Lipase activity in Streptomycetes

Large, K.P.; Mirjalili, Noushin; Peacock, M.; Zormpaidis, Vassilios; Walsh, Marie K.; Cavanagh, M.E.; Leadlay, Peter F.; Ison, A. P.

doi:10.1016/s0141-0229(99)00080-0

Cited by 30 publications

(26 citation statements)

References 23 publications

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“…The lipase production was accompanied by an increase in the medium pH for all cultures. The pH of the culture broth became alkaline reaching to 8.9 at 96 h when the volumetric lipase activity was the highest and stayed stable between 8.6 and 8.7 for 168 h. These trends of stable biomass and pH when lipase production was highest are similar to those observed for S. clavuligerus [31].…”

Section: Discussionsupporting

confidence: 76%

“…3). The optimal temperature determined for maximum lipase synthesis and biomass production by A. mediterranei DSM 43304 (28°C) is comparable to those reported for actinomycetes such as S. erythraea and S. clavuligerus [31]. The initial pH of the growth medium is important for lipase production [54].…”

Section: Discussionsupporting

confidence: 65%

“…Lipase activities associated with the cells of different Streptomyces sp. were induced and enhanced by the presence of a lipid substrate in the fermentation medium [31]. In the present investigation, all actinomycete strains showed the presence of lipase activity when their culture supernatants were tested with p-NPP, a chromogenic substrate specific for lipases (Table 1).…”

Section: Discussionmentioning

confidence: 52%

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Influence of cultivation conditions on the production of a thermostable extracellular lipase from Amycolatopsis mediterranei DSM 43304

Dheeman¹,

Frias²,

Henehan³

2009

J Ind Microbiol Biotechnol

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23Among several lipase producing actinomycete strains screened, Amycolatopsis 24 mediterranei DSM 43304 was found to produce a thermostable, extracellular lipase. 25Culture condition and nutrient source modification studies involving carbon sources, 26 nitrogen sources, incubation temperature and medium pH were carried out. Lipase activity 27 of 1.37 ± 0.103 IU/ml of culture medium was obtained in 96 h at 28 °C and pH 7.5 using 28 linseed oil and fructose as carbon sources and a combination of phytone peptone and yeast 29 extract (5:1) as nitrogen sources. In optimal culture conditions the lipase activity was 30 enhanced 12-fold with a 2-fold increase in lipase specific activity. The lipase showed 31 maximum activity at 60 °C and pH 8.

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Section: Discussionsupporting

confidence: 76%

Section: Discussionsupporting

confidence: 65%

Section: Discussionmentioning

confidence: 52%

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Influence of cultivation conditions on the production of a thermostable extracellular lipase from Amycolatopsis mediterranei DSM 43304

Dheeman¹,

Frias²,

Henehan³

2009

J Ind Microbiol Biotechnol

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“…The major factor for the expression of lipase activity has always been reported as the carbon source, since lipases are mostly inducible enzymes and sometimes constitutive, although their role in lipase synthesis and stimulation is poorly understood (Large et al, 1999). Among the various oils used in the present study, maximum lipase production was achieved with castor oil followed by olive oil and mustard oil.…”

Section: Discussionmentioning

confidence: 71%

Enhanced Production of Extracellular Alkaline Lipase by an Improved Strain of <i>Pseudomonas aeruginosa</i> MTCC 10,055

Bisht¹,

Yadav²,

Darmwal³

2012

American Journal of Applied Sciences

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Problem statement: Lipases are industrially important enzymes having applications in numerous industries. For easy commercialization it is necessary to produce lipases at industrial level which could be achieved by strain improvement and medium formulation. Approach: In the present study strain improvement of Pseudomonas aeruginosa MTCC 10,055 was done by chemical mutagenesis using mutagen 4-nitroquinoline1-oxide for alkaline lipase production. Different fermentation parameters affecting lipase production were optimized using one-variable-at-a-time approach. Results: The selected mutant (M-05) exhibited 3.6-fold higher productivity over wild type. Maximum alkaline lipase was produced when culture was incubated at 35°C with initial medium pH 9.0 in 28 h with inoculum density 0.5% (v/v) (Abs 610 -1.0). Supplementation of production medium with combination of castor oil and starch as carbon source and Triton-X-100 as surfactant significantly influenced the alkaline lipase production. The composition of fully optimized medium was determined to be (g L gum arabic, 5.0; Triton-X-100, 1.0. An overall 14-fold enhanced production was achieved after complete medium optimization. Conclusion/Recommendations: The improved strain was capable to produce high titer of alkaline lipase at flask level, which can be examined at fermentor level to obtain sufficient enzyme yield to meet the world wide industrial demand.

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“…Lipases are usually secreted out in the culture medium; although there are a few reports of the presence of intracellular lipases as well as cell bound lipases (Large et al, 1999). The onset of lipase production is organism-specific, but, in general, it is released during late logarithmic or stationary phases.…”

Section: Production Of Lipasesmentioning

confidence: 99%

Lipases in Environmental Management

Bhavani¹,

Saritha²

2017

Int.J.Curr.Res.Aca.Rev.

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Article InfoExtra cellular lipase producing bacteria were isolated from different oil mill soils and screened on tributyrin and spirit blue agar medium. Lipases have become an integral part of the modern food industry. The use of enzymes to improve the traditional chemical processes of food manufacture. Lipases have been used for the degradation of wastewater contaminants such as olive oil from oil mills. The microbial treatment of waste from fast-food restaurants for the removal of fats, oils and greases. They cultivated pure and mixed microbial flora known to produce lipases and other enzymes. Acinetobacter sp. was the most effective of the pure cultures, typically degrading 60-65% of the fatty material.

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Lipase activity in Streptomycetes

Cited by 30 publications

References 23 publications

Influence of cultivation conditions on the production of a thermostable extracellular lipase from Amycolatopsis mediterranei DSM 43304

Influence of cultivation conditions on the production of a thermostable extracellular lipase from Amycolatopsis mediterranei DSM 43304

Enhanced Production of Extracellular Alkaline Lipase by an Improved Strain of <i>Pseudomonas aeruginosa</i> MTCC 10,055

Lipases in Environmental Management

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