1988
DOI: 10.1007/bf00346157
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Linked genetic markers of the rabbit ? light chain are not linked to the Tcr ? chain genes

Abstract: In order to investigate linkage, we used serum allotypes of the two rabbit C kappa isotypes and restriction fragment length polymorphisms (RFLPs) of the genes for V kappa, C kappa, and T-cell receptor C beta. The inheritance of these genetic markers was studied through backcross and F2 matings. Southern analysis and hybridization of genomic DNA with a C kappa probe detected a 5 kb Pst I fragment linked to expression of the K2bas1 allotype and the presence of the kappa 1bbas gene and a 6.6 kb Pst I fragment lin… Show more

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Cited by 2 publications
(4 citation statements)
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“…For example, the J, probe which identifies a 10-kb Bam HI fragment in the DNA from b9.1 rabbits, identifies a 17-kb fragment in the DNA from b9.2 rabbits. The presence of the 17-kb fragment is linked to the presence of K2basl allotype in b9.2 and the 10-kb fragment to its absence in b9.1.We confirmed and extended a previous genetic study in which we showed linkage of allotype to a Pst I RFLP of C, and to an Eco RI RFLP of V, [20]. Complete linkage of the presence of the 17-kb fragment to the production of the …”
Section: Restriction Maps and Rflpsupporting
confidence: 89%
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“…For example, the J, probe which identifies a 10-kb Bam HI fragment in the DNA from b9.1 rabbits, identifies a 17-kb fragment in the DNA from b9.2 rabbits. The presence of the 17-kb fragment is linked to the presence of K2basl allotype in b9.2 and the 10-kb fragment to its absence in b9.1.We confirmed and extended a previous genetic study in which we showed linkage of allotype to a Pst I RFLP of C, and to an Eco RI RFLP of V, [20]. Complete linkage of the presence of the 17-kb fragment to the production of the …”
Section: Restriction Maps and Rflpsupporting
confidence: 89%
“…Mapping was performed on the DNA from rabbits of the Canadian b9.1 strain originating from the University of Toronto and having the Klb9 and =bas2 x allotypes [20]. In addition, some comparisons were made with the DNA from the Swiss b9.2 and Basilea strains originating from the Basel Institute for Immunology [3, 51; these differ from b9.1 by RFLP associated with the K2 gene [20] and by expressing the K2basl allotype [2, 201. isolated from tissues in 100 p1 of 0.5% InCertTM agarose SSPE [22], 1% SDS, 10 X Denhardt's solution and (FMC Corp., Rockland, ME) using LKB molds (LKB-0.5 mg/ml salmon sperm DNA.…”
Section: Rabbitsmentioning
confidence: 99%
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