Linseed Oil Attenuates Liver Inflammation, Fatty Acid Accumulation, and Lipid Distribution in Periportal and Perivenous Hepatocytes Induced by a High-Carbohydrate Diet in Mice

Godoy, Guilherme; Antunes, Marina Masetto; Fernandes, Ingrid de Lima; Manin, Luciana Pelissari; Zappielo, Caroline D.; Masi, Laureane Nunes; Perles, Juliana Vanessa Colombo Martins; Visentainer, Jesuí Vergílio; Curi, Rui; Bazotte, Roberto Barbosa

doi:10.1089/jmf.2022.0031

Cited by 2 publications

(8 citation statements)

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“…The L100 diet had more significant effects than the L10 diet. In agreement with this observation, we previously reported that the L100 diet, but not the L10 diet, prevented the elevation of blood triacylglycerol levels promoted by HCD (5).…”

Section: Discussionsupporting

confidence: 87%

“…These alterations were prevented or attenuated if the lipid source of the HCD (lard and soybean oil) was replaced by 10% (L10 group) or 100% (L100 group) linseed oil. Similary, our previous study using the same experimental model (5) demonstrated that the percentage of lipid-occupied area was higher in PVH, while the L10 diet and L100 diet prevented or decreased these alterations. Liver fibrosis induced by the HCD was not homogeneous.…”

Section: Discussionsupporting

confidence: 86%

“…Based on previous studies ( 5 , 7 , 11 , 14 ), we determined that 56 days of the diet is sufficient to induce NAFLD. This period also allows for the evaluation of the preventive or attenuating effects of L10 or L100 ( 5 ).…”

Section: Methodsmentioning

confidence: 99%

“…The progression of NAFLD includes reversible lipid accumulation, non-reversible non-alcoholic steatohepatitis (NASH), which is associated with inflammation and discrete fibrosis process, and aggressive non-reversible cirrhosis, which is characterized by inflammation, consolidation of the fibrotic process, and liver failure ( 4 ). The investigation of NAFLD progression includes morphological evaluation, histopathological analysis, and quantification of biomarkers for inflammation and the fibrotic process ( 5 ).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, we demonstrated that increased inflammation, higher FA deposition, and increased lipid-occupied area in periportal hepatocytes (PPH) and perivenous hepatocytes (PVH) induced by HCD were prevented or attenuated by linseed oil as 10% (L10) or 100% (L100) of the lipid source ( 5 ). In this study, we expand upon these findings by assessing the impact of L10 and L100 on liver injury induced by HCD in PVH and PPH of the medium lobe (ML) and left lobe (LL).…”

Section: Introductionmentioning

confidence: 99%

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Linseed oil attenuates fatty liver disease in mice fed a high-carbohydrate diet

Godoy,

Bernardo,

Casagrande

et al. 2023

Braz J Med Biol Res

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The impact of linseed oil as a lipid source on liver disease induced by a high-carbohydrate diet (HCD) was evaluated. Adult male Swiss mice received an HCD containing carbohydrates (72.1%), proteins (14.2%), and lipids (4.0%). The Control HCD group (HCD-C) received an HCD containing lard (3.6%) and soybean oil (0.4%) as lipid sources. The L10 and L100 groups received an HCD with 10 and 100% linseed oil as lipid sources, respectively. A group of mice were euthanized before receiving the diets (day 0) and the remaining groups after 56 days of receiving the diets (HCD-C, L10, and L-100 groups). Morphological and histopathological analyses, as well as collagen deposition were evaluated. Perivenous hepatocytes (PVH) of the HCD-C group were larger (Po0.05) than periportal hepatocytes (PPH) in the median lobe (ML) and left lobe (LL). There was a greater (Po0.05) deposition of type I collagen in PPH (vs PVH) and in the ML (vs LL). The ML exhibited a higher proportion of apoptotic bodies, inflammatory infiltrate, and hepatocellular ballooning. All these alterations (hepatocyte size, deposition of type I collagen, apoptotic bodies, inflammatory infiltrate, and hepatocellular ballooning) induced by HCD were prevented or attenuated in L10 and L100 groups. Another indicator of the beneficial effects of linseed oil was the lower (Po0.05) number of binucleated hepatocytes (HCD-C vs L10 or L100 group). In general, the L100 group had greater effects than the L10 group. In conclusion, linseed oil impedes or reduces the liver injury progression induced by an HCD.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 86%