Lipases A and B from the yeast Candida antarctica

Creating Space for Large Secondary Alcohols by Rational Redesign of Candida antarctica Lipase B

“…Initial reaction rates (v) were determined within 5 % conversion, with the alcohols competing as acyl acceptors at known concentrations. From the initial reaction rates and the known substrate concentrations, the relative specificity constants (k cat /K M ) S1 /(k cat /K M ) S2 can be calculated according to Equation (1), where S 1 and S 2 are any of the acyl acceptors. For the alcohols to be compared, the initial reaction rates have to be determined during the same time course.…”

“…[1] Because of its high activity, stability and selectivity in both aqueous and organic solutions, CALB has been well studied and used in numerous applications. [2] CALB was first crystallized in 1994 and was found to belong to the a/b-hydrolase family.…”

Enzymatic Hydrolysis of a Prochiral 3-Substituted Glutarate Ester, an Intermediate in the Synthesis of an NK1/NK2 Dual Antagonist

“…This finding was somewhat unexpected in light of the commonly accepted view that Candida antarctica lipase B is a highly thermostable enzyme capable of tolerating temperatures up to 80°C. [10,17] Under our operating conditions, the immobilized lipase preparation lost 30% of its original activity within the first 18 h. The inactivation of the soluble enzyme was even more pronounced with a half-life of only 15 h at 32°C, pH 8.0.…”

Chemoenzymatic Preparation of Enantiopure Homoadamantyl β‐Amino Acid and β‐Lactam Derivatives

“…A CAL-A (calcium-dependent, thermostable lipase A from Candida antarctica) [18,19] preparation (the lipase was adsorbed on Celite in the presence of sucrose) [20] was earlier found to display excellent (2R)-enantioselectivity for the N-acylation of monocyclic (rings containing 5 ± 8 or 12 carbon atoms) and bicyclic (bicyclo[2.2.1]heptane or -heptene) b-amino esters with 2,2,2-trifluoroethyl esters of butanoic and longer carboxylic acids in ethereal solutions. [5,10] It was essential that the functional groups in these b-amino carboxylates were in the cis, or in the di-endo or di-exo orientation.…”