Lipase and protease extraction from activated sludge

Gessesse, Amare; Dueholm, T.D.; Petersen, Steffen B.; Nielsen, Per Halkjær

doi:10.1016/s0043-1354(03)00241-0

Cited by 132 publications

(72 citation statements)

References 16 publications

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“…DTT or Cleland's reagent, is commonly used as a thiol group protectant 56) or protein modulator. It confers protective effects against oxidation, which can inactivate the catalytic sites for the protease.…”

Section: Methodsmentioning

confidence: 99%

Optimization of Protease Extraction from Horse Mango (Mangifera foetidaLour) Kernels by a Response Surface Methodology

Ahmad

Liew

Yarmo

et al. 2012

Bioscience, Biotechnology, and Biochemistry

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Section: Methodsmentioning

confidence: 99%

Optimization of Protease Extraction from Horse Mango (Mangifera foetidaLour) Kernels by a Response Surface Methodology

Ahmad

Liew

Yarmo

et al. 2012

Bioscience, Biotechnology, and Biochemistry

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“…In certain interesting studies, extraction of lipases and proteases from activated sludge using the nonionic detergent Triton X-100, EDTA, and cation exchange resin has been attempted (Gessesse et al 2003). In other experiments, the filamentous bacterium Microthrix parvicella has been demonstrated to be a specialized lipid consumer, being able to take up LCFA under anaerobic conditions and subsequent usage of the stored material for growth when nitrate or oxygen is available as electron acceptors (Nielsen et al 2002).…”

Section: The Role Of Lipases In Activated Sludge Systemsmentioning

confidence: 99%

An insight into microbial lipases and their environmental facet

Kanmani¹,

Aravind²,

Kumaresan³

2014

Int. J. Environ. Sci. Technol.

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Lipases are serine hydrolases that catalyze the hydrolysis and synthesis of esters formed from glycerol and long-chain fatty acids, by acting at the oil-water interface. Lipases from microbial sources have received heightened attention for an array of industrial applications, and these enzymes have been well exploited in the environmental sector as well. In this article, we present an overview of microbial lipase, including the microorganisms from which it could be produced; the application of recombinant DNA technology tools to produce lipase with enhanced properties, the effective use of waste materials as substrates for lipase production; the usage of statistical tools to efficiently optimize the production medium; lipase purification strategies; and the immobilization of the enzyme on a variety of support materials. The next section of the article provides a gist of its application in diversified spheres and focusses exclusively on the environmentally relevant ones. Lipasecatalyzed esterification, transesterification, and interesterification reactions, an emerging area of green chemistry; lipase-mediated in vitro biopolymer synthesis and degradation; and the application of lipase for remediating fat and oil constituents in wastewater are dealt with in-depth. When its full potential is harnessed, the enzyme could play a pivotal role in environmental management.

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“…Functional affiliation related to higher methane production after sludge pretreatment Degradation of the highly organic polymers represents the first and overall rate-limiting step for the mineralization of organic matter in activated sludge and anaerobic digested sludge treatment systems [59][60][61]. In our work, the studied pretreatment involved alteration of pH, which is an important parameter affecting both bacterial activity and metabolite pathways.…”

Section: Methane Production Via Acetoclastic and Hydrogenotrophic Patmentioning

confidence: 99%

Bacterial Consortium and Axenic Cultures Isolated from Activated Sewage Sludge for Biodegradation of Imidazolium-based Ionic Liquid

2016

Environmental Engineering and Activated Sludge Processes

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Background: Understanding the effects of pretreatment on anaerobic digestion of sludge waste from wastewater treatment plants is becoming increasingly important, as impetus moves towards the utilization of sludge for renewable energy production. Although the field of sludge pretreatment has progressed significantly over the past decade, critical questions concerning the underlying microbial interactions remain unanswered. In this study, a metagenomic approach was adopted to investigate the microbial composition and gene content contributing to enhanced biogas production from sludge subjected to a novel pretreatment method (maintaining pH at 10 for 8 days) compared to other documented methods (ultrasonic, thermal and thermal-alkaline).

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Lipase and protease extraction from activated sludge

Cited by 132 publications

References 16 publications

Optimization of Protease Extraction from Horse Mango (Mangifera foetidaLour) Kernels by a Response Surface Methodology

Optimization of Protease Extraction from Horse Mango (Mangifera foetidaLour) Kernels by a Response Surface Methodology

An insight into microbial lipases and their environmental facet

Bacterial Consortium and Axenic Cultures Isolated from Activated Sewage Sludge for Biodegradation of Imidazolium-based Ionic Liquid

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