclethra Macrophylla).

Liman¹,

Egwin²,

Vunchi³

et al. 2010

Nig J Bas App Sci

View full text Add to dashboard Cite

show abstract

“…In addition to their role in synthetic organic chemistry, they find extensive applications in chemical, pharmaceutical, food and leather industries [26,27]. Promising fields for the application of lipases also include the biodegradation of plastics [24] and the resolution of racemic mixtures to produce optically active compounds [46]. These functions of lipases owe to their broad specificity for a wide spectrum of substrates, stability in organic solvents and enantioselectivity [16].…”

Section: Introductionmentioning

confidence: 99%

Optimization of extracellular psychrophilic alkaline lipase produced by marine Pseudomonas sp. (MSI057)

Kiran

Shanmughapriya

Jayalakshmi

et al. 2008

Bioprocess Biosyst Eng

View full text Add to dashboard Cite

An endosymbiotic Pseudomonas sp. (MSI057), which could produce high yields of lipase, was isolated from marine sponge Dendrilla nigra, collected from the peninsular coast of India. Maximum production of enzyme was obtained in minimal medium supplemented with 1% tributyrin. Catabolite repression was observed when the medium was supplemented with readily available carbon sources. The optimum temperature and pH for the enzyme production was 30 degrees C and 9.0, respectively. The enzyme exhibited maximum activity in pH range of 8-9 with an optimum pH 9.0. The activity of purified enzyme was optimum at 37 degrees C and showed 80% activity at 20 degrees C and the enzyme activity decreased dramatically above 50 degrees C. Based on the present findings, the enzyme was characterized as psychrophilic alkaline lipase, which can be developed for industrial applications.

show abstract

“…Biochemical studies on screening lipases from various origins were sufficiently conducted, so that their characteristics such as hydrolysis activity and substrate/positional specificity have been clearly understood [12][13][14][15][16][17][18][19][20][21][22][23]. Several organic solvents never lowering the enzyme activity have been also reported [20][21][22][23][24].…”

Section: Present Status Of the Kinetic Modelmentioning

confidence: 99%

“…Therefore, the above problems can be overcome by this method. Furthermore, the enzymes have substrate and positional specificities [12][13][14][15][16][17], so that the side reactions such as saponification, polymerization and oxidation are prevented to enhance the yield of the desired product.…”

Section: Introductionmentioning

confidence: 99%

Kinetic Model for Triglyceride Hydrolysis Using Lipase: Review

Hermansyah

Wijanarko

2010

MST

View full text Add to dashboard Cite

Triglyceride hydrolysis using lipase has been proposed as a novel method to produce raw materials in food and cosmetic industries such as diacylglycerol, monoacylglycerol, glycerol and fatty acid. In order to design a reactor for utilizing this reaction on industrial scale, constructing a kinetic model is important. Since the substrates are oil and water, the hydrolysis takes place at oil-water interface. Furthermore, the triglyceride has three ester bonds, so that the hydrolysis stepwise proceeds. Thus, the reaction mechanism is very complicated. The difference between the interfacial and bulk concentrations of the enzyme, substrates and products, and the interfacial enzymatic reaction mechanism should be considered in the model.

show abstract

Lipases in racemic resolutions

Cited by 45 publications

References 50 publications

Lipase Activity in Fermented Oil Seeds of Africa Locust Bean, (Parkia Biglobosa), Castor Seeds (Ricinu Communis) and African Oil Bean (Pentaclethra Macrophylla).

Lipase Activity in Fermented Oil Seeds of Africa Locust Bean, (Parkia Biglobosa), Castor Seeds (Ricinu Communis) and African Oil Bean (Pentaclethra Macrophylla).

Optimization of extracellular psychrophilic alkaline lipase produced by marine Pseudomonas sp. (MSI057)

Kinetic Model for Triglyceride Hydrolysis Using Lipase: Review

Contact Info

Product

Resources

About