Linoleic acid, a plant fatty acid, controls membrane biofouling via inhibition of biofilm formation

Kim, Han Shin; Ham, So Young; Jang, Yongsun; Sun, Peng; Park, Jeong Hoon; Lee, Jeung Hoon; Park, Hee Deung

doi:10.1016/j.fuel.2019.05.064

Cited by 21 publications

(11 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Various fatty acids possess ability to inhibit biofilm development by affecting the adhering surface and cell membrane fluidity [28]. For example, linoleic acid inhibits P. aeruginosa biofilms by increasing the membrane permeability of the bacteria [26]. Given the close relationships observed among persister cells, biofilms, and virulence factors, as well as our observations that undecanoic acid, lauric acid, and N-tridecanoic acid exhibited antipersister and antibiofilm activities, medium-chain saturated fatty acids may play important roles by connecting persister cell repression, biofilm inhibition, and potentially virulence regulation.…”

Section: Fatty Acids Repress Ehec Biofilm Formationmentioning

confidence: 99%

Undecanoic Acid, Lauric Acid, and N-Tridecanoic Acid Inhibit Escherichia coli Persistence and Biofilm Formation

Jin

Zhou

Richey

et al. 2021

J. Microbiol. Biotechnol.

View full text Add to dashboard Cite

Persister cell formation and biofilms of pathogens are extensively involved in the development of chronic infectious diseases. Eradicating persister cells is challenging, owing to their tolerance to conventional antibiotics, which cannot kill cells in a metabolically dormant state. A high frequency of persisters in biofilms makes inactivating biofilm cells more difficult, because the biofilm matrix inhibits antibiotic penetration. Fatty acids may be promising candidates as antipersister or antibiofilm agents, because some fatty acids exhibit antimicrobial effects. We previously reported that fatty acid ethyl esters effectively inhibit Escherichia coli persister formation by regulating an antitoxin. In this study, we screened a fatty acid library consisting of 65 different fatty acid molecules for altered persister formation. We found that undecanoic acid, lauric acid, and N-tridecanoic acid inhibited E. coli BW25113 persister cell formation by 25-, 58-, and 44-fold, respectively. Similarly, these fatty acids repressed persisters of enterohemorrhagic E. coli EDL933. These fatty acids were all medium-chain saturated forms. Furthermore, the fatty acids repressed Enterohemorrhagic E. coli (EHEC) biofilm formation (for example, by 8-fold for lauric acid) without having antimicrobial activity. This study demonstrates that medium-chain saturated fatty acids can serve as antipersister and antibiofilm agents that may be applied to treat bacterial infections.

show abstract

Section: Fatty Acids Repress Ehec Biofilm Formationmentioning

confidence: 99%

Undecanoic Acid, Lauric Acid, and N-Tridecanoic Acid Inhibit Escherichia coli Persistence and Biofilm Formation

Jin

Zhou

Richey

et al. 2021

J. Microbiol. Biotechnol.

View full text Add to dashboard Cite

show abstract

“… P. aeruginosa PA14 is a representative bacterium that forms biofilms via QS systems ( 47 ). The PA14 strain was incubated in AB medium (300 mM NaCl, 50 mM MgSO 4 , 0.2% casamino acids, 10 mM potassium phosphate, 1 mM l -arginine, and 1% glucose, pH 7.5) at 37°C for 12 h under constant shaking (200 rpm) ( 48 ).…”

Section: Methodsmentioning

confidence: 99%

Combined Treatment of 6-Gingerol Analog and Tobramycin for Inhibiting Pseudomonas aeruginosa Infections

Ham

Kim

et al. 2021

Microbiol Spectr

Self Cite

View full text Add to dashboard Cite

show abstract

“…This result shows that P. aeruginosa growth was not affected by up to 100 µM LA (Figure 1b). Although we demonstrated that LA inhibited biofilm formation by P. aeruginosa in a previous study (H.-S. Kim et al, 2019), it was now needed to include a positive control (cis-2-DA) and different LA concentrations (10 and 100 μM) for investigating biofilm inhibitory mechanism by LA. The effect of LA and cis-2-DA on P. aeruginosa biofilm formation was evaluated using a static biofilm assay in a 96-well plate.…”

Section: Effect Of La On Growth and Biofilm Formationmentioning

confidence: 95%

Linoleic acid inhibits Pseudomonas aeruginosa biofilm formation by activating diffusible signal factor‐mediated quorum sensing

Kim

Cha

Ham

et al. 2020

Biotech & Bioengineering

View full text Add to dashboard Cite

Bacterial biofilm formation causes serious problems in various fields of medical, clinical, and industrial settings. Antibiotics and biocide treatments are typical methods used to remove bacterial biofilms, but biofilms are difficult to remove effectively from surfaces due to their increased resistance. An alternative approach to treatment with antimicrobial agents is using biofilm inhibitors that regulate biofilm development without inhibiting bacterial growth. In the present study, we found that linoleic acid (LA), a plant unsaturated fatty acid, inhibits biofilm formation under static and continuous conditions without inhibiting the growth of Pseudomonas aeruginosa. LA also influenced the bacterial motility, extracellular polymeric substance production, and biofilm dispersion by decreasing the intracellular cyclic diguanylate concentration through increased phosphodiesterase activity. Furthermore, quantitative gene expression analysis demonstrated that LA induced the expression of genes associated with diffusible signaling factor-mediated quorum sensing that can inhibit or induce the dispersion of P. aeruginosa biofilms. These results suggest that LA is functionally and structurally similar to a P. aeruginosa diffusible signaling factor (cis-2-decenoic acid) and, in turn, act as an agonist molecule in biofilm dispersion.

show abstract

Linoleic acid, a plant fatty acid, controls membrane biofouling via inhibition of biofilm formation

Cited by 21 publications

References 34 publications

Undecanoic Acid, Lauric Acid, and N-Tridecanoic Acid Inhibit Escherichia coli Persistence and Biofilm Formation

Undecanoic Acid, Lauric Acid, and N-Tridecanoic Acid Inhibit Escherichia coli Persistence and Biofilm Formation

Combined Treatment of 6-Gingerol Analog and Tobramycin for Inhibiting Pseudomonas aeruginosa Infections

Linoleic acid inhibits Pseudomonas aeruginosa biofilm formation by activating diffusible signal factor‐mediated quorum sensing

Contact Info

Product

Resources

About