Linezolid resistance in clinical isolates of Staphylococcus epidermidis from German hospitals and characterization of two cfr-carrying plasmids

Abstract

To date, linezolid-resistant S. epidermidis have rarely been isolated from human clinical sources in Germany. Here, we describe the emergence and outbreaks of these strains. We detected previously described and novel point mutations in the 23S ribosomal genes. The cfr gene was only present in six isolates. However, this is the first known description of cfr incorporation into conjugative vectors; under selective pressure, these vectors could give reasonable cause for concern.

Dissemination ofStaphylococcus epidermidisST22 With Stable, High-Level Resistance to Linezolid and Tedizolid in the Greek-Turkish Region (2008–2016)

“…1 The LRSE-ST22 isolates have been associated with infection and colonization cases in Spain, France, Germany, and, particularly, Greece, and most of these LRSE-ST22 arise after treatment with linezolid. [1][2][3][4][5][6][7] In this study, we aimed to characterize the first methicillin-and linezolid-resistant S. epidermidis from a patient without previous linezolid exposure in Turkey and to assess its genetic similarity to the close geographical S. epidermidis from Greece.…”

Mechanisms of Horizontal Gene Transfer

“…As pSCFS3 plasmids were considered as non-conjugative vectors [94], this indirect demonstration of plasmid transmission questioned the nature of the HGT involved in the spread of these plasmids. In addition, an unexpected result was obtained analyzing the sequence of two cfr plasmids obtained in staphylococcal clinical isolates from German hospitals [108]. In this study, 6 cfr-positive strains were identiied among 36 linezolid-resistant S. epidermidis isolated between January 2012 and April 2013.…”

“…The presence of pSCFS7-like plasmids in clinical isolates from Germany may suggest the potential spread of these plasmids among European hospitals. Nevertheless, although pSCFS6 harbored the pGO1 tra and nes conjugative machinery, none of these genes were located in the pSCFS7 sequence, suggesting potential mobilization events of cfr environment onto diferent plasmids backbones [108].…”

Urinary tract infection due to beta-lactams-resistant Staphylococcus warneri: A case report

“…Sensitivity to quinolones, sulfamethoxazole/trimethoprim, nitrofurantoin and aminoglycosides vary among studies [8,9]. However, a recent paper from Germany has reported increased incidence of linezolid-resistance S. epidermidis isolates from hospitalized patient [10] …”