Lipase, protease, and biofilm as the major virulence factors in staphylococci isolated from acne lesions

Saising, Jongkon; Singdam, Sudarat; Ongsakul, Metta; Voravuthikunchai, Supayang Piyawan

doi:10.5582/bst.2012.v6.4.160

Cited by 31 publications

(21 citation statements)

References 27 publications

(24 reference statements)

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“…Storage of foods such as meat, milk and fish for long periods under refrigeration temperatures has resulted in many quality and shelf life problems for meat and milk products which are related to growth and metabolic activities of psychrotrophic species (Saising et al . ). There was less study in the protease and lipase activities of S. aureus isolated from meat and milk samples.…”

Section: Resultsmentioning

confidence: 97%

“…However, bacterial lipolytic and proteolytic activities are influenced by several factors, such as physiological state of culture, substrate type, pH and temperature (Saising et al . ).…”

Section: Resultsmentioning

confidence: 97%

“…After incubation, the diameter of the clear zone of casein hydrolysis (in millimeters) was measured directly on NA (Saising et al . ).…”

Section: Methodsmentioning

confidence: 97%

“…The presence of clear zones was taken as an indication of positive lipase activity (Saising et al . ).…”

Section: Methodsmentioning

confidence: 97%

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Determination of Some Virulence Factors in Staphylococcus aureus, Enterococcus faecalis and Enterococcus faecium Isolated from Meat and Milk Products

Gündoğan

Ataol

Torlak

2013

Journal of Food Safety

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The aim of this work is to characterize Staphylococcus aureus, Enterococcus faecalis and Enterococcus faecium isolated from meat and milk products in Ankara, Turkey. A total of 40 S. aureus, 55 E. faecalis and 30 E. faecium isolates were isolated from raw calf meat (minced), chicken drumsticks, raw milk, ice cream and white cheese samples. A high proportion of S. aureus isolates had slime formation (80%), biofilm formation (70%), deoxyribonuclease (DNase) activity (53%), hemolytic activity (40%), protease production (80%) and lipase production (45%). The frequency of positive slime formation, DNase activity, hemolysis and protease production for E. faecalis isolates was 36, 5, 7 and 16%, and for E. faecium isolates was 50, 10, 13 and 6%. Neither biofilm formation nor lipase production was detected in Enterococcus isolates. It was found that S. aureus isolates were resistant to penicillin (77%), ampicillin (67%), erythromycin (30%), tetracycline (25%), chloramphenicol (20%) and gentamicin (17%), but they were sensitive to vancomycin and teicoplanin. All Enterococcus spp. isolates were sensitive to ampicillin, chloramphenicol, teicoplanin, tetracycline and vancomycin, but they were resistant to erythromycin (47%), gentamicin (14%) and penicillin (4%). PRACTICAL APPLICATIONS

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Section: Resultsmentioning

confidence: 97%

“…However, bacterial lipolytic and proteolytic activities are influenced by several factors, such as physiological state of culture, substrate type, pH and temperature (Saising et al . ).…”

Section: Resultsmentioning

confidence: 97%

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Determination of Some Virulence Factors in Staphylococcus aureus, Enterococcus faecalis and Enterococcus faecium Isolated from Meat and Milk Products

Gündoğan

Ataol

Torlak

2013

Journal of Food Safety

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“…Several QS-based VFs, such as lipase, protease, and swarming motility, have been found to be associated with staphylococcal pathogenesis (28). Several proteases have been reported to be produced by both health-associated and community-associated S. aureus strains, and some of them have been suggested to have a role in the degree of virulence (19).…”

Section: Discussionmentioning

confidence: 99%

Colostrum Hexasaccharide, a Novel Staphylococcus aureus Quorum-Sensing Inhibitor

Srivastava

Singh

Deepak

et al. 2015

Antimicrob Agents Chemother

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The discovery of quorum-sensing (QS) systems regulating antibiotic resistance and virulence factors (VFs) has afforded a novel opportunity to prevent bacterial pathogenicity. Dietary molecules have been demonstrated to attenuate QS circuits of bacteria. But, to our knowledge, no study exploring the potential of colostrum hexasaccharide (CHS) in regulating QS systems has been published. In this study, we analyzed CHS for inhibiting QS signaling in Staphylococcus aureus. We isolated and characterized CHS from mare colostrum by high-performance thin-layer chromatography (HPTLC), reverse-phase high-performance liquid chromatography evaporative light-scattering detection (RP-HPLC-ELSD), 1 H and 13 C nuclear magnetic resonance (NMR), and electrospray ionization mass spectrometry (ESI-MS). Antibiofilm activity of CHS against S. aureus and its possible interference with bacterial QS systems were determined. The inhibition and eradication potentials of the biofilms were studied by microscopic analyses and quantified by 96-well-microtiter-plate assays. Also, the ability of CHS to interfere in bacterial QS by degrading acyl-homoserine lactones (AHLs), one of the most studied signal molecules for Gram-negative bacteria, was evaluated. The results revealed that CHS exhibited promising inhibitory activities against QS-regulated secretion of VFs, including spreading ability, hemolysis, protease, and lipase activities, when applied at a rate of 5 mg/ml. The results of biofilm experiments indicated that CHS is a strong inhibitor of biofilm formation and also has the ability to eradicate it. The potential of CHS to interfere with bacterial QS systems was also examined by degradation of AHLs. Furthermore, it was documented that CHS decreased antibiotic resistance in S. aureus. The results thus give a lead that mare colostrum can be a promising source for isolating a next-generation antibacterial.

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Enzymology of Microbial Biofilms

Bouaziz¹,

Houfani²,

Baoune³

2022

Ecological Interplays in Microbial Enzymology

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Lipase, protease, and biofilm as the major virulence factors in staphylococci isolated from acne lesions

Cited by 31 publications

References 27 publications

Determination of Some Virulence Factors in Staphylococcus aureus, Enterococcus faecalis and Enterococcus faecium Isolated from Meat and Milk Products

Determination of Some Virulence Factors in Staphylococcus aureus, Enterococcus faecalis and Enterococcus faecium Isolated from Meat and Milk Products

Colostrum Hexasaccharide, a Novel Staphylococcus aureus Quorum-Sensing Inhibitor

Enzymology of Microbial Biofilms

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