Linear dose response of acrocentric chromosome associations to gamma irradiation in human lymphocytes

Samarth, Ravindra M.; Gandhi, Puneet; Chaudhury, N. K.

doi:10.1007/s00066-022-01978-3

Cited by 5 publications

(4 citation statements)

References 40 publications

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“…A dose-dependent increase in the number of cells exhibiting more than one DC formations was observed, with the notable nding that no instances of cells with two or more DC formations were observed up to 0.5 Gy, consistent with manual scoring observations. Statistical analysis con rmed a Poisson distribution for DC occurrences across all doses, consistent with earlier reports [10,11,23,33,34]. The dispersion index (u) and relative variance (σ²/Y) were calculated for each dose points, following the guidelines provided by the IAEA (Table 2) [24].…”

Section: Establishment Of Dose Response Curve For 60 Co-γ-ray Induced...supporting

confidence: 65%

“…The establishment of a precise calibration curve is crucial for accurate dose estimation [9]. This involves constructing a prede ned dose-response curve, where the occurrence of chromosomal aberrations correlates with radiation dose, typically described by linear-quadratic equations for low Linear Energy Transfer (LET) radiation and linear equations for high LET radiation [10]. It is imperative that the standard used for calibration matches or closely resembles the radiation quality of the speci c incident.…”

Section: Introductionmentioning

confidence: 99%

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Lab to Emergency: Establishment and validation of automated method for rapid biodosimetry

Vijayalakshmi,

Chaurasia,

Nair

et al. 2024

Preprint

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In inadvertent radiation exposure scenarios, accurate measurement of absorbed dose by biodosimetric techniques is crucial, especially when physical dosimetry is unavailable or in dispute. Chromosomal aberrations like dicentric (DC) and micronuclei (MN) are widely used for biodosimetry, with the former being the gold standard for individual dose assessment. However, both methods are labour-intensive requiring skilled analysis. This study aims to assess and compare the frequency and accuracy of DC and MN scoring through manual and automated methods. It investigates the establishment and validation of dose-response curves, in the dose range of 0-5 Gy, for 60Co-γ-ray-induced DC and MN using peripheral blood samples from three human volunteers. Statistical analysis confirmed dose-dependent increases in aberration frequencies, with Poisson distribution validation for all dose points. The manual and automated scoring methods yielded notable differences in linear and quadratic coefficients. Correlation analyses demonstrated substantial agreement between manual and automated scoring methods, particularly for MN (R2= 0.98). Towards validation, 16 dose-blinded samples were analysed for both endpoints using manual and automated scoring methods. Results yielded a close match between estimated and delivered doses, particularly evident with automated scoring (of both DC and MN) displaying superior accuracy for most of the doses. Notably, the variation was minimal, with -1.96% for DC and 2.85% for MN at 0.5 and 4 Gy, respectively. Automated scoring was approximately 15-20 times faster than manual scoring, suggestive of a faster practical option, especially in emergency scenarios such as criticality accidents and radiological emergencies, thus facilitating speedy triage and medical decisions.

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Section: Establishment Of Dose Response Curve For 60 Co-γ-ray Induced...supporting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

Lab to Emergency: Establishment and validation of automated method for rapid biodosimetry

Vijayalakshmi,

Chaurasia,

Nair

et al. 2024

Preprint

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“…Dose response relationship at acute dose radiation exposures above 100 mGy mostly remains linear in human lymphocytes using chromosomal aberrations, micronuclei and γH2AX foci analysis [ 35 – 38 ]. However, the shape of the dose response curve at chronic low dose exposures below 100 mGy is found to be non-linear in human lymphocytes using various DNA damage endpoints [ 29 , 43 – 45 ].…”

Section: Discussionmentioning

confidence: 99%

“…Extrapolation of risk from at high doses to low doses assumes that stochastic health effects and DNA damage at high and low doses are similar and the dose response is linear. It has been found that the induction of DNA DSBs may not always show a linear dose response due to IR exposure [ 35 – 38 ]. Although at high doses of IR exposure biological end points show linear dose response, but at low doses, it is not always linear.…”

Section: Introductionmentioning

confidence: 99%

Non-linear dose response of DNA double strand breaks in response to chronic low dose radiation in individuals from high level natural radiation areas of Kerala coast

et al. 2023

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Background The human population living in high level natural radiation areas (HLNRAs) of Kerala coast provide unique opportunities to study the biological effects of low dose and low dose rate ionizing radiation below 100 mGy. The level of radiation in this area varies from < 1.0 to 45 mGy/year. The areas with ≤ 1.50 mGy/year are considered as normal level natural radiation areas (NLNRA) and > 1.50 mGy/year, as high level natural radiation areas (HLNRA). The present study evaluated dose response relationship between DNA double strand breaks (DSBs) and background radiation dose in individuals residing in Kerala coast. Venous blood samples were collected from 200 individuals belonging to NLNRA (n = 50) and four dose groups of HLNRA; 1.51-5.0 mGy/year (n = 50), 5.01-10.0 mGy/year (n = 30), 10.01-15.0 mGy/year (n = 33), > 15.0 mGy/year (n = 37) with written informed consent. The mean dose of NLNRA and four HLNRA dose groups studied are 1.21 ± 0.21 (range: 0.57–1.49), 3.02 ± 0.95 (range: 1.57–4.93), 7.43 ± 1.48 (range: 5.01–9.75), 12.22 ± 1.47 (range: 10.21–14.99), 21.64 ± 6.28 (range: 15.26–39.88) mGy/year, respectively. DNA DSBs were quantified using γH2AX as a marker, where foci were counted per cell using fluorescence microscopy. Results Our results revealed that the frequency of γH2AX foci per cell was 0.090 ± 0.051 and 0.096 ± 0.051, respectively in NLNRA and HLNRA individuals, which were not significantly different (t198 = 0.33; P = 0.739). The frequency of γH2AX foci was observed to be 0.090 ± 0.051, 0.096 ± 0.051, 0.076 ± 0.036, 0.087 ± 0.042, 0.108 ± 0.046 per cell, respectively in different dose groups of ≤ 1.50, 1.51-5.0, 5.01-10.0, 10.01-15.0, > 15.0mGy/year (ANOVA, F4,195 = 2.18, P = 0.072) and suggested non-linearity in dose response. The frequency of γH2AX foci was observed to be 0.098 ± 0.042, 0.078 ± 0.037, 0.084 ± 0.042, 0.099 ± 0.058, 0.097 ± 0.06 and 0.114 ± 0.033 per cell in the age groups of ≤ 29, 30–34, 35–39, 40–44, 45–49 and ≥ 50 years, respectively (ANOVA, F5,194 = 2.17, P = 0.059), which suggested marginal influence of age on the baseline of DSBs. Personal habits such as smoking (No v/s Yes: 0.092 ± 0.047 v/s 0.093 ± 0.048, t198 = 0.13; P = 0.895) and drinking alcohol (No v/s Yes: 0.096 ± 0.052 v/s 0.091 ± 0.045, t198 = 0.62; P = 0.538) did not show any influence on DSBs in the population. Conclusion The present study did not show any increase in DSBs in different dose groups of HLNRA compared to NLNRA, however, it suggested a non-linear dose response between DNA DSBs and chronic low dose radiation.

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Co-occurrence of acrocentric chromosome associations with dicentric chromosomes in irradiated human lymphocytes

2023

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Linear dose response of acrocentric chromosome associations to gamma irradiation in human lymphocytes

Cited by 5 publications

References 40 publications

Lab to Emergency: Establishment and validation of automated method for rapid biodosimetry

Lab to Emergency: Establishment and validation of automated method for rapid biodosimetry

Non-linear dose response of DNA double strand breaks in response to chronic low dose radiation in individuals from high level natural radiation areas of Kerala coast

Co-occurrence of acrocentric chromosome associations with dicentric chromosomes in irradiated human lymphocytes

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