Lipase-catalyzed synthesis of biodiesel in a hydroxyl-functionalized ionic liquid

Fan, Yunchang; Wang, Xin; Zhang, Lina; Li, Jing; Yang, Lei; Gao, Peng-Fu; Zhou, Zhulin

doi:10.1016/j.cherd.2018.01.020

Cited by 32 publications

(15 citation statements)

References 47 publications

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“…With the development of biotechnologies, esterases have been widely used in medicine, food, fine chemicals, and biodiesel industries due to their excellent biological properties [12][13][14]. However, their use in industrial processes has many restrictions under prolonged catalytic reaction conditions or in the presence of organic solvents.…”

Section: Introductionmentioning

confidence: 99%

Cloning, Expression, and Characterization of a Novel Thermostable and Alkaline-stable Esterase from Stenotrophomonas maltophilia OUC_Est10 Catalytically Active in Organic Solvents

Gao

Mao

et al. 2019

Catalysts

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A thermostable and alkaline-stable novel esterase (Est7) was identified through the whole genome sequencing of Stenotrophomonas maltophilia OUC_Est10. The open reading frame of this gene encoded 617 amino acid residues. After heterologous expression in Escherichia coli BL21 (DE3), the purified Est7 was separated as a single protein and presented a molecular mass of 70.6 kDa. Multiple sequence alignment indicated that Est7 had a typical catalytic triad (Ser-Asp-His) and the conserved sequence (GDSL) typical of the family II lipid hydrolase proteins. Est7 showed good stability in alkaline buffers, especially in Tris-HCl buffer at pH 9.0 (residual activity 93.8% after 96 h at 4 °C) and in the medium temperature conditions (residual activity 70.2% after 96 h at 45 °C and pH 8.0). The enzyme also retained higher stability toward several hydrophilic and hydrophobic organic solvents (e.g., after incubation in 100% acetonitrile or in n-hexane the enzyme retained about 97% and 84% of the activity in the absence of organic solvent, respectively). Furthermore, Est7 could catalyze the transesterification reaction of vinylacetate with 2-phenylethanol and cis-3-hexen-1-ol to their corresponding acetate esters in petroleum ether or tert-butyl methyl ether. These results indicate Est7 as a promising biocatalyst for applications of Est7 in non-aqueous media.

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Section: Introductionmentioning

confidence: 99%

Cloning, Expression, and Characterization of a Novel Thermostable and Alkaline-stable Esterase from Stenotrophomonas maltophilia OUC_Est10 Catalytically Active in Organic Solvents

Gao

Mao

et al. 2019

Catalysts

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“…Lipase was dissolved in hydroxyl-functionalized pyrrolidinium based IL ( 5 , Figure 5 ) and applied to transesterification of soybean oil and methanol for biodiesel production in 28.6% aqueous IL [ 72 ]. The enzyme was easily separable from the IL simply by water and acetone wash; however, the recovered enzyme lost some activity.…”

Section: Ionic Liquids and Isolated Enzymesmentioning

confidence: 99%

Applications of Ionic Liquids in Whole-Cell and Isolated Enzyme Biocatalysis

et al. 2021

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Ionic liquids have unique chemical properties that have fascinated scientists in many fields. The effects of adding ionic liquids to biocatalysts are many and varied. The uses of ionic liquids in biocatalysis include improved separations and phase behaviour, reduction in toxicity, and stabilization of protein structures. As the ionic liquid state of the art has progressed, concepts of what can be achieved in biocatalysis using ionic liquids have evolved and more beneficial effects have been discovered. In this review ionic liquids for whole-cell and isolated enzyme biocatalysis will be discussed with an emphasis on the latest developments, and a look to the future.

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“…A yield of 94.4% was achieved, compared with 65.8% when tert ‐butanol was employed as a reaction medium. Meanwhile, Fan et al 55 . used hydroxyl‐functionalized ionic liquid in the methanolysis of soybean oil.…”

Section: Lipase‐mediated Biodiesel Productionmentioning

confidence: 99%

Biodiesel production with enzymatic technology: progress and perspectives

Pasha

Dai

Liu

et al. 2021

Biofuels Bioprod Bioref

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Rapid changes in the global climate resulting from anthropogenic activities and greenhouse gas emissions from fossil resources are encouraging commercial and academic research to seek out new routes for the production of sustainable fuels. Biodiesel, as a renewable fuel, has long been recognized as one of the solutions to meet energy demand in a climate‐constrained world. Although, several routes have been devised for biodiesel production, the enzymatic route has attracted substantial research interest. Here we discuss enzymatic esterification / transesterification technology and its contribution to the green synthesis of biodiesel. This review provides a comprehensive assessment of the fundamentals of enzymatic reaction, such as the impact of different reaction components (lipase, acyl acceptor, substrate, and reaction media) on the process parameters, along with recent developments in improving enzymatic biodiesel production, such as different lipases, combinations of lipases, and two‐step methods. Kinetics and the mechanism of enzymatic reaction when lipase is used as a catalyst are also explained in greater detail. Finally, the opportunities and challenges for the development of potentially sustainable and eco‐friendly enzymatic biodiesel technology are discussed. © 2021 Society of Industrial Chemistry and John Wiley & Sons Ltd.

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Lipase-catalyzed synthesis of biodiesel in a hydroxyl-functionalized ionic liquid

Cited by 32 publications

References 47 publications

Cloning, Expression, and Characterization of a Novel Thermostable and Alkaline-stable Esterase from Stenotrophomonas maltophilia OUC_Est10 Catalytically Active in Organic Solvents

Cloning, Expression, and Characterization of a Novel Thermostable and Alkaline-stable Esterase from Stenotrophomonas maltophilia OUC_Est10 Catalytically Active in Organic Solvents

Applications of Ionic Liquids in Whole-Cell and Isolated Enzyme Biocatalysis

Biodiesel production with enzymatic technology: progress and perspectives

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