Linker-peptide-mediated one-step purification and immobilization of α-L-rhamnosidase from Bacteroides thetaiotaomicron for direct biotransformation from epimedin C to icariin

Lu, Changning; Zhang, Kang; Guo, Biying; Li, Qi; Wang, Zhenzhong; Xiao, Wei; Zhao, Linguo

doi:10.1016/j.enzmictec.2022.110131

Cited by 2 publications

(1 citation statement)

References 42 publications

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“…ICRN (C 33 H 40 O 15 , M w = 676.67) is a type of prenylated flavonoid, showing extensive bioactivities such as antioxidant [ 44 , 45 , 46 , 47 ], neuroprotective [ 48 , 49 , 50 , 51 , 52 ], and antitumor [ 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 ] behavior as well as anti-inflammatory responses [ 62 , 63 , 64 , 65 , 66 , 67 , 68 ], and can be used to treat erectile dysfunction [ 69 , 70 , 71 , 72 ]. It appears it can improve the function of organs including bones and the heart [ 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 ].…”

Section: Physicochemical Propertiesmentioning

confidence: 99%

Icariin: A Promising Natural Product in Biomedicine and Tissue Engineering

Seyedi¹,

Amiri

Mohammadzadeh

et al. 2023

JFB

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Among scaffolds used in tissue engineering, natural biomaterials such as plant-based materials show a crucial role in cellular function due to their biocompatibility and chemical indicators. Because of environmentally friendly behavior and safety, green methods are so important in designing scaffolds. A key bioactive flavonoid of the Epimedium plant, Icariin (ICRN), has a broad range of applications in improving scaffolds as a constant and non-immunogenic material, and in stimulating the cell growth, differentiation of chondrocytes as well as differentiation of embryonic stem cells towards cardiomyocytes. Moreover, fusion of ICRN into the hydrogel scaffolds or chemical crosslinking can enhance the secretion of the collagen matrix and proteoglycan in bone and cartilage tissue engineering. To scrutinize, in various types of cancer cells, ICRN plays a decisive role through increasing cytochrome c secretion, Bax/Bcl2 ratio, poly (ADP-ribose) polymerase as well as caspase stimulations. Surprisingly, ICRN can induce apoptosis, reduce viability and inhibit proliferation of cancer cells, and repress tumorigenesis as well as metastasis. Moreover, cancer cells no longer grow by halting the cell cycle at two checkpoints, G0/G1 and G2/M, through the inhibition of NF-κB by ICRN. Besides, improving nephrotoxicity occurring due to cisplatin and inhibiting multidrug resistance are the other applications of this biomaterial.

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Section: Physicochemical Propertiesmentioning

confidence: 99%

Icariin: A Promising Natural Product in Biomedicine and Tissue Engineering

Seyedi¹,

Amiri

Mohammadzadeh

et al. 2023

JFB

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Efficient preparation of icariin from epimedin C by recyclable biphasic enzymatic hydrolysis

Chen,

Zhang,

Zhang

et al. 2024

Natural Product Research

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Icariin is the most bioactive ingredient of Epimedium L. and a quality marker of Herba Epimedii. Conventional methods for production of Icariin exhibit very low efficiency with poor yield and serious pollution. This study aimed to establish an efficient and recyclable biphasic enzymatic hydrolysis system for the efficient conversion of epimedin C to icariin. The biphasic system was constructed with butyl acetate and phosphate buffer (pH 4.5) (3:1, V/V) containing α-L-rhamnosidase/epimedin C (2U/1 mg) at 50°C for 12 h. Consequently, 98.21% of epimedin C was hydrolysed to icariin, and 95.62% of the product was transferred into the organic phase. Moreover, the conversion ratio reached 75.28% even after the enzyme solution was used for four cycles. Additionally, this novel strategy was also used for the conversion of Epimedium brevicornu Maxim. extracts. The biphasic system is clean and efficient approach for the preparation of icariin, and provides a scientific basis for industrial applications.

show abstract

Linker-peptide-mediated one-step purification and immobilization of α-L-rhamnosidase from Bacteroides thetaiotaomicron for direct biotransformation from epimedin C to icariin

Cited by 2 publications

References 42 publications

Icariin: A Promising Natural Product in Biomedicine and Tissue Engineering

Icariin: A Promising Natural Product in Biomedicine and Tissue Engineering

Efficient preparation of icariin from epimedin C by recyclable biphasic enzymatic hydrolysis

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