Lipase-Mediated Formation of Peroxyoctanoic Acid Used in Catalytic Epoxidation of α-Pinene from Turpentine Oil

Abstract

Abstract-This work describes the lipase-mediated synthesis of α -pinene oxide at ambient temperature. The immobilized lipase from Pseudomonas aeruginosa is used to generate peroxyoctanoic acid directly from octanoic acid and hydrogen peroxide. The peroxy acid formed is then applied for in situ oxidation of α -pinene. High conversion of α -pinene to α -pinene oxide (approximately 78%) was achieved when using 0,1 g enzim lipase, 6 mmol H 2 O 2 , dan 5 mmol octanoic acid. Various parameters affecting the conversion of α -pinene to α -pinene oxide were studied Index Terms-α-Pinene, P. aeruginosa, octanoic acid.