Lipase-Catalyzed Synthesis of Ethyl Hexanoate in Microemulsion System

Abstract

This paper studied lipase-catalyzed synthesis of ethyl hexanoate in dodecylbenzenesulfonic acid/isooctane/water microemulsion system. The effect of several parameters, such as w0 ([H2O]/[surfactant]) value, reaction time, reaction temperature, oil phase solvent, buffer solution pH value of microemulsion system on the esterification have been investigated. The results showed that the best experimental conditions for catalytic synthesis ethyl hexanoate were as follows: w0 = 4, reaction time 4 h, reaction temperature 40 ºC, buffer solution pH 7. Under these conditions, the conversion of ethyl hexanoate can reach 98.5 %. Lipase-catalyzed synthesis of ethyl hexanoate in dodecylbenzenesulfonic acid inverse microemulsion system has triple mechanism, namely acid catalyzes, microemulsion catalyzes and enzyme catalyzes.

Process Parameters Influence Product Yield and Kinetic Parameters in Lipase Catalysis

“…At higher level, water entered the macrodomain, the interfacial area became larger, ultimately increasing the enzyme activity during CRL-catalyzed esterification in a microemulsion. The microemulsion system converted into an unstable system after optimum water content, reducing interfacial area and ultimately conversion [81].…”