LingZhi oligopeptides amino acid sequence analysis and anticancer potency evaluation

Liu, Jie; Wang, Huailing; Luo, Qiang; Qiu, Shuqi; He, Zhendan; Liu, Zhigang; Yang, Liteng; Liu, Xiaoyu; Sun, Xizhuo

doi:10.1039/c9ra10400c

Cited by 4 publications

(4 citation statements)

References 27 publications

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“…Additionally, most triterpenoids were exclusively detected in the fruiting body, thereby establishing the fruiting body as a more promising candidate for the development of anti-tumor and anti-AIDS drugs ( 6 ). However, the chemical composition of Ganoderma is remarkably complex, and efforts are underway to broaden the research scope of its pharmacological constituents ( 28 , 29 ). We found that amino acids and their derivatives were the most abundant metabolites in each Ganoderma species, providing new insights for the development of Ganoderma products.…”

Section: Resultsmentioning

confidence: 99%

UPLC-ESI-MS/MS-based widely targeted metabolomics reveals differences in metabolite composition among four Ganoderma species

Wei-Ye,

Hong-Bo,

Rui-Heng

et al. 2024

Front. Nutr.

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The Chinese name “Lingzhi” refers to Ganoderma genus, which are increasingly used in the food and medical industries. Ganoderma species are often used interchangeably since the differences in their composition are not known. To find compositional metabolite differences among Ganoderma species, we conducted a widely targeted metabolomics analysis of four commonly used edible and medicinal Ganoderma species based on ultra performance liquid chromatography-electrospray ionization-tandem mass spectrometry. Through pairwise comparisons, we identified 575–764 significant differential metabolites among the species, most of which exhibited large fold differences. We screened and analyzed the composition and functionality of the advantageous metabolites in each species. Ganoderma lingzhi advantageous metabolites were mostly related to amino acids and derivatives, as well as terpenes, G. sinense to terpenes, and G. leucocontextum and G. tsugae to nucleotides and derivatives, alkaloids, and lipids. Network pharmacological analysis showed that SRC, GAPDH, TNF, and AKT1 were the key targets of high-degree advantage metabolites among the four Ganoderma species. Analysis of Gene Ontology and Kyoto Encyclopedia of Genes and Genomes demonstrated that the advantage metabolites in the four Ganoderma species may regulate and participate in signaling pathways associated with diverse cancers, Alzheimer’s disease, and diabetes. Our findings contribute to more targeted development of Ganoderma products in the food and medical industries.

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Section: Resultsmentioning

confidence: 99%

UPLC-ESI-MS/MS-based widely targeted metabolomics reveals differences in metabolite composition among four Ganoderma species

Wei-Ye,

Hong-Bo,

Rui-Heng

et al. 2024

Front. Nutr.

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“…Organic solvent extraction is usually used for proteins or peptides containing aromatic amino acids as well as polar side chains [32]. Liu et al prepared Ganoderma lucidum crude polypeptides using 2500 mL H 2 O/isopropanol (1:15 w/v) by maceration for 4 h [33]. Li et al used anhydrous ethanol to extract dried gecko powder to obtain gecko crude polypeptides [34].…”

Section: Solvent Extractionmentioning

confidence: 99%

“…In addition, in IEC the elution is accomplished by increasing the linear elution salt concentration or buffer pH, but in order to maintain the biological activity of the peptide, it is common to use a phosphate solution gradient elution [66]. Liu et al uploaded Ganoderma lucidum oligopeptide (LZO) LZO-A-3 onto a DEAE-52 cellulose anion exchange column after UF membrane separation and used (NH 4 ) 2 SO 4 solution for gradient elution to obtain a total of six fractions (A-LZO-3-1~A-LZO-3-6) [33]. Zhao et al obtained (Lycium barbarum protein) LBP-A-4 fractions from LBP by UF membrane separation as well as Phenyl Sepharose CL-4B hydrophobic chromatography, and then uploaded onto a DEAE-52 cellulose anion exchange column to obtain 10 fractions (LBP-A-4-1 to 10) after gradient elution with (NH 4 ) 2 SO 4 solution [67].…”

Section: Ion Exchange Chromatographymentioning

confidence: 99%

The Research Progress of Bioactive Peptides Derived from Traditional Natural Products in China

Zhang,

Liu,

Zhang

et al. 2023

Molecules

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Traditional natural products in China have a long history and a vast pharmacological repertoire that has garnered significant attention due to their safety and efficacy in disease prevention and treatment. Among the bioactive components of traditional natural products in China, bioactive peptides (BPs) are specific protein fragments that have beneficial effects on human health. Despite many of the traditional natural products in China ingredients being rich in protein, BPs have not received sufficient attention as a critical factor influencing overall therapeutic efficacy. Therefore, the purpose of this review is to provide a comprehensive summary of the current methodologies for the preparation, isolation, and identification of BPs from traditional natural products in China and to classify the functions of discovered BPs. Insights from this review are expected to facilitate the development of targeted drugs and functional foods derived from traditional natural products in China in the future.

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“…The following structural parameters for amino acids were considered for calculating the descriptors of peptides: The substituent constants (қ), steric effects (ES,C) and molecular refractivity (MR) [22,23]. The values of these descriptors for twenty amino acids are listed in Table 2.…”

Section: Descriptorsmentioning

confidence: 99%

Determination and prediction of peptide mobilities by micellar electro-kinetic chromatography using adaptive neuro-fuzzy inference system as a feature selection method

sadi¹,

Khaledi

Jalali‐Heravi

2020

Anal. Method Environ. Chem. J.

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Mobility of 128 peptides composed of up to 14 amino acids is determined for sodium dodecyl sulfate (SDS) micellar systems using micellar electrokinetic chromatography (MEKC). The mobilities of these peptides are predicted using back propagation of error artificial neural networks (BP-ANNs). Adaptive neuro-fuzzy inference system (ANFIS) which can deal with linear and nonlinear phenomena is used to select the inputs of BP-ANN. A 3:4:1 BP-ANN model with four variables of Kappa substituent constant, Kappa(H), number of peptide bonds, (ln(N), molar refractivity of C-terminal, MRC, and steric effects at N-terminal, ES,N, which incorporate substituent, steric and molar refractivity effects as its inputs was developed. Comparison of Multiple Linear Regression (MLR) and ANN results shows the nonlinear characteristic of the phenomena. The nonlinear model was successful in predicting the mobilities of 120 peptides except for the ones (8 peptides) with negatively charged amino acids.

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LingZhi oligopeptides amino acid sequence analysis and anticancer potency evaluation

Abstract: The LingZhi (Ganoderma lucidum) has been used as a therapeutic agent for decades, but the antitumor potency of LingZhi oligopeptides (LZOs) was not well explored. In current study, ten novel LZOs were amino acid sequence identified and anticancer potency evaluated.

Cited by 4 publications

References 27 publications

UPLC-ESI-MS/MS-based widely targeted metabolomics reveals differences in metabolite composition among four Ganoderma species

UPLC-ESI-MS/MS-based widely targeted metabolomics reveals differences in metabolite composition among four Ganoderma species

The Research Progress of Bioactive Peptides Derived from Traditional Natural Products in China

Determination and prediction of peptide mobilities by micellar electro-kinetic chromatography using adaptive neuro-fuzzy inference system as a feature selection method

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