Lipase activity in biphasic media: Why interfacial area is a significant parameter?

Saktaweewong, Sunan; Phinyocheep, Pranee; Ulmer, Christoph; Marie, Emmanuelle; Durand, Alain; Inprakhon, Pranee

doi:10.1016/j.molcatb.2011.01.013

Cited by 25 publications

(24 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As many substrates are poorly water-soluble, while enzymes naturally require aqueous environments, some authors have attempted the use of enzymes in biphasic aqueous-organic systems. [45] In this context, examples of lipases favoring the interfacial hydrolysis of triglycerides in w/o emulsions have been reported. Most studies deal with the immobilization of the lipases at the w/o interface assisted by surfactants.…”

Section: Enzymes Located At the L/l Interfacementioning

confidence: 99%

Pickering Interfacial Catalysis for Biphasic Systems: From Emulsion Design to Green Reactions

Leclercq²,

et al. 2015

View full text Add to dashboard Cite

Pickering emulsions are surfactant-free dispersions of two immiscible fluids that are kinetically stabilized by colloidal particles. For ecological reasons, these systems have undergone a resurgence of interest to mitigate the use of synthetic surfactants and solvents. Moreover, the use of colloidal particles as stabilizers provides emulsions with original properties compared to surfactant-stabilized emulsions, microemulsions, and micellar systems. Despite these specific advantages, the application of Pickering emulsions to catalysis has been rarely explored. This Minireview describes very recent examples of hybrid and composite amphiphilic materials for the design of interfacial catalysts in Pickering emulsions with special emphasis on their assets and challenges for industrially relevant biphasic reactions in fine chemistry, biofuel upgrading, and depollution.

show abstract

Section: Enzymes Located At the L/l Interfacementioning

confidence: 99%

Pickering Interfacial Catalysis for Biphasic Systems: From Emulsion Design to Green Reactions

Leclercq²,

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Next, the effects of the specific interfacial area ( S ) and interfacial enzyme concentration ( E i ) of an o/w emulsion on the lipase activity were evaluated. S is defined as the total interfacial area divided by the overall volume of the biphasic medium [m 2 L −1 ] . E i is calculated as the amount of adsorbed enzyme of the interface divided by the total interfacial area of the emulsion [g m −2 ], which means that a linear relation exists between E i and inverse S .…”

Section: Resultsmentioning

confidence: 99%

Amphiphilic Carbonaceous Microsphere‐Stabilized Oil‐in‐Water Pickering Emulsions and Their Applications in Enzyme Catalysis

et al. 2016

View full text Add to dashboard Cite

An oil‐in‐water Pickering emulsion system stabilized by amphiphilic porous carbonaceous microspheres was prepared and successfully used in enzyme‐catalyzed hydrolysis. The control of the emulsion droplet size and size distribution were investigated by adjusting the particle content and the dispersed phase volume fraction. More than 99 % of the lipase was adsorbed at the interface of the emulsion droplets and showed excellent stability and activity. The size of the emulsion droplets exerted two opposite influences on the catalytic efficiency of the enzyme: increasing the rate of the process by providing a large interfacial area, thereby overcoming mass transfer limitations, and reducing the rate of the process by decreasing the interfacial enzyme concentration. A high hydrolysis yield (up to 100 %) was obtained after 2 h. This study shows the great potential and advantages of using an oil‐in‐water Pickering emulsion system for enzyme‐catalyzed reactions.

show abstract

“…29 Além disso, elevadas concentrações de óleo aumentam a concentração de ácidos graxos na interface óleo/água que promove mudanças do estado de ionização da enzima na qual afeta a sua atividade catalítica e seletividade. 33 A variação da concentração de tampão de 100 para 200 mM também foi verificada no processo de hidrólise do óleo, conforme mostrado na Figura 2b. Com o aumento da concentração de tampão é esperado um aumento da porcentagem da hidrólise devido à menor variação do pH no meio de reação e menor agregação das gotas de óleo.…”

Section: Resultsunclassified

Produção de concentrados de ácidos graxos por hidrólise de óleos vegetais mediada por lipase vegetal

Coelho¹,

Santos²,

Domingues³

et al. 2013

Quím. Nova

View full text Add to dashboard Cite

Recebido em 16/1/13; aceito em 8/5/13; publicado na web em 10/6/13 PRODUCTION OF CONCENTRATED FATTY ACIDS BY HYDROLYSIS OF VEGETABLE OILS CATALYZED BY PLANT LIPASE. The aim of this work was to verify the ability of enzymatic crude extract from dormant castor bean seeds to yield concentrated fatty acids by hydrolysis of polyunsaturated vegetable oils such as corn and sunflower. The enzymatic extract exhibited higher activity towards corn oil, which was selected for further studies to determine optimum hydrolysis conditions by factorial design. Maximum hydrolysis percentage (≈84%) was reached at 60% wt. oil:buffer acetate 100 mM pH 4.5, 33 °C and 5.0% wt. of crude extract after 70 min of reaction. These results suggest that the use of low-cost lipase from castor bean seeds has potential for oil hydrolysis.Keywords: oil hydrolysis; factorial design; plant lipase. [1][2][3] Estes números referem-se apenas à produção dos principais óleos e gorduras como soja, algodão, amendoim, girassol, colza, gergelim, milho, oliva, palma, coco, linhaça e mamona, e as gorduras de fonte animal como manteiga, sebo e peixe. 3 A modificação química destes óleos e gorduras mediada por catalisadores químicos é normalmente realizada em elevadas temperaturas e pressão (250 °C e 50 atm). Geralmente, estes processos fornecem produtos de composição química mista e/ou contaminada devido à ocorrência de reações indesejáveis como oxidação, desidratação e interesterificação que requerem etapas posteriores de purificação. Neste contexto, a modificação de óleos e gorduras catalisada por lipases vem se apresentando como uma alternativa atrativa para a indústria, principalmente quando são consideradas algumas das vantagens desta rota como maior rendimento do processo, menor consumo de energia, redução do teor de resíduos e introdução de rotas mais acessíveis de produção. [4][5][6][7][8][9][10] Lipases (glicerol éster hidrolases -E.C. 3.1.1.3) são enzimas que catalisam a hidrólise de ligações éster de óleos e gorduras com diferentes especificidades e podem, em meio orgânico, catalisar uma variedade de reações, como esterificação, interesterificação, transesterificação e aminólise. As lipases têm sido amplamente empregadas na produção de fármacos, emulsificantes, alimentos, perfumaria, diagnósticos médicos, compostos opticamente ativos, polímeros, aromas e fragrâncias, modificações de lipídeos para a produção de biodiesel e lipídeos estruturados e no pré-tratamento de efluentes com elevado teor de lipídeos gerados pelas indústrias de alimentos. [11][12][13][14][15] Essas enzimas encontram-se largamente distribuídas na natureza em tecidos animais e vegetais e biomassa microbiana. [11][12][13][14][15][16] Dentre elas, as lipases microbianas são as mais utilizadas industrialmente. 6,12,13 Por outro lado, as lipases vegetais apresentam algumas vantagens em relação às lipases microbianas e animais como ampla disponibilidade, baixo custo e elevada especificidade. 14-16 As principais fontes de lipases vegetais são o látex de frutos do gênero Carica como baba...

show abstract

Lipase activity in biphasic media: Why interfacial area is a significant parameter?

Cited by 25 publications

References 29 publications

Pickering Interfacial Catalysis for Biphasic Systems: From Emulsion Design to Green Reactions

Pickering Interfacial Catalysis for Biphasic Systems: From Emulsion Design to Green Reactions

Amphiphilic Carbonaceous Microsphere‐Stabilized Oil‐in‐Water Pickering Emulsions and Their Applications in Enzyme Catalysis

Produção de concentrados de ácidos graxos por hidrólise de óleos vegetais mediada por lipase vegetal

Contact Info

Product

Resources

About