Lipase production by Aspergillus terreus using mustard seed oil cake as a carbon source

Sethi, Bijay Kumar; Rout, Jyoti Ranjan; Das, Rajat K.; Nanda, Prativa Kumari; Sahoo, Sabuj

doi:10.1007/s13213-012-0467-y

Cited by 34 publications

(37 citation statements)

References 60 publications

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“…At lower inoculum level, the microbial biomass cannot proliferate quickly; hence, substrate degradation is slow, which subsequently affects the production of metabolites. The inhibitory effect on enzyme production at higher inoculum size might result from the depletion of the nutrients and oxygen available in the growth medium (48)(49)(50)(51).…”

Section: Inoculum Concentrationmentioning

confidence: 99%

Extracellular Alkaline Lipase from a Novel Fungus Curvularia sp. DHE 5: Optimization of Physicochemical Parameters, Partial Purification and Characterization

El-Ghonemy

El-Gamal

Tantawy

et al. 2017

Food Technol. Biotechnol.

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SummaryThirty isolated fungal strains were screened for lipase production using Phenol Red plates, containing tributyrin as lipidic substrate, and a novel fungus identified genetically as Curvularia sp. DHE 5 was found as the most prominent strain. Various agro-industrial substrates were evaluated as inert supports for lipase production in solid-state fermentation. The highest yield of lipase ((83.4±2.2) U/g on dry mass basis) was reported with wheat bran medium after seven days of fermentation at pH=7.0, temperature of 30 °C, 70 % moisture content, inoculum size of 1.27·10 7 spore/mL and 2 % olive oil as an inducer. Supplementation of the medium with 0.05 % KCl as an ion source further increased lipase production to (88.9±1.2) U/g on dry mass basis. The enzyme was partially purified through ammonium sulphate fractionation (40 %) followed by dialysis, and its optimum pH and temperature were reported at 8.0 and 50 °C, respectively, with remarkable pH and thermal stability.

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Section: Inoculum Concentrationmentioning

confidence: 99%

Extracellular Alkaline Lipase from a Novel Fungus Curvularia sp. DHE 5: Optimization of Physicochemical Parameters, Partial Purification and Characterization

El-Ghonemy

El-Gamal

Tantawy

et al. 2017

Food Technol. Biotechnol.

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“…Inoculum preparation: Aspergillus terreus NCFT 4269.10, a pectinase producing strain was used as the working fungus in the study (Sethi et al, 2013a). The master culture was subcultured and one week old PDA slant culture was processed for inoculum build up using potato dextrose broth.…”

Section: Screening Of Fungal Isolates For Pectinase Activitymentioning

confidence: 99%

Biosynthesis of Industrially Relevant Extracellular Pectinase from Aspergillus terreus NCFT 4269.10 Using Orange Peels as Substrate

Sethi¹,

Panda²,

Sahoo³

2014

Asian J. of Biotechnology

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In this study, Aspergillus terreus NCFT4269.10 was selected for the biosynthesis of pectinase after screening from fifteen native fungal strains as the performance of A. terreus was best in pectin hydrolyzing activity (2.75 cm). Aiming at the cost effective fermentation, several agro-industrial residues such as, Pearl Millet (PM), Finger Millet (FM), Orange Peels (OP), Mustard oil Cake (MoC) and Chikling Vetch Peels (CVP) were evaluated for highest pectinase production using Liquid Static Surface Fermentation (LSSF) and Liquid Shaking Fermentation (LShF). Among these substrates, orange peel was found to be the most suitable substrate for optimal pectinase biosynthesis (LSSF: 633.33±57.73 U mLG ) when the culture was grown under static and shaking condition at 30°C for 96 h. Maximum biomass was formed when OP was implemented for fermentation at static condition followed by CVP. But maximum biomass did not always support the enhanced pectinase biosynthesis in most of the cases. Partial purification of pectinase revealed a 3.86 fold purification and 27.63% yield of protein. Fermentation kinetics study revealed that purified enzyme activity yield per gram of substrate was maximum at both LSSF (1866.6 U gdsG 1 ) and LShF (2026.7 U gdsG 1 ) when biosynthesis of pectinase was carried out using OP. Ratio of enzyme yield and biomass were best with OP as compared to the other substrates both in static and shaking fermentation at crude and purified conditions.

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“…Among different tested lipidic carbon sources as a substrate the highest lipase activity was reported with mustard oil lipidic source (29). Different lipidic sources were tested (linseed, soyabean, amla, mustard, coconut, olive, sunflower, til, ricinus).…”

Section: Discussionmentioning

confidence: 99%

Optimization of culture conditions for extracellular fungal lipase production by submerged fermentation process

Shreya

Sharma

et al. 2018

Plant Sci. Today

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The present study aimed to optimize culture conditions for optimal growth and production of extracellular lipase. Lipolytic fungal strain named as S3St2 previously isolated from a petrol pump soil sample of Newai Town was used for optimization study. Among the tested carbohydrate carbon sources, polysaccharide-starch exhibited maximum lipase production (21.25±0.70 IU/ml/min) with highest specific activity (1.47±0.06 U/mg). Lipase activity and specific activity were higher with mustard oil 1 % (v/v) among all lipidic carbon sources. Among inorganic nitrogen source, potassium nitrate was found best inducer of lipase activity, malt extract supported the fungus growth (dry weight of cell pellets was 0.467 g) and exhibited maximum lipase activity among all organic nitrogen sources. Lipase activity was optimum at pH 8.0, indicates alkalophillic nature of production media supports the growth of fungus. Higher lipase activity (27.92±0.87 IU/ml/min) was detected at 28ºC. The incubation time of 5 days was found optimum for maximum lipase production (31.51±0.21 IU/ml/min). Keywords

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Lipase production by Aspergillus terreus using mustard seed oil cake as a carbon source

Cited by 34 publications

References 60 publications

Extracellular Alkaline Lipase from a Novel Fungus Curvularia sp. DHE 5: Optimization of Physicochemical Parameters, Partial Purification and Characterization

Extracellular Alkaline Lipase from a Novel Fungus Curvularia sp. DHE 5: Optimization of Physicochemical Parameters, Partial Purification and Characterization

Biosynthesis of Industrially Relevant Extracellular Pectinase from Aspergillus terreus NCFT 4269.10 Using Orange Peels as Substrate

Optimization of culture conditions for extracellular fungal lipase production by submerged fermentation process

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