Lipase-catalyzed incorporation of docosahexaenoic acid (DHA) into borage oil: optimization using response surface methodology

Senanayake, S.P.; Shahidi, Fereidoon

doi:10.1016/s0308-8146(01)00311-9

Cited by 103 publications

(57 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Baş and Boyacı [24] reported that the second-order equation obtained from RSM did not show the interactive effects of pH and substrate concentration on the initial reaction rate of the enzymatic reaction in their study. Similar findings were reported in the lipase catalysis and optimization studies of borage oil: that there was no significant interaction among the variables considered using RSM [28].…”

Section: Glucose (G/l)supporting

confidence: 86%

“…Tesfaw and Assefa [46] also confirm the importance of the finding that lower inoculum size reduces the cost of production in ethanol fermentation. The mutually significant relationship between inoculum size and temperature was due to the use of a commercial strain (Anchor Instant Yeast) which operated within the specified temperature range (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35) • C) in the design of the experiment. …”

Section: Rsm Modeling Results For Ethanol Productionmentioning

confidence: 99%

“…It is clear, however, that most of the critical nutrients selected for optimization may be redundant, as they are already contained in adequate quantities in CSL [26]; eliminating them may significantly improve the economics of the process, especially on an industrial scale. While the applicability of RSM to optimization studies has been demonstrated successfully [22,25,27], studies have also shown that its predictive capability may be limited [24,28]. Baş and Boyacı [24] reported that the second-order equation obtained from RSM did not show the interactive effects of pH and substrate concentration on the initial reaction rate of the enzymatic reaction in their study.…”

Section: Glucose (G/l)mentioning

confidence: 99%

See 2 more Smart Citations

Optimization of Corn Steep Liquor Dosage and Other Fermentation Parameters for Ethanol Production by Saccharomyces cerevisiae Type 1 and Anchor Instant Yeast

2018

View full text Add to dashboard Cite

Abstract:Bioethanol production has seen an increasing trend in research recently, with a focus on increasing its economic viability. The aim of this study is to develop a low-cost fermentation medium with a minimum of redundant nutritional supplements, thereby minimizing the costs associated with nutritional supplements and seed production. Corn steep liquor (CSL) in glucose fermentation by Saccharomyces Type 1 (ST1) strain and Anchor Instant Yeast (AIY), which are low-cost media, is used as a replacement for yeast extract (YE). The fermentation process parameters were optimized using artificial neural networks (ANN) and the response surface method (RSM). The study shows that for CSL, maximum average ethanol concentrations of 41.92 and 45.16 g/L, representing 82% and 88% of the theoretical yield, were obtained after 36 h of fermentation in a shake flask for ST1 and AIY, respectively. For YE, ethanol concentrations equivalent to 86% and 88% of theoretical yield were obtained with ST1 and AIY, respectively after 48 h. Although ANN better predicted the responses compared to RSM, optimum conditions were better predicted by RSM. This study shows that corn steep liquor is an inexpensive potential nutrient that may have significant cost implications for commercial ethanol production.

show abstract

Section: Glucose (G/l)supporting

confidence: 86%

Section: Rsm Modeling Results For Ethanol Productionmentioning

confidence: 99%

Section: Glucose (G/l)mentioning

confidence: 99%

See 1 more Smart Citation

Optimization of Corn Steep Liquor Dosage and Other Fermentation Parameters for Ethanol Production by Saccharomyces cerevisiae Type 1 and Anchor Instant Yeast

2018

View full text Add to dashboard Cite

show abstract

“…Furthermore, the products of Borage are used for the treatment of bronchitis, palpitations, cramps and diarrhea (16)(17)(18). This plant can be also considered useful for the treatment of diseases such as premenstrual pain, multiple sclerosis, rheumatoid arthritis and asthma (19)(20)(21). There is a variety of animal tests for the investigation of anxiolytic effects of substances (22).…”

Section: Introductionmentioning

confidence: 99%

Anxiolytic Effect of Borago officinalis (Boraginaceae) Extract in Male Rats

Komaki‬¹,

Rasouli²,

Shahidi³

2015

Avicenna J Neuro Psych Physio

View full text Add to dashboard Cite

Background: Medicinal plants with natural antioxidants have been shown to be beneficial in a variety of complications such as anxiety. The elevated plus-maze (EPM) is one of the most widely used models to assess anxiety in small rodents. Objectives: This study was designed to characterize the anxiolytic-like activity of Borago officinalis (Linnaeus, family Boraginaceae) or Borage flowers extract, using an EPM test. Materials and Methods: Male Wistar rats weighing 220-250 grams were used in the present study. Thirty minutes after an intraperitoneal (IP) injection of the Borage extract (50, 100, 200 mg/kg) or saline, each animal was placed in the EPM. Animal behaviors in the experimental sessions were recorded by a video camera located above the maze, interfaced with a monitor and a computer in an adjacent room. The time spent in the open arms, the percentage of entries into the open arms of the EPM and the numbers of entries into the closed arms were recorded for five minutes. Results: Statistical analysis indicated that acute IP injection of Borage extract before an EPM trial significantly increased the time spent in open arms and percentage of open arms entries. Whereas, the extract had no effect on the number of closed arm entries. Conclusions: Our results demonstrated that injection of Borage extract might have an anxiolytic profile in rats. However, the exact mechanism (s) related to the active compound (s) in Borage extract should be elucidated in future studies.

show abstract

“…RSM also is a faster and more economical method for gathering optimizing research results than one-variable-at-a-time. RSM has been used to optimize enzymatic esterification reactions in organic solvent (Uosukainen et al, 1999) as well as to optimize biochemical and biotechnological processes to food system (Cacace & Mizza, 2003b;Senanayake & Shahidi, 2002;Telez-Luis et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Synthesis of esterified solid fat from fractionated rapeseed oil and palm stearin modeling by response surface methodology

Алим

Wessman

Dutta

2012

J Bangladesh Agric Univ

View full text Add to dashboard Cite

The main purpose of the study was to produce esterified solid fat (ESF) from fractionated rapeseed oil and palm stearin through lipase-catalyzed reaction. The response surface methodology (RSM) was applied for optimization of three reaction factors such as substrate mole ratio, enzyme percent, and reaction time. The design was adequate and reproducible due to satisfactory levels of coefficient of determination (R 2 , 0.98) for both cases and coefficient variation (CV, 4.06 for SFC at 10 °C and 7.95 for SFC at 30 °C, respectively). The substrate mole ratio was the significant term for affecting the response of SFC (P<0.05) rather than reaction time and enzyme percent. Based on ridge analysis, the production of ESF with SFC 51.48 ±0.94 % at 10 ºC would be predicted by the combinations of optimized 24.07 h reaction time, 10.66 % enzyme and 1: 1.52 substrate mole ratios. On the other hand, the SFC of 21.44±0.83 at 30 ºC would be predicted by the combinations of optimized 24.37 h reaction time, 10.23 % enzyme and 1: 1.5 substrate mole ratios. The ESF contained mainly of palmitic (45.1%), oleic (40.8%), linoleic (5.6%) and stearic (4.5%) acids, respectively. The total sterol and tocopherol contents of ESF were 243.27 mg/100g and 19.26 mg/100 g, respectively. Therefore, these results in this study suggested that RSM can be used to optimize the lipase-catalyzed synthesis of ESF with suitable physical characteristics.

show abstract

Lipase-catalyzed incorporation of docosahexaenoic acid (DHA) into borage oil: optimization using response surface methodology

Cited by 103 publications

References 16 publications

Optimization of Corn Steep Liquor Dosage and Other Fermentation Parameters for Ethanol Production by Saccharomyces cerevisiae Type 1 and Anchor Instant Yeast

Optimization of Corn Steep Liquor Dosage and Other Fermentation Parameters for Ethanol Production by Saccharomyces cerevisiae Type 1 and Anchor Instant Yeast

Anxiolytic Effect of Borago officinalis (Boraginaceae) Extract in Male Rats

Synthesis of esterified solid fat from fractionated rapeseed oil and palm stearin modeling by response surface methodology

Contact Info

Product

Resources

About