Lipase of Bacillus stratosphericus L1: Cloning, expression and characterization

Statistical enhancement of lipase extracellular production byBacillus stratosphericusPSP8 in a batch submerged fermentation process

“…Gricajeva et al . () reported the isolation of B. stratosphericus L1, the extracellular lipase (LipBST) producer, which was cloned and expressed in Escherichia coli . The obtained isolate in this study, B. stratosphericus ‎ PSP8, expressed higher lipase production (47 U ml −1 ) than that of Bacillus pumilus SG2 isolated from food processing industry effluent (38 U ml −1 ; Sangeetha et al .…”

Production, purification and biochemical characterisation of a novel lipase from a newly identified lipolytic bacterium Staphylococcus caprae NCU S6

“…The isolate was further identified by the 16S rRNA gene sequencing method. Then the genomic DNA of NCU (Nanchang University) S6 was obtained from colonies by protease K cleavage method and amplified as described previously 10 . Evolutionary relationship was determined by MEGA version 7.0.…”

An uncharacterized protein from the metagenome with no obvious homology to known lipases shows excellent alkaline lipase properties and potential applications in the detergent industry

“…At present, all lipases that can be added to detergents are alkaline lipases, and their optimum pH is 8.0 -12.0. These lipases are mainly produced by some cultivable microorganisms, such as Bacillus (Niyonzima and More 2014; Gricajeva et al 2016) and Pseudomonas (Phukon et al 2020). Considering the composition of detergents, an ideal lipase for detergent addition should have the following characteristics: high activity, a wide reaction temperature and pH, and strong resistance to alkali, organic solvents, metal ions and other chemical substances (Salihu and Alam 2015).…”