Linked bridge hybridizing-induced split G-quadruplex DNA machine and its application to uracil-DNA glycosylase detection

Distance-Based Biosensor for Ultrasensitive Detection of Uracil-DNA Glycosylase Using Membrane Filtration of DNA Hydrogel

“…To compare the analysis performance between the proposed method and other reported methods, − ,− a study shown in Table S2 is carried out on an operational strategy, required materials, linear range, and MDC. In comparison with the reported colorimetric assay (8 mU/mL), as low as 0.02 mU/mL of UDG can be detected by visualizing the distance of the red indicator solution in the capillary, and the sensitivity has enhanced by 400-fold.…”

“…However, this method suffers from some drawbacks, such as radioactive hazard, laborious, and low sensitivity. Alternatively, some nonradioactive methods, including SERS, colorimetric methods, electrochemistry, , and fluorescent methods, have been proposed, but they suffer from poor sensitivity and complicated operation. In order to improve sensitivity, many efforts have been devoted to developing amplification strategies. − Nevertheless, these methods require either fluorescently labeled probes, enzyme-assisted amplification reaction, or complicated probe design.…”

Exploring the fluorescence enhancement of the split G-quadruplex towards DNA-templated AgNCs and their application in omethoate detection

“…In the field of analysis, the three-element system (spit G4/target/spit G4) can improve the detection platform's performance owing to its high recognition and self-examination ability. [14][15][16][17][18][19][20] Due to the important roles of split G4 in the analysis field, many researchers have researched the most effective split mode. Wang's group first found that the split mode of 4 : 8 performs best in the presence of protoporphyrin IX (PPIX) among six different split modes.…”

A Methodology for Ultrasensitive Detection of Sequence-Specific DNA or Uracil-DNA Glycosylase Activity

“…Recently, the base excision repair (BER) has been intensively researched . As one of the BER enzymes existing in most organisms, UDG can characteristically recognize and transform the N -glycosidic linkage between the uracil and deoxyribose to the apurinic/apyrimidinic site (AP). , However, abnormal UDG activity can hinder the procedure of the uracil-included BER process, causing various lesions such as chemotherapy resistance, lymphoma, cancer, neurodegenerative diseases, bloom syndrome, and human immunodeficiency . Moreover, abnormalities in UDG activity generally occur far before other signs of malignancy and could be used as a potential biomarker and therapeutic target of early-stage diseases.…”

“…Moreover, abnormalities in UDG activity generally occur far before other signs of malignancy and could be used as a potential biomarker and therapeutic target of early-stage diseases. Traditional methods, such as gel electrophoresis, mass spectroscopy, electrochemistry, chemiluminescence, colorimetry, and radioisotopic labeling, have been applied to detect the activity of UDG. ,− However, these methods have some shortcomings such as time-consuming, poor sensitivity, and complicated operation. Therefore, establishing a method, which can accurately, more sensitively, and effectively detect the UDG activity is significant for both clinical diagnostic and biomedical studies.…”