Linhagens de <i>Aspergillus nidulans</i> como biossensores de efeitos genotóxicos e antigenotóxicos de agentes ambientais.

Abstract

Main aim is to províde a reliable approach to deal with the aspects related to induced genotoxicity, genomic protection and eukaryotic mitotic recombination in .4sperg///us n/du/a/7s. Genotoxicity was benzene fumes induced, and to test genomic protection, soybean (G/ycfne max) has been used on account of its putative anticarcinogenic properties. Main outcome is that transgenic soybean bears higher antigenotoxic properties than traditíonal soybean. This has been twofold confirmed through basic genetic approaches and molecular approaches, as well. In addltion, each experimental approach has been three times repeated. Additional important outcomes are: a-establishment of a reliable protocol to deal with the complexities of the epigenetic events, and b-likely use of present protocol and experimental setup to test other environmental agents of which antigenotoxic properties are either suspected, or need precise identification. Evidently, on account of present wide concern the transgenic foodstuffs, and many other biotechnological products, as well, are obvious candidates for similar approaches. Obtained results suggest dose relationships among eukaryotic DNA methylation, mitotic recombination, and other cells damaging events reputedly leading to carcinogenesis.