Linkage disequilibria between human leucocyte antigen‐B and closely linked microsatellites in the Croatian population

Grubić, Zorana; Štingl, Katarína; Žunec, Renata; Car, Helena; Čečuk-Jeličić, Esma; Brkljačić-Kerhin, Vesna

doi:10.1111/j.1399-0039.2006.00731.x

Cited by 11 publications

(23 citation statements)

References 44 publications

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“…1). They code for stress-induced cell surface molecules, which does not associate with 2 -microglobulin and does not appear to present peptides [2], due to the putative peptidebinding groove is too narrow to accommodate a ligand, suggesting that MICA is not an antigen presenting molecule [3].…”

Section: Introductionmentioning

confidence: 98%

MICA Gene and Relevance to Immune Responses in Organ Transplants and Inflammatory, Tumoral and Autoimmune Diseases

Muro¹,

López-Hernández²,

Jose³

et al. 2009

CIR

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MICA maps about 46 kb centromeric to the HLA-B locus and encodes a stress-induced cell-surface glycoprotein that is expressed in keratinocytes, fibroblasts, gastrointestinal epithelium and in other cell types. MICA is not associated with 2 -microglobulin and does not appear to present peptides. MICA displays a high degree of allelic polymorphism within the non-classical HLA gene loci. The functional significance of these polymorphisms is unknown although certain changes in the amino acid sequence of the protein influence the abnormal expression or the affinity in the interaction with NKG2D, its ligand on the surface of NK, T and T CD8 lymphocytes, affecting NK-cell activation and T-cell response modulation.NKG2D/MICA interaction has been shown to over-ride the inhibitory signal provided by Killer Inhibitory Receptors (KIR) and/or CD94/NKG2A/B molecules, which sense the presence of HLA-A, -B, -C and -E, respectively, on target cells. MICA-NKG2D complex is also a versatile ligand-receptor pair since NKG2D can act as a primary receptor or costimulatory molecule during immune responses. This dual function of NKG2D molecule depending on determined circumstances is still a matter of controversy.Indeed, many tumors express MICA on their surface and circulating soluble MICA also triggers the downregulation of NKG2D and impair lymphocyte cytotoxicity in tumoral escape, highlighting the therapeutic potential of anti-MICA antibodies to overcome immune suppression and effectuate tumor destruction. MICA is also implicated in organ transplants outcome, as MICA antigens elicit a very powerful antibody response in recipients of organ allograft. NKG2D ligand induction might also participate in the amplification loop that leads to tissue damage during aGVHD. MICA is also an important candidate gene for a number of clinically significant diseases including diabetes, rheumatoid arthritis and other autoimmune diseases. This review highlights the advances and limitations of the studies on MICA gene in several pathologies and critically discusses the most recent findings in this rapidly evolving field.

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Section: Introductionmentioning

confidence: 98%

MICA Gene and Relevance to Immune Responses in Organ Transplants and Inflammatory, Tumoral and Autoimmune Diseases

Muro¹,

López-Hernández²,

Jose³

et al. 2009

CIR

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“…The MIC genes include seven members (MICA-MICG); five of these are pseudogenes and gene fragments, whereas MICA and MICB are functional genes closely related to each other. They code for stress-induced cell surface molecules, do not associate with ␤ 2 -microglobulin, and do not appear to present peptides [2].…”

Section: Introductionmentioning

confidence: 99%

Allelic diversity of MICA gene and MICA/HLA-B haplotypic variation in a population of the Murcia region in southeastern Spain

et al. 2008

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“…Amplifications were performed in a volume of 15 ml containing 50 ng DNA, 2.5 mM dNTPs, MgCl 2 (concentration varied for each Msat), 5 U of Taq polymerase, 1.0 ml PCR buffer and 10 pmol of each primer. The cycling parameters were as previously reported [13]. After amplification, PCR products were run on a 6% polyacrylamide gel in an automated sequencer (ALFexpress, Amersham Pharmacia, Uppsala, Sweden).…”

Section: Hla Microsatellite Typingmentioning

confidence: 99%

Pilot Study of the Association between the HLA Region and Testicular Carcinoma among Croatian Patients

Gotovac¹,

Grubić²,

Kaštelan

et al. 2011

Urol Int

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Objectives: To analyze the distribution of HLA alleles and HLA microsatellite alleles in Croatian patients with testicular carcinoma, compare it with that of healthy controls and investigate whether the polymorphism within the HLA region could be associated with the development of testicular cancer. Methods: Genomic DNA was isolated from the peripheral blood of 24 patients with testicular germ cell tumors (TGCT). Patients and controls were typed for HLA class I and class II polymorphism by the PCR-SSO method. Nine HLA microsatellites were analyzed by PCR and electrophoresis in an automated sequencer. Results: No significant deviation in the distribution of frequencies at HLA class I alleles was observed between patients and controls. Among HLA class II alleles, a statistically significant increase in the frequency of the HLA-DPB1*1701 allele was found among patients. The frequency of the HLA-DRB1*07-DQA1*0201-DQB1*0202 haplotype was increased in patients in comparison to the controls. Analysis of HLA microsatellites showed an increased frequency of D6S291-3 allele (p_corr = 0.0455, OR = 3.05) among patients. Conclusions: The observed association of the disease and the DPB1*1701 allele as well as with the D6S291-3 allele suggests that this part of the HLA region might be involved in the pathogenesis of TGCT. Our data provide a basis for further studies about the correlation between the HLA region and testicular cancer.

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Linkage disequilibria between human leucocyte antigen‐B and closely linked microsatellites in the Croatian population

Cited by 11 publications

References 44 publications

MICA Gene and Relevance to Immune Responses in Organ Transplants and Inflammatory, Tumoral and Autoimmune Diseases

MICA Gene and Relevance to Immune Responses in Organ Transplants and Inflammatory, Tumoral and Autoimmune Diseases

Allelic diversity of MICA gene and MICA/HLA-B haplotypic variation in a population of the Murcia region in southeastern Spain

Pilot Study of the Association between the HLA Region and Testicular Carcinoma among Croatian Patients

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