Lipase-catalyzed synthesis of red pitaya (<i>Hylocereus polyrhizus</i>) seed oil esters for cosmeceutical applications: process optimization using response surface methodology

Abdullah, Abdul Ghani Liew; Gani, Siti Salwa Abd; Hin, Taufiq Yap Yun; Haiyee, Zaibunnisa Abdul; Zaidan, Uswatun Hasanah; Kassim, Mohd Azlan; Halmi, Mohd Izuan Effendi

doi:10.1039/c8ra09418g

Cited by 7 publications

(4 citation statements)

References 28 publications

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“…From Table 5 , the highest OHV was obtained from Experiment 3. Thus, for this reason, Experiment 3 was chosen as the optimum reaction condition [ 46 ]. Therefore, based on CCRD-RSM, an optimised reaction condition with (1:10) mole ratio of (EMO:Glycerol), (0.18%) of catalyst and temperature of 120 °C was suggested, which would produce a polyol with hydroxyl value (OHV) of 306 mg KOH/g.…”

Section: Resultsmentioning

confidence: 99%

Optimisation of Epoxide Ring-Opening Reaction for the Synthesis of Bio-Polyol from Palm Oil Derivative Using Response Surface Methodology

Kamairudin¹,

Hoong

Abdullah

et al. 2021

Molecules

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The development of bio-polyol from vegetable oil and its derivatives is gaining much interest from polyurethane industries and academia. In view of this, the availability of methyl oleate derived from palm oil, which is aimed at biodiesel production, provides an excellent feedstock to produce bio-polyol for polyurethane applications. In this recent study, response surface methodology (RSM) with a combination of central composite rotatable design (CCRD) was used to optimise the reaction parameters in order to obtain a maximised hydroxyl value (OHV). Three reaction parameters were selected, namely the mole ratio of epoxidised methyl oleate (EMO) to glycerol (1:5–1:10), the amount of catalyst loading (0.15–0.55%) and reaction temperature (90–150 °C) on a response variable as the hydroxyl value (OHV). The analysis of variance (ANOVA) indicated that the quadratic model was significant at 98% confidence level with (p-value > 0.0001) with an insignificant lack of fit and the regression coefficient (R2) was 0.9897. The optimum reaction conditions established by the predicted model were: 1:10 mole ratio of EMO to glycerol, 0.18% of catalyst and 120 °C reaction temperature, giving a hydroxyl value (OHV) of 306.190 mg KOH/g for the experimental value and 301.248 mg KOH/g for the predicted value. This result proves that the RSM model is capable of forecasting the relevant response. FTIR analysis was employed to monitor the changes of functional group for each synthesis and the confirmation of this finding was analysed by NMR analysis. The viscosity and average molecular weight (MW) were 513.48 mPa and 491 Da, respectively.

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Section: Resultsmentioning

confidence: 99%

Optimisation of Epoxide Ring-Opening Reaction for the Synthesis of Bio-Polyol from Palm Oil Derivative Using Response Surface Methodology

Kamairudin¹,

Hoong

Abdullah

et al. 2021

Molecules

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“…Lipase (triacylglycerol acylhydrolase E.C.3.1.1.3) is a class of enzymes belonging to the serine hydrolases and is widely known as important biocatalysts. Lipase has been broadly used to catalyze various reactions such as hydrolysis, esterification, interesterification, alcoholysis, aminolysis, acidolysis, and lipolysis [1][2][3][4][5][6][7]. The enzyme is produced by various organisms including animals, plants, bacteria, fungi, and yeast [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Structure elucidation and docking analysis of 5M mutant of T1 lipase Geobacillus zalihae

et al. 2021

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5M mutant lipase was derived through cumulative mutagenesis of amino acid residues (D43E/T118N/E226D/E250L/N304E) of T1 lipase from Geobacillus zalihae. A previous study revealed that cumulative mutations in 5M mutant lipase resulted in decreased thermostability compared to wild-type T1 lipase. Multiple amino acids substitution might cause structural destabilization due to negative cooperation. Hence, the three-dimensional structure of 5M mutant lipase was elucidated to determine the evolution in structural elements caused by amino acids substitution. A suitable crystal for X-ray diffraction was obtained from an optimized formulation containing 0.5 M sodium cacodylate trihydrate, 0.4 M sodium citrate tribasic pH 6.4 and 0.2 M sodium chloride with 2.5 mg/mL protein concentration. The three-dimensional structure of 5M mutant lipase was solved at 2.64 Å with two molecules per asymmetric unit. The detailed analysis of the structure revealed that there was a decrease in the number of molecular interactions, including hydrogen bonds and ion interactions, which are important in maintaining the stability of lipase. This study facilitates understanding of and highlights the importance of hydrogen bonds and ion interactions towards protein stability. Substrate specificity and docking analysis on the open structure of 5M mutant lipase revealed changes in substrate preference. The molecular dynamics simulation of 5M-substrates complexes validated the substrate preference of 5M lipase towards long-chain p-nitrophenyl–esters.

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“…3. In addition, the inclusion of larger 2,4-DP anions generates more pores in the crystallites, increasing the surface area for the intercalated compound [21].…”

Section: Surface Propertiesmentioning

confidence: 99%

Synthesis and Characterization of 2,4-Dichlorophenoxypropanoic Acid (2,4-DP) Herbicide Interleaved into Calcium-Aluminium Layered Double Hydroxide and the Study of Controlled Release Formulation

Bohari

Noor²,

Ghazali³

et al. 2022

Indones. J. Chem.

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The commonly used herbicide in agriculture, namely 2,4-dichlorophenoxypropanoic acid (2,4-DP), is an anionic herbicide used to interleave into the interlayer of calcium-aluminum layered double hydroxide (Ca-Al LDH) employing co-precipitation method to form a new nanohybrid labeled as CAL-2,4DP. The LDH compound serves as a host in supporting the herbicide’s controlled release formulation. The effective interleave was investigated by employing a powder X-Ray Diffraction (XRD) pattern at 0.025 M nanocomposite, which revealed that the basal spacing has increased from 8.0 Å to 23.8 Å. The ATR-FTIR spectra further supported the interleaving, where the nitrate peak (NO−) diminished, and the carboxylate ion (COO−) band appeared at 1653 cm−1. The percentage loading of CAL-2,4DP was 71.26%, calculated from the carbon content in the sample. The BET analysis shows that CAL-2,4DP was a mesoporous material relying on nitrogen-desorption isotherms. The release of 2,4-DP ions into the aqueous solutions followed the order of PO43− > CO32− > Cl− with a percentage of 83, 65, and 30%, respectively. This work indicates the successful interleaving process of the 2,4-DP anion and the potential of CAL-2,4DP as an eco-friendly agrochemical that can be beneficial for farmers in minimizing herbicide usage to the environment.

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Lipase-catalyzed synthesis of red pitaya (Hylocereus polyrhizus) seed oil esters for cosmeceutical applications: process optimization using response surface methodology

Abstract: Optimization of the alcoholysis reaction between red pitaya seed oil and oleyl alcohol catalyzed by Lipozyme RM IM was carried out.

Cited by 7 publications

References 28 publications

Optimisation of Epoxide Ring-Opening Reaction for the Synthesis of Bio-Polyol from Palm Oil Derivative Using Response Surface Methodology

Optimisation of Epoxide Ring-Opening Reaction for the Synthesis of Bio-Polyol from Palm Oil Derivative Using Response Surface Methodology

Structure elucidation and docking analysis of 5M mutant of T1 lipase Geobacillus zalihae

Synthesis and Characterization of 2,4-Dichlorophenoxypropanoic Acid (2,4-DP) Herbicide Interleaved into Calcium-Aluminium Layered Double Hydroxide and the Study of Controlled Release Formulation

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