Linkage mapping of the Phg-1 and Co-1 4 genes for resistance to angular leaf spot and anthracnose in the common bean cultivar AND 277

Abstract

The Andean common bean AND 277 has the Co-14 and the Phg-1 alleles that confer resistance to 21 and eight races, respectively, of the anthracnose (ANT) and angular leaf spot (ALS) pathogens. Because of its broad resistance spectrum, Co-14 is one of the main genes used in ANT resistance breeding. Additionally, Phg-1 is used for resistance to ALS. In this study, we elucidate the inheritance of the resistance of AND 277 to both pathogens using F2 populations from the AND 277 × Rudá and AND 277 × Ouro Negro crosses and F2:3 families from the AND 277 × Ouro Negro cross. Rudá and Ouro Negro are susceptible to all of the above races of both pathogens. Co-segregation analysis revealed that a single dominant gene in AND 277 confers resistance to races 65, 73, and 2047 of the ANT and to race 63-23 of the ALS pathogens. Co-14 and Phg-1 are tightly linked (0.0 cM) on linkage group Pv01. Through synteny mapping between common bean and soybean we also identified two new molecular markers, CV542014450 and TGA1.1570, tagging the Co-14 and Phg-1 loci. These markers are linked at 0.7 and 1.3 cM, respectively, from the Co-14/Phg-1 locus in coupling phase. The analysis of allele segregation in the BAT 93/Jalo EEP558 and California Dark Red Kidney/Yolano recombinant populations revealed that CV542014450 and TGA1.1570 segregated in the expected 1:1 ratio. Due to the physical linkage in cis configuration, Co-14 and Phg-1 are inherited together and can be monitored indirectly with the CV542014450 and TGA1.1570 markers. These results illustrate the rapid discovery of new markers through synteny mapping. These markers will reduce the time and costs associated with the pyramiding of these two disease resistance genes.

Development and application of microsatellites in plant breeding

“…The co-segregation between these genes revealed the possibility of monitoring those genes indirectly through the use of g2303 molecular marker. Similar results were obtained by Gonçalves-Vidigal et al 2011). In this study, the findings might help plant breeding programs to reduce time and cost associated with pyramiding resistance genes to different pathogens.…”

Aggressiveness of Pseudocercospora griseola strains in common bean genotypes and implications for genetic improvement

“…Considering that the genes with greater effect present in these cultivars are not more effective for conferring resistance to the most frequent race of the pathogen, the pyramiding of such genes by means of marker-assisted selection (Miklas, 2006;Ragagnin et al, 2009;Singh and Schwartz, 2010;Gonçalves-Vidigal et al, 2011) for obtaining durable resistance to the pathogen is debatable.…”

“…However, no studies have been conducted that have investigated the main mechanism of genetic resistance in the P. griseola-common bean pathosystem. The main breeding strategies with a view toward durable resistance to P. griseola have considered only vertical resistance, particularly by the pyramiding of genes from backcrosses, with the help of marker-assisted selection (Miklas et al, 2006;Ragagnin et al, 2009;Singh and Schwartz, 2010;Gonçalves-Vidigal et al, 2011).…”

Alternative markers linked to the Phg-2 angular leaf spot resistance locus in common bean using the Phaseolus genes marker database

“…The PhaseolusGenes marker database has become an essential tool for cataloging bean marker diversity so that the breeding community can utilize marker information from multiple sources to facilitate MAS (Gonçalves-Vidigal et al, 2011Reinprecht et al, 2013;Oblessuc et al, 2013Oblessuc et al, , 2015Aranda et al, 2014;Keller et al, 2015;Sousa et al, 2015;Coimbra-Gonçalves et al, 2016;Perseguini et al, 2016). Because genomic resequencing of breeding parents is today a more routine practice with the lowered cost of sequencing, the PhaseolusGenes database should evolve from a marker database into a sequence database, which includes not only the current markers but also the increasingly large number (> 2,000) of genotypes that have been sequenced, including the 16 parental genotypes of the ABC projects (T. Miller and P. Gepts, unpubl.…”

“…Several common bean accessions had been identified from Andean and Mesoamerican gene pools and dominant, monogenic, loci conferring qualitative ALS resistance to specific pathogen races have been mapped in the bean genome. The Phg-1 locus, identified in AND277, is located on chromosome 1 at a distance of 1.3 cM from marker TGA1.1 (Queiroz et al, 2004;Gonçalves-Vidigal et al, 2011). Mesoamerican resistance locus, Phg-2, mapped qualitatively in Mexico 54 is located on chromosome 8 at a distance of 5.9 and 11.8 cM from markers SN02 and OPE04, respectively (Sartorato et al, 2000).…”