Lipases

Abstract

Bioengineering is seen as a promising technique for yielding products by exploiting the concept of green chemistry. Amongst various enzymes available from diverse microbial and other sources, lipase is most versatile as it is endowed with the property of performing biochemical reaction in aqueous as well as organic/water‐restricted solvents. Microbial lipases have been expressed in efficient expression vectors to enhance their yields for commercial applications. However, to perform productive and efficient catalysis, involvement of principles of structural engineering of the biocatalyst, reaction conditions and choice of solvents is essential to achieve a higher rate of catalysis. The review revolves around the use of principles of bioengineering to subtly exploit inherent properties of lipases.

Enzymatic Synthesis of Isopropyl Acetate by Immobilized Bacillus cereus Lipase in Organic Medium

“…Environment-friendly biocatalysts are now rapidly substituting the conventional harsh chemical methods for the synthesis of important fatty acid esters used in many chemicals, medicines, cosmetics, and foods [ 1 – 5 ]. The attention towards tremendous use of microbial lipase (triacylglycerol acylhydrolase, EC 3.1.1.3) was exploited in the past decade leading to the easy hydrolysis/synthesis of esters at ambient condition with an advantage of precise selectivity.…”