Lipase catalyzed esterification of formic acid in solvent and solvent-free systems

Abstract

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Solvent-free esterifications mediated by immobilized lipases: a review from thermodynamic and kinetic perspectives

“…However, if the biocatalyst loading increases, the availability of more biocatalyst in the system will increase the reaction rate and may reduce the inhibition effects. This effect can be seen in many different studies (6,17,27,45,52,58,76,136,161) that observed a positive correlation between conversion results and biocatalyst loadings in a given molar ratio within a limited interval of time. As already discussed, biocatalyst loading (the quantity of immobilized lipase in the system) does not affect the equilibrium position, but these observations are related to kinetics in two different ways -the occurrence of some level of enzymatic inhibition and/or insufficient time to reach the equilibrium.…”

“…Kuperkar and co-workers (2014) (45) found the same when compared the synthesis of isopropyl palmitate in a solvent-free system with the study of Varma & Madras (157) that synthesized the ester in the presence of supercritical CO 2 . Aljawish et al (2019) (136) found a comparable yield between the enzymatic synthesis of butyl and octyl formate in acetonitrile and solvent-free conditions, although using different molar ratios of acid and alcohol in both cases. It has been shown how the role of the cosolvents may be different depending on the immobilization support.…”

Simplified Method to Optimize Enzymatic Esters Syntheses in Solvent-Free Systems: Validation Using Literature and Experimental Data

“…Immobilized lipases have been utilized successfully for esterification reactions in SFS [4][5][6][7][8][9]. Enzyme immobilization enables enzyme recovery and reuse, associated with the possibility of improving enzyme stability, activity, selectivity, or specificity [10][11][12].…”

“…The classical approach of evaluating independent variables once a time is still commonly used for enzymatic esterification reaction optimization, even in recent studies [7][8][9][53][54][55][56]. However, it has been shown that correlation among some of the studied variables makes the independent optimization incomplete, and, thus, statistical tools, such as response surface methodology (RSM), became popular for optimization studies [49,[57][58][59][60][61][62][63][64].…”

Effect of Cross-Linked Enzyme Aggregates Strategy on Characterization of sn-1,3 Extracellular Lipase from Aspergillus Niger GZUF36

“…Lipase (EC 3.1.1.3), a multipurpose hydrolase [1], hydrolyzes triglycerides to produce diglycerides, fatty acids, glycerol, and monoglycerides and catalyzes various reactions, such as hydrolysis, ester synthesis, acidolysis, and alcoholysis [2]. In general, the conformation of lipase is related to the movement of the lid on the active center of the lipase.…”