Linear SRY Transcript in Equine Testis

Hasegawa, Telhisa; Ishida, Michiyo; Harigaya, Toshio; Sato, Fumio; Ishida, Nobushige; Mukoyama, Harutaka

doi:10.1292/jvms.61.97

Cited by 18 publications

(17 citation statements)

References 17 publications

(21 reference statements)

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“…PCR for Y chromosome sequence was done using horse Y chromosome primers as previously described [Abe et al, 1999;Hasegawa et al, 1999;Bugno et al, 2003;Iannuzzi et al, 2004;Raudsepp et al, 2004].…”

Section: Sry Pcr Amplificationmentioning

confidence: 99%

Equine Disorders of Sexual Development in 17 Mares Including XX, SRY-Negative, XY, SRY-Negative and XY, SRY-Positive Genotypes

Villagómez

Lear

Chenier³

et al. 2010

Sex Dev

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We described the clinical, cytogenetic and molecular findings of 17 clinical equine cases presented for abnormal sexual development and infertility. Six horses with an enlarged clitoris had an XX, SRY-negative genotype, which displayed male-like behavior (adult individuals). Bilateral ovotestes were noted in 2 of those cases, while another case showed increased levels of circulating testosterone. Six horses with a female phenotype, including normal external genitalia, had an XY, SRY-negative genotype. These individuals had small gonads and an underdeveloped internal reproductive tract. Four horses with normal appearing external genitalia had an XY, SRY-positive genotype, 3 of them had hypoplastic testes and male-like behavior. In addition, one young filly with enlarged clitoris and hypoplastic testes had the same genotype but did not show male-like behavior due to her age. Three of these horses were related with 2 being siblings. These findings demonstrate the diversity of disorders of sexual development seen in the horse. Furthermore, they emphasize the need for further research to identify genes involved in abnormal sex determination and differentiation in the horse.

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Section: Sry Pcr Amplificationmentioning

confidence: 99%

Equine Disorders of Sexual Development in 17 Mares Including XX, SRY-Negative, XY, SRY-Negative and XY, SRY-Positive Genotypes

Villagómez

Lear

Chenier³

et al. 2010

Sex Dev

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“…Early molecular methods, such as Southern blots with human SRY probes, identified the first reported SRY -negative XY mare [Pailhoux et al, 1995]. Later, the equine SRY transcript sequence was reported by Hasegawa et al [1999] and used in designing the first PCR primers to report a SRY -negative XY mare [Mäkinen et al, 1999]. More recently, equine bacterial artificial chromosome probes and chromosome paints have been used for fluorescent in situ hybridization studies to determine the presence or absence of the X and Y chromosomes or SRY and other sex-linked genes [Breen et al, 1997;Bugno et al, 2009;Raudsepp et al, 2010;Villagomez et al, 2011].…”

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confidence: 99%

Disorders of Sexual Development in the Domestic Horse, Equus caballus

Lear

McGee²

2011

Sex Dev

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Abnormalities of sexual development causing infertility in horses have been investigated since the early 1970's. Conventional cytogenetic analysis by karyotyping has been the primary tool used to investigate these horses. Abnormalities have a broad range, from a phenotypically normal mare with gonadal dysgenesis to a horse with ambiguous external genitalia and internal male and female organs. Cytogenetic analysis can determine genetic sex but cannot identify mutations or deletions of genes involved in the sex determination pathway. Molecular technologies have been developed to confirm cytogenetic results and to aid in identifying the genetic causes of abnormal sex determination in horses. In this paper, we review the historical development of methods used to understand abnormal sexual development in the horse as well as summarize cases reported over the last 40-50 years.

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“…Thus far, only three genes (SRY, ZFY, and STS) are physically assigned to ECAY by fluorescence in situ hybridization (FISH) (6) and analysis of somatic hybrid cell panel (5). Except for identification of SRY as the sex determination factor (4,22), practically no information is available about the association of Y chromosome sequences with stallion infertility. This study reports the generation of a detailed physical map of the ECAY obtained by using a range of mapping approaches, including RH analysis; metaphase-, interphase-, and DNA fiber-FISH; chromosome walking; bacterial artificial chromosome (BAC) contig generation; and Abbreviations: RH, radiation hybrid; ECAY, Equus caballus Y chromosome; FISH, fluorescence in situ hybridization; BAC, bacterial artificial chromosome; Mb, megabases; PAR, pseudoautosomal region; STS, sequence-tagged site.…”

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confidence: 99%