Lipases for Biofuel Production

Okoro, Oseweuba Valentine; Sun, Zhifa; Birch, John

doi:10.1016/b978-0-08-100596-5.21650-8

Cited by 4 publications

(3 citation statements)

References 48 publications

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“…Moreover, fungal lipases are easily produced in large-scale production, which is more attractive for industrial applications [9]. The utilization of lipases in biofuel production has drawn attention from researchers due to their ability to catalyze with a wide range of oils, including low-grade oil containing a high amount of free fatty acids [10]. Xiao et al [11] reported biodiesel production using a whole-cell biocatalyst from Aspergillus niger, which gave a high biodiesel yield (> 90 %) after 72 h. Li et al [12] reported the utilization of Rhizopus oryzae lipase expressed in Pichia pastoris for enzymatic biodiesel production.…”

Section: Introductionmentioning

confidence: 99%

Purification and Characterization of an Extracellular Lipase Produced by Aspergillus oryzae ST11 as a Potential Catalyst for an Organic Synthesis

et al. 2021

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The lipase producing Aspergillus sp. ST11 was identified by molecular and morphological methods. The primers ITS1/ITS4 were used for amplifying the ITS region. It showed that the strain was grouped with Aspergillus oryzae and Aspergillus flavus (98 % bootstrap value). The colony morphology of Aspergillus sp. ST11 on malt extract agar and Czapek yeast agar showed a characteristic of A. oryzae. Therefore, it was identified as Aspergillus oryzae ST11. The lipase produced by the strain was purified and characterized. The purification steps involved precipitation with chilled acetone and separation by column chromatography, with HiTrap® Q HP and Toyopearl Butyl-650M, respectively. After purification, the lipase activity was increased 13 fold and with 7.9 % yield. Its molecular mass was 25 kDa. The purified lipase was stable at a pH between 5.0 - 8.0 and had optimum activity at pH 7.5. It was stable at 30 °C and had optimum activity at 37 °C. Its activity was promoted in the presence of Mg2+ but it was greatly decreased in the presence of Co2+, Cu2+, Hg2+ and Zn2+. Surfactants (Triton X-100, Tween-80, Tween-20, arabic gum, and sodium dodecyl sulfate) showed negative effects on lipase activity, while inhibitors (PMSF, EDTA, and β-mercaptoethanol) did not reduce the activity significantly. Polar solvents, such as methanol and ethanol, had much negative effect on lipase activity compared to non-polar solvents, such as hexane and isooctane. The concentrated lipase from A. oryzae ST11 was used to catalyze the transesterification and gave the highest bioconversion (90 %) after 24 h. HIGHLIGHTS Extracellular lipase produced by Aspergillus oryzae ST11 could be applied in many applications which is more flexible compared with the use of whole-cell biocatalysts High stability toward a wide range of pH and temperature obtained from this extracellular lipase The concentrated lipase shows the capability to produce the high biodiesel from natural oil GRAPHICAL ABSTRACT

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Section: Introductionmentioning

confidence: 99%

Purification and Characterization of an Extracellular Lipase Produced by Aspergillus oryzae ST11 as a Potential Catalyst for an Organic Synthesis

et al. 2021

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“…Biodiesel is a renewable fuel made from biomass, such as plants (vegetable oils) or animals (animal fat) (dos Santos Silva et al, 2011;Chua et al, 2020;Singh et al, 2020). It is a mixture of methyl or ethyl esters of fatty acids (Tiwari et al, 2018;Zhong et al, 2020), produced by the transesterification and esterification reaction in the presence of a catalyst (Teo et al, 2016;Aguieiras et al, 2017), such as lipases (Ycel et al, 2012;Verdasco-Martín et al, 2016;Okoro et al, 2019;Moreira et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…In fact, lipases are used in different areas, such as biofuel production ( Rodrigues and Fernandez-Lafuente, 2010 ; de Vasconcellos et al, 2018 ; Sahoo et al, 2018 ). Among the renewable fuels, biodiesel stands out as one of the most promising ( Wenlei and Ning, 2009 ; Christopher et al, 2014 ; Okoro et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Lipase From Rhizomucor miehei Immobilized on Magnetic Nanoparticles: Performance in Fatty Acid Ethyl Ester (FAEE) Optimized Production by the Taguchi Method

Moreira

Oliveira

Júnior

et al. 2020

Front. Bioeng. Biotechnol.

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In this communication, it was evaluated the production of fatty acid ethyl ester (FAAE) from the free fatty acids of babassu oil catalyzed by lipase from Rhizomucor miehei (RML) immobilized on magnetic nanoparticles (MNP) coated with 3-aminopropyltriethoxysilane (APTES), Fe 3 O 4 @APTES-RML or RML-MNP for short. MNPs were prepared by co-precipitation coated with 3-aminopropyltriethoxysilane and used as a support to immobilize RML (immobilization yield: 94.7 ± 1.0%; biocatalyst activity: 341.3 ± 1.2 U p−NPB /g), which were also activated with glutaraldehyde and then used to immobilize RML (immobilization yield: 91.9 ± 0.2%; biocatalyst activity: 199.6 ± 3.5 U p−NPB /g). RML-MNP was characterized by X-Ray Powder Diffraction (XRPD), Fourier Transform-Infrared (FTIR) spectroscopy and Scanning Electron Microscope (SEM), proving the incorporation and immobilization of RML on the APTES matrix. In addition, the immobilized biocatalyst presented at 60 • C a half-life 16-19 times greater than that of the soluble lipase in the pH range 5-10. RML and RML-MNP showed higher activity at pH 7; the immobilized enzyme was more active than the free enzyme in the pH range (5-10) analyzed. For the production of fatty acid ethyl ester, under optimal conditions [40 • C, 6 h, 1:1 (FFAs/alcohol)] determined by the Taguchi method, it was possible to obtain conversion of 81.7 ± 0.7% using 5% of RML-MNP.

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Role of microbial lipases in transesterification process for biodiesel production

Bhan

Singh

2020

Environmental Sustainability

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Lipases for Biofuel Production

Cited by 4 publications

References 48 publications

Purification and Characterization of an Extracellular Lipase Produced by Aspergillus oryzae ST11 as a Potential Catalyst for an Organic Synthesis

Purification and Characterization of an Extracellular Lipase Produced by Aspergillus oryzae ST11 as a Potential Catalyst for an Organic Synthesis

Lipase From Rhizomucor miehei Immobilized on Magnetic Nanoparticles: Performance in Fatty Acid Ethyl Ester (FAEE) Optimized Production by the Taguchi Method

Role of microbial lipases in transesterification process for biodiesel production

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