Linking performance to microbiology in biofilters treating dimethyl sulphide in the presence and absence of methanol

Hayes, Alexander C.; Zhang, Yuefeng; Liss, Steven N.; Allen, D. Grant

doi:10.1007/s00253-009-2272-5

Cited by 25 publications

(15 citation statements)

References 38 publications

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“…Comparative analysis based on 16S rDNA sequences revealed that the bacterial community in the bagasse pile was mostly related to the bacteria in the soil and compost (EU676446, GQ360076), 23) especially from high-temperature sites (AM749771, GU113028), 24) A substantial number of the sequences were also related to the microbial assemblage in geothermal ecosystems e.g., hot spring, 11) and the thermophilic cyanobacterial mat (EF032761) as well as in waste water sludge (FJ536903). 25) Although the analysis might have been biased due to the availability of sequences from different environments in the existing databases, this suggests significant similarities and differences between the microbial groups in bagasse piles and other ecosystems.…”

Section: Prokaryotic Microbial Diversitymentioning

confidence: 99%

Culture-Independent Phylogenetic Analysis of the Microbial Community in Industrial Sugarcane Bagasse Feedstock Piles

Rattanachomsri

Kanokratana

Eurwilaichitr

et al. 2011

Bioscience, Biotechnology, and Biochemistry

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Section: Prokaryotic Microbial Diversitymentioning

confidence: 99%

Culture-Independent Phylogenetic Analysis of the Microbial Community in Industrial Sugarcane Bagasse Feedstock Piles

Rattanachomsri

Kanokratana

Eurwilaichitr

et al. 2011

Bioscience, Biotechnology, and Biochemistry

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“…This was evident in our previous study in which the biofilter treating DMS alone was characterized by chronically low DMS removal rates and removal efficiencies in a community where Hyphomicrobium spp. accounted for 10% of the bacterial population on a 16S rRNA gene basis (16). Second, these results show that in systems where pH may be difficult to control, such as traditional biofiltration systems, methanol addition can lead to higher DMS removal rates and removal efficiencies under conditions that are not conducive to high growth rates of Hyphomicrobium spp.…”

Section: Discussionmentioning

confidence: 77%

“…An enrichment culture was created by inoculating mineral medium with biomass collected from a biofilter cotreating DMS and methanol and by feeding the culture DMS as the sole organic carbon source. A Bacteriawide Bayesian phylogeny revealed that the enrichment culture was composed of a wide variety of bacteria, and a comparison of this clone library with one constructed from a biofilter treating DMS alone revealed three groups of bacteria (Hyphomicrobium, Thiobacillus, and Chitinophaga) that appear to be tightly correlated to DMS degradation in these systems (16). Minimum evolution phylogenies of the Hyphomicrobium and Thiobacillus genera and the Chitinophaga family reveal that while there is genetic diversity in the 16S rRNA gene sequences within each of these groups, the identified clones from Thiobacillus spp.…”

Section: Resultsmentioning

confidence: 99%

“…It was demonstrated that the DMS degradation rate in biofilters where the pH of the biofilter bed was allowed to acidify naturally over time to pH 5 before the pH was neutralized back to pH 7 could be increased by up to 11-fold with methanol cotreatment (47). The increase in the DMS removal rates was shown to be the result of an order of magnitude increase in the concentration of Hyphomicrobium spp., which were also capable of growth on methanol (16). Methanol addition also resulted in a decrease in the full conversion of DMS to sulfate (increase in S 0 ) and nitrification in biofilters cotreating DMS and methanol compared to the biofilter treating DMS alone, resulting in a decrease in the rate of acidification in these biofilters (48).…”

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confidence: 99%

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Growth Kinetics of Hyphomicrobium and Thiobacillus spp. in Mixed Cultures Degrading Dimethyl Sulfide and Methanol

Hayes

Liss

Allen

2010

Appl Environ Microbiol

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The growth kinetics of Hyphomicrobium spp. and Thiobacillus spp. on dimethyl sulfide (DMS) and methanol (in the case of Hyphomicrobium spp.) in an enrichment culture created from a biofilter cotreating DMS and methanol were studied. Specific growth rates of 0.099 h ؊1 and 0.11 h ؊1 were determined for Hyphomicrobium spp. and Thiobacillus spp., respectively, growing on DMS at pH 7. These specific growth rates are double the highest maximum specific growth rate for bacterial growth on DMS reported to date in the literature. When the pH of the medium was decreased from pH 7 to pH 5, the specific growth rate of Hyphomicrobium spp. decreased by 85%, with a near 100-fold decline in the yield of Hyphomicrobium 16S rRNA gene copies in the mixed culture. Through the same pH shift, the specific growth rate and 16S rRNA gene yield of Thiobacillus spp. remained similar. When methanol was used as a substrate, the specific growth rate of Hyphomicrobium spp. declined much less over the same pH range (up to 30%) while the yield of 16S rRNA gene copies declined by only 50%. Switching from an NH 4 ؉ -N-based source to a NO 3 ؊ -N-based source resulted in the same trends for the specific growth rate of these microorganisms with respect to pH. This suggests that pH has far more impact on the growth kinetics of these microorganisms than the nitrogen source. The results of these mixed-culture batch experiments indicate that the increased DMS removal rates observed in previous studies of biofilters cotreating DMS and methanol are due to the proliferation of DMS-degrading Hyphomicrobium spp. on methanol at pH levels not conducive to high growth rates on DMS alone.Dimethyl sulfide (DMS) is a reduced sulfur compound that is emitted from both natural and anthropogenic sources. Natural DMS emissions are largely the result of the cleavage of dimethylsulfoniopropionate (9), the breakdown of the sulfurcontaining amino acids methionine and cysteine (9, 11), and the degradation of methoxylated aromatic compounds (3, 9). Anthropogenic DMS emissions tend to be the result of hightemperature industrial processes and are problematic due to the foul smell of DMS and its low odor threshold (34). Industries that are sources of anthropogenic DMS emissions include wastewater treatment (14), aerobic composting (40), animal rendering (23), and kraft pulping (35).In the environment, microbial degradation can be a significant sink for DMS. In seawater, approximately 90% of the DMS produced is removed biologically before it reaches the atmosphere (21). Removal of DMS in the environment can be carried out by a variety of pathways. Aerobic bacteria, such as Hyphomicrobium spp. The prevalence of bacteria in the environment capable of growth on DMS has created interest in developing low-cost biotechnological methods to remove DMS from industrial waste gas streams. One possible technology is biofiltration which involves passing waste air through a packed bed of microorganisms. Removal of DMS in these systems, however, has proved to be difficult. This is believed t...

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“…The high growth rate of Hyphomicrobium species might have favoured their growth in laboratory conditions. Hayes et al (2010) estimated the growth rate of Hyphomicrobium spp. growing on DMS to be 0.099 h -1 .…”

Section: Discussionmentioning

confidence: 99%

Culture-dependent and culture-independent methods reveal diverse methylotrophic communities in terrestrial environments

Eyice

Schäfer

2015

Arch Microbiol

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Original citation:Eyice, Özge and Schäfer, Hendrik. (2015) Culture-dependent and culture-independent methods reveal diverse methylotrophic communities in terrestrial environments. Archives of Microbiology. Permanent WRAP url:http://wrap.warwick.ac.uk/73935 Copyright and reuse:The Warwick Research Archive Portal (WRAP) makes this work by researchers of the University of Warwick available open access under the following conditions. Copyright © and all moral rights to the version of the paper presented here belong to the individual author(s) and/or other copyright owners. To the extent reasonable and practicable the material made available in WRAP has been checked for eligibility before being made available.Copies of full items can be used for personal research or study, educational, or not-for profit purposes without prior permission or charge. Provided that the authors, title and full bibliographic details are credited, a hyperlink and/or URL is given for the original metadata page and the content is not changed in any way. Publisher's statement:The final publication is available at Springer via http://dx.doi.org/10.1007/s00203-015-1160-x A note on versions: The version presented here may differ from the published version or, version of record, if you wish to cite this item you are advised to consult the publisher's version. Please see the 'permanent WRAP url' above for details on accessing the published version and note that access may require a subscription. They are suggested to affect atmospheric chemistry and global climate. Methylotrophic microorganisms are considered as a significant sink for these compounds, therefore we analysed the diversity of terrestrial bacteria that utilise methanol, DMS and DMSO as carbon and energy source using culture-dependent and -independent methods. The effect of habitat type on the methylotrophic community structure was also investigated in rhizosphere and bulk soil. While thirteen strains affiliated to the genera

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Linking performance to microbiology in biofilters treating dimethyl sulphide in the presence and absence of methanol

Cited by 25 publications

References 38 publications

Culture-Independent Phylogenetic Analysis of the Microbial Community in Industrial Sugarcane Bagasse Feedstock Piles

Culture-Independent Phylogenetic Analysis of the Microbial Community in Industrial Sugarcane Bagasse Feedstock Piles

Growth Kinetics of Hyphomicrobium and Thiobacillus spp. in Mixed Cultures Degrading Dimethyl Sulfide and Methanol

Culture-dependent and culture-independent methods reveal diverse methylotrophic communities in terrestrial environments

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