Linkage relationships of mutations endowing Streptococcus pyogenes with resistance to antibiotics that affect the ribosome

Malke, Horst

doi:10.1007/bf00270087

Cited by 22 publications

(6 citation statements)

References 25 publications

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“…The present study of antibiotic resistance markers of chromosomal origin substantiates linkage data previously reported by Malke (14), and, further, orders four new antibiotic resistance markers within two linkage groups. Additional data establish the presence of a third linkage group including resistance to rifampin and streptolydigan (stl).…”

supporting

confidence: 91%

“…Linkage of ery and spr was not surprising, since they are both macrolide antibiotics and have similar modes of action (17). Presumably lincomycin resistance (lin) is located within this linkage group, since Malke (14) reported linkage of spc-ery-lin, in that order. Unfortunately, Linr mutants were not isolated in this study and could not be ordered within the linkage group spc-ery-spr.…”

mentioning

confidence: 98%

“…Studies on the genetics of antibiotic resistance in Streptococcuspyogenes were started by Leonard et al (12), who described transduction of streptomycin resistance. Subsequently, Malke (13,14) refined the transduction technique and described two groups of markers that were cotransduced into sensitive recipients. In one linkage group, resistance to streptomycin (str) was cotransduced at high frequency with resistance to kanamycin (kan), and in another group resistance to spectinomycin (spc), erythromycin (ery), and lincomycin (lin) were found to be tightly linked in the order described.…”

mentioning

confidence: 99%

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Genetic analysis of antibiotic resistance in Streptococcus pyogenes

Stuart¹,

Ferretti²

1978

J Bacteriol

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The genetics of antibiotic resistance in mutant strains of Streptococcus pyogenes was studied. Utilizing a type 6 strain (9440) primarily resistant to streptomycin (Strr), classes of mutant strains were isolated that were resistant to one of the following antibiotics: rifampin (RiP), erythromycin (Eryr), thiostrepton (Tstr), spiramycin (Spr), fusidic acid (Fusr), gramicidin (Grcr), ethidium bromide (Ebrr), kanamycin (Kanr), neomycin (Neor), oleandomycin (Oler), gentamicin (Genr), and novobiocin (Novr). Transduction experiments separated antibiotic resistance markers into two distinct groups: transducible markers, including Fusr, Bacr, Ksg, SpCr, EYr, Sprr, Rift, Stlr, and Tstr (Bacr, Ksgr, Spcr, and Stlr refer to resistance to bacitracin, kasugamycin, spectinomycin, and streptolydigan, respectively), and nontransducible markers, including Grcr, Ebrr, Kanr, Neor, Oler, Genr, and Novr. By means of two-and three-point crosses, transducible markers (excluding tst) were located in three separate linkage groups. spr was found to be linked with ery and spc in the order spc-ery-spr, whereas in a separate linkage on August 1, 2020 by guest

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supporting

confidence: 91%

mentioning

confidence: 98%

mentioning

confidence: 99%

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Genetic analysis of antibiotic resistance in Streptococcus pyogenes

Stuart¹,

Ferretti²

1978

J Bacteriol

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“…Previous data (MALKE 1970b) have shown that eryA500 is more closely linked to eryAlOO than eryA200 t o eryA2000, and this result is consistent with the order of markers suggested above. Furthermore, the results of three-point crosses (MALKE 1972) substantiate that the eryA and linA mutations studied here were not intermingled but occurred a t distinct loci.…”

Section: Uw-60supporting

confidence: 75%

Transductional analysis of resistance to lincomycin and erythromycin in Streptococcus pyogenes

Malke¹

1972

J of Basic Microbiology

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From the group A streptococcal strain 56188, mutants were selected for resistance t o lincomycin. Among these, two distinguishable classes occurred: whereas mutations assigned to the ZinA locus did not change the susceptibility to erythromycin and were transducible by phage A25, class B mutations conferred erythromycin cross-resistance and failed to be transducible under the conditions employed. Corresponding findings have previously been made concerning the properties of mutants selected for resistance to erythromycin.By two-point transduction crosses, the eryA and ZinA loci were placed in the same linkage group, as defined by cotransfer with phage A25. A common feature of mutants carrying both an eryA and a ZinA mutation was the slower growth compared to that of the parental strains bearing either mutation alone. This interaction among eryA and ZinA mutations proved to be strongest in certain combinations which were phenotypically unrecognizable because the resultant strains failed to develop colonies under the conditions used. On the other hand, the mutations of the established double mutants did not interact with respect to their effect on the resistance characteristics of the strains, which were the simple addition of the effects of the single mutations. Both the linkage relationships and the phenotypic properties of the eryA and ZinA mutations suggested that they might occur in genes coding for ribosomal proteins.Mutations carried out by the B classes of the erythromycin-resistant and lincomycin-resistant strains appeared to be located outside the eryA ZinA linkage group in region@), the function of which is unknown.

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“…The methods of gene transfer most widely used in the group A streptococci have been transduction [1,2] and conjugation [3,4]. Although transduction has been useful in establishing several linkage groups of antibiotic resistance markers [5,6], and conjugation has been used in the transfer of selected genes [7], neither of these methods of gene transfer has been useful for work involving cloned DNA. Transformation is the method of choice for introduction of either native or genetically engi-neered DNA into microorganisms, although until recently this method of gene transfer had not been successfully demonstrated in the group A streptococci.…”

Section: Introductionmentioning

confidence: 99%

Electrotransformation of Streptococcus pyogenes with plasmid and linear DNA

Simon

Ferretti

1991

FEMS Microbiology Letters

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Electrotransformation was used to introduce both plasmid and linear DNA into Streptococcus pyogenes. The method was optimized using strain NZ131, for which transformation frequencies up to 10(7) per micrograms of plasmid DNA were obtained. A linear fragment of DNA, containing the streptokinase gene (ska) in which an internal fragment had been replaced with an erythromycin resistance gene (erm), was transformed into strain NZ131 with a frequency of 10(3) per micrograms DNA. The introduction of linear DNA into S. pyogenes by electrotransformation should be useful for future genetic analyses as well as targeted gene replacement.

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Linkage relationships of mutations endowing Streptococcus pyogenes with resistance to antibiotics that affect the ribosome

Cited by 22 publications

References 25 publications

Genetic analysis of antibiotic resistance in Streptococcus pyogenes

Genetic analysis of antibiotic resistance in Streptococcus pyogenes

Transductional analysis of resistance to lincomycin and erythromycin in Streptococcus pyogenes

Electrotransformation of Streptococcus pyogenes with plasmid and linear DNA

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