Lipase production with free and immobilized cells of cold-adapted yeast Rhodotorula glutinis HL25

Taşkın, Mesut; Ucar, Muhammed Hanifi; Ünver, Yağmur; Kara, Aslı; Özdemir, Mustafa; Örtücü, Serkan

doi:10.1016/j.bcab.2016.08.009

Cited by 28 publications

(15 citation statements)

References 32 publications

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“…from King George Island, the sub‐Antarctic region . Taskin et al reported about a cold adapted Rhodotorula glutinis HL25 which showed highest lipase activity at 20 °C.…”

Section: Discussionmentioning

confidence: 99%

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A cold and organic solvent tolerant lipase produced by Antarctic strain Rhodotorula sp. Y‐23

Maharana

Singh

2018

J Basic Microbiol

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Psychrotolerant yeast Rhodotorula sp. Y-23 was isolated from the sediment core sub-samples of Nella Lake, East Antarctica. Isolate was screened for lipase production using plate assay method followed by submerged fermentation. Production optimization revealed the maximum lipase production by using palmolein oil (5% v/v), pH 8.0 and inoculum size of 2.5% v/v at 15 °C. The potential inducers for lipase were 1% w/v of galactose and KNO , and MnCl (0.1% w/v). Final productions with optimized conditions gave 5.47-fold increase in lipase production. Dialyzed product gave a purification fold of 5.63 with specific activity of 26.83 U mg and 15.67% yields. This lipase was more stable at pH 5.0 and -20 °C whereas more activity was found at pH 8.0 and 35 °C. Stability was more in 50 mM Fe , EDTA-Na (20 mM), sodium deoxycholate (20 mM), H O (1% v/v), and almost all organic solvents (50% v/v). Tolerance capacity at wider range of pH and temperature with having lower K value i.e., 0.08 mg ml and higher V 385.68 U mg at 15 °C make the studied lipase useful for industrial applications. Besides this, the lipase was compatible with commercially available detergents, and its addition to them increases lipid degradation performances making it a potential candidate in detergent formulation.

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“…from King George Island, the sub‐Antarctic region . Taskin et al reported about a cold adapted Rhodotorula glutinis HL25 which showed highest lipase activity at 20 °C.…”

Section: Discussionmentioning

confidence: 99%

“…The present study corroborates with the study of Joseph et al who reported initial pH of 8.0 induces cold active lipase production (1.66 U ml −1 ). Taskin et al reported about a cold adapted Rhodotorula glutinis HL25, which showed a contradict result of getting highest lipase activity at pH 6.0.…”

Section: Discussionmentioning

confidence: 99%

A cold and organic solvent tolerant lipase produced by Antarctic strain Rhodotorula sp. Y‐23

Maharana

Singh

2018

J Basic Microbiol

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“…Yeasts, particularly from the Basidiomycota, are also common in frozen environments (Arendrup, ; Selbmann et al, ; Boetius et al, ; Coleine et al, ), in which water availability can also be limited. In addition to their role in fragile polar and mountain environments, the study of yeasts brings the potential for an industrially attractive low‐temperature enzyme repertoire (Buzzini et al, ; Amaretti et al, ; Morita et al, ; Taskin et al, ; Martorell et al, ). Outside water‐limited environments, yeasts from the Ustilaginomycotina, Saccharomycotina and Pucciniomycotina dominate marine fungal communities (Bass et al, ; Richards et al, , ; Manohar et al, ).…”

Section: The Yeast Lifestylementioning

confidence: 99%

Fungal evolution: major ecological adaptations and evolutionary transitions

2019

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Fungi are a highly diverse group of heterotrophic eukaryotes characterized by the absence of phagotrophy and the presence of a chitinous cell wall. While unicellular fungi are far from rare, part of the evolutionary success of the group resides in their ability to grow indefinitely as a cylindrical multinucleated cell (hypha). Armed with these morphological traits and with an extremely high metabolical diversity, fungi have conquered numerous ecological niches and have shaped a whole world of interactions with other living organisms. Herein we survey the main evolutionary and ecological processes that have guided fungal diversity. We will first review the ecology and evolution of the zoosporic lineages and the process of terrestrialization, as one of the major evolutionary transitions in this kingdom. Several plausible scenarios have been proposed for fungal terrestralization and we here propose a new scenario, which considers icy environments as a transitory niche between water and emerged land. We then focus on exploring the main ecological relationships of Fungi with other organisms (other fungi, protozoans, animals and plants), as well as the origin of adaptations to certain specialized ecological niches within the group (lichens, black fungi and yeasts). Throughout this review we use an evolutionary and comparative-genomics perspective to understand fungal ecological diversity. Finally, we highlight the importance of genome-enabled inferences to envision plausible narratives and scenarios for important transitions.

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“…Several yeasts have been described as lipase producers, and among them species of the genus Candida are the most commercially used ones [31]. However, lipase-producing basidiomycetes species have also been isolated and characterized [2,20,[32][33][34][35]. In accordance with recent reports, in this study most of the strains were able to synthesize lipases and gave positive results in the plate tests ( Table 2 and Figure S1), w hereas, a proteolytic activity was only demonstrated for Cystofilobasidium macerans WUT145 ( Figure S2).…”

Section: Physiological and Biochemical Characteristic Of Yeastsmentioning

confidence: 99%

Carotenoid-Producing Yeasts: Identification and Characteristics of Environmental Isolates with a Valuable Extracellular Enzymatic Activity

et al. 2019

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Sixteen cold-adapted reddish-pigmented yeast strains were obtained from environmental samples. According to the PCR-based detection of classical yeast markers combined with phylogenetic studies, the yeasts belong mainly to the genera Rhodotorula, Sporobolomyces and Cystobasidium, all within the subphylum Pucciniomycotina. All strains produced carotenoids within a 0.25–10.33 mg/L range under non-optimized conditions. Noteworthily, among them, representatives of the Cystobasidium genus were found; of particular value are the strains C. laryngis and C. psychroaquaticum, poorly described in the literature to date. Interestingly, carotenoid production with representatives of Cystobasidium was improved 1.8- to 10-fold at reduced temperature. As expected, most of the isolated yeasts biosynthesized extracellular lipases, but within them also one proteolytic and four cellulolytic strains were revealed. We succeeded in isolating strain Cystofilobasidium macerans WUT145 with extraordinarily high cellulolytic activity at 22°C (66.23 ± 0.15 µmol/mg protein·min) that is described here for the first time. Consequently, a set of yeasts capable of producing both carotenoids and extracellular enzymes was identified. Taking into account those abilities, the strains might be applicable for a development of carotenoids production on an agro-industrial waste, e.g., lignocellulose.

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Lipase production with free and immobilized cells of cold-adapted yeast Rhodotorula glutinis HL25

Cited by 28 publications

References 32 publications

A cold and organic solvent tolerant lipase produced by Antarctic strain Rhodotorula sp. Y‐23

A cold and organic solvent tolerant lipase produced by Antarctic strain Rhodotorula sp. Y‐23

Fungal evolution: major ecological adaptations and evolutionary transitions

Carotenoid-Producing Yeasts: Identification and Characteristics of Environmental Isolates with a Valuable Extracellular Enzymatic Activity

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