Let Mm,n be the space of m × n real or complex rectangular matrices. Two matrices A, B ∈ Mm,n are disjoint if A * B = 0n and AB * = 0m. In this paper, a characterization is given for linear maps Φ : Mm,n → Mr,s sending disjoint matrix pairs to disjoint matrix pairs, i.e., A, B ∈ Mm,n are disjoint ensures that Φ(A), Φ(B) ∈ Mr,s are disjoint. More precisely, it is shown that Φ preserves disjointness if and only if Φ is of the form