Lipase-catalyzed syntheses of monoacylglycerols

Bornscheuer, Uwe T.

doi:10.1016/0141-0229(94)00096-a

Cited by 226 publications

(170 citation statements)

References 92 publications

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“…mono and diacylglycerols are an important class of compounds extensively used as emulsifiers [15]. These are normally produced by alcoholysis of triacylglycerols with glycerol in presence of a metal catalyst at high temperatures of 210-240°C [16].…”

Section: Synthesis Of Partial Glycerides Of Oleic Acid By a Terreus mentioning

confidence: 99%

Optimization of Lipase Production from Aspergillus terreus by Response Surface Methodology and Its Potential for Synthesis of Partial Glycerides Under Solvent Free Conditions

et al. 2010

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Aspergillus terreus produces lipase 7.01 IU/ml in 96 h after optimization by one variable at a time method. Using the significant factors i.e. corn oil (A), sodium nitrate (B), casein (C), agitation rate (D) and incubation period (E) RSM was carried out resulting in 19.65 IU/ml from the combination ?1(A), -1(B), -1(C), ?1(D) and 0(E). The interactions between sodium nitrate, casein and agitation with corn oil were most significant. Scale up of production from 250 ml shake flask to 30 l bioreactor resulted in increased productivity of 0.52 IU/ml/h as against 0.2 IU/ ml/h obtained in shake flasks. This lipase could carryout solvent free synthesis of partial glycerides of oleic acid with 96% efficiency in 12 h.

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Section: Synthesis Of Partial Glycerides Of Oleic Acid By a Terreus mentioning

confidence: 99%

Optimization of Lipase Production from Aspergillus terreus by Response Surface Methodology and Its Potential for Synthesis of Partial Glycerides Under Solvent Free Conditions

et al. 2010

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“…This method compromise with certain major drawbacks includes high-energy consumption, low yield, and poor product quality [13]. Molecular distillation is usually needed for the production of high purity MG [14,15]. Moreover, this process produces environmentally undesirable by-products [6].…”

Section: Synthesis Of Monoglyceridesmentioning

confidence: 99%

“…• The advantages of enzymatic synthesis are higher yields and mild reaction conditions, resulting in products of higher quality and lower energy consumption [15,21].…”

Section: Advantages Of Enzymatic Glycerolysismentioning

confidence: 99%

Progress in Synthesis of Monoglycerides for Use in Food and Pharmaceuticals

et al. 2017

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Glycerides are lipid esters of the glycerol molecule and fatty acids. Their primary function is the storage of energy. Due to its structure and properties, glycerol participates in the formulation or synthesis of many compounds such as food products, cosmetics, pharmaceuticals, liquid detergents. Monoglycerides (MGs) can be formed by both industrial chemical glycerolysis and biological or enzymatic processes. Chemical glycerolysis bring issues of low MGs yield, high operating temperature, formation of undesirable by-products and high energy consumption. On the other hand enzymatic processes have advantages of mild reaction conditions and high purity of MGs. But, several purification steps are required to obtain food or pharmaceutical grade MG, such as neutralization of the reaction media and discoloration followed by expensive molecular distillation. The purpose of this article is to review the main challenges in the synthesis of MGs from triglycerides (TGs) contained in the various fixed oils and application thereof in the food and pharmaceuticals.

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“…These chemical and physical processes are not suitable for heat-sensitive oils and fats rich in polyunsaturated fatty acids such as fish oils. Due to the disadvantages of conventional glycerolysis, the enzymatic process is an attractive alternative method for polyunsaturated MAG production because the reaction can be carried out under mild conditions (Bornscheuer, 1995).…”

Section: Introductionmentioning

confidence: 99%

Glycerolysis of sardine oil catalyzed by a water dependent lipase in different tert-alcohols as reaction medium

Solaesa¹,

Sanz²,

Melgosa³

et al. 2015

Grasas y Aceites

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SUMMARY:The production of monoacylglycerol rich in polyunsaturated fatty acids (PUFA) via enzymatic glycerolysis of sardine oil in a homogeneous system was evaluated. Reactions were conducted in two different tert-alcohols. Based on the phase equilibrium data, the amount of solvent added to create a homogeneous system has been calculated and optimized. The immobilized lipase used in this work was Lipozyme RM IM from Rhizomucor miehei, a water dependent lipase. The amount of water added as well as other reaction parameters were studied to evaluate the optimum conditions for monoacylglycerol obtencion. An initial reactant mole ratio glycerol to sardine oil 3:1, 12 wt% of water based on glycerol content and 10 wt% of lipase loading (based on weight of reactants), achieved a MAG yield of around 70%, with nearly 28 wt% PUFA, with low free fatty acid content (lower than 18 wt%).KEYWORDS: Fish oil; Glycerolysis; Lipase; Monoacylglycerol; Rhizomucor miehei; Tert-alcohols RESUMEN: Glicerolisis de aceite de sardina catalizada por una lipasa dependiente de agua en diferentes tert-alcoholes como medio de reacción. En este trabajo se ha estudiado la producción de monoacilglicéridos, ricos en ácidos grasos poliinsaturados (AGPI), mediante glicerolisis enzimática de aceite de sardina. La reacción se ha llevado a cabo en dos tert-alcoholes para conseguir de esta forma un medio homogéneo de reacción. La cantidad de disolvente añadida al medio de reacción se ha optimizado y calculado en base al equilibrio de fases de los componentes del sistema. La lipasa empleada como biocatalizador ha sido la enzima inmovilizada Lipozyme RM IM de Rhizomucor miehei, una lipasa dependiente de agua. Se ha estudiado el efecto de distintos parámetros cinéticos, así como de la cantidad de agua añadida al medio de reacción, en la producción de monoacilglicéridos. De los resultados obtenidos, se puede concluir que, para una relación molar inicial de reactantes glicerol:aceite de sardina de 3:1, un 12 % en peso de agua en base al glicerol y un 10 % en peso de lipasa, en base al peso de reactantes; se puede llegar a conseguir un rendimiento en monoacilglicéridos alrededor del 70 % en peso, con casi un 28 % en peso de AGPI y un bajo contenido en ácidos grasos libres (menor del 18 % en peso).

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Lipase-catalyzed syntheses of monoacylglycerols

Cited by 226 publications

References 92 publications

Optimization of Lipase Production from Aspergillus terreus by Response Surface Methodology and Its Potential for Synthesis of Partial Glycerides Under Solvent Free Conditions

Optimization of Lipase Production from Aspergillus terreus by Response Surface Methodology and Its Potential for Synthesis of Partial Glycerides Under Solvent Free Conditions

Progress in Synthesis of Monoglycerides for Use in Food and Pharmaceuticals

Glycerolysis of sardine oil catalyzed by a water dependent lipase in different tert-alcohols as reaction medium

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